Abstract: The disclosure relates to the system comprising a combination of a primary bioreactor with a secondary bioreactor to convert a gaseous stream comprising CO and optionally H2, or CO2 and H2 to at least one target product. Bioreactors may be any combination of aerobic and anaerobic, batch and continuous. The bioreactors comprise fermenting microorganisms that may typically be bacterial or fungal.

Abstract: The disclosure relates to the combination of a primary fermentation that converts gas to acetate with a secondary fermentation that converts acetate to a target product. Preferably, the gas contains carbon dioxide, such that the disclosure enables the fixation of carbon dioxide into useful products. The fermentations may be any combination of aerobic and anaerobic, batch and continuous. The fermenting microorganisms may typically be bacterial or fungal.

Abstract: The disclosure relates to the combination of a primary fermentation that converts gas to acetate with a secondary fermentation that converts acetate to a target product. Preferably, the gas contains carbon dioxide, such that the disclosure enables the fixation of carbon dioxide into useful products. The fermentations may be any combination of aerobic and anaerobic, batch and continuous. The fermenting microorganisms may typically be bacterial or fungal.

Abstract: The invention provides a recombinant, carboxydotrophic Clostridium bacterium that expresses one or more of pyruvate:ferredoxin oxidoreductase (EC 1.2.7.1), acetolactate synthase (EC 2.2.1.6), and acetolactate decarboxylase (EC 4.1.1.5). The invention further provides a method of producing a fermentation product by fermenting the recombinant bacterium in the presence of a gaseous substrate comprising CO to produce one or more of ethanol, butanol, isopropanol, isobutanol, higher alcohols, butanediol, 2,3-butanediol, succinate, isoprenoids, fatty acids, biopolymers, and mixtures thereof.

Abstract: The invention provides genetically engineered microorganisms and methods for producing chorismate-derived products, such as para-hydroxybenzoic acid, salicylate, 2-aminobenzoate, 2,3-dihydroxybenzoate, and 4-hydroxycyclohexane carboxylic acid. Typically, the microorganism comprises at least one of (a) an exogenous chorismate pyruvate lyase, (b) an exogenous isochorismate synthase, (c) an exogenous isochorismate pyruvate lyase, and (d) a prephenate synthase comprising a disruptive mutation.

Abstract: Multi-stage, biological processes and systems for converting a C1 carbon source to desired end products are described. The processes comprise dividing a gaseous C1-containing substrate, in parallel, among multiple bioreactor stages. Liquid products are successively fed, in series, from a first bioreactor stage to downstream bioreactor stages. Operation can be simplified by avoiding the requirement for microorganism separation and recycle at each stage. In addition, overall vapor-liquid mass transfer for the combined stages is very favorable, leading to high end product productivity with comparably low byproduct metabolite productivity.

Abstract: A method is provided for controlling a metabolic profile of an anaerobic microbial fermentation culture. In particular, a metabolic profile of a fermentation process is controlled by controlling the amount of dissolved CO2 provided to a culture. Further provided is a method of producing one or more products by microbial fermentation of a gaseous substrate through feeding tail gas CO2 from a reactor to a second reactor, or by recycling tail gas CO2 to the same reactor.

Abstract: The invention provides genetically engineered microorganisms and methods for producing chorismate-derived products, such as para-hydroxybenzoic acid, salicylate, 2-aminobenzoate, 2,3-dihydroxybenzoate, and 4-hydroxycyclohexane carboxylic acid. Typically, the microorganism comprises at least one of (a) an exogenous chorismate pyruvate lyase, (b) an exogenous isochorismate synthase, (c) an exogenous isochorismate pyruvate lyase, and (d) a prephenate synthase comprising a disruptive mutation.

Abstract: The invention provides a recombinant, carboxydotrophic Clostridium bacterium that expresses one or more of pyruvate:ferredoxin oxidoreductase (EC 1.2.7.1), acetolactate synthase (EC 2.2.1.6), and acetolactate decarboxylase (EC 4.1.1.5). The invention further provides a method of producing a fermentation product by fermenting the recombinant bacterium in the presence of a gaseous substrate comprising CO to produce one or more of ethanol, butanol, isopropanol, isobutanol, higher alcohols, butanediol, 2,3-butanediol, succinate, isoprenoids, fatty acids, biopolymers, and mixtures thereof.

Abstract: Multi-stage, biological processes and systems for converting a C1 carbon source to desired end products are described. The processes comprise dividing a gaseous C1-containing substrate, in parallel, among multiple bioreactor stages. Liquid products are successively fed, in series, from a first bioreactor stage to downstream bioreactor stages. Operation can be simplified by avoiding the requirement for microorganism separation and recycle at each stage. In addition, overall vapor-liquid mass transfer for the combined stages is very favorable, leading to high end product productivity with comparably low byproduct metabolite productivity.

Abstract: Methods and systems for the production of one or more lipid products from a gaseous substrate using a two stage fermentation process are provided. The method comprises providing a gaseous substrate comprising CO2 and H2 to a primary bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce an acid such as acetate. The acid, e.g. acetate from the primary bioreactor is then provided to a secondary bioreactor, where it is used as a substrate for fermentation to lipids by one or more yeasts.

Abstract: The invention relates to a process for optimizing the production of ethanol by microbial fermentation, particularly microbial fermentation of substrates comprising CO. The process involves monitoring the hydrogen production by the microorganism, determining an optimum hydrogen production and adjusting the substrate supply rate in response to a change in the hydrogen production thereby keeping the hydrogen production within a desired range.

Abstract: A method is provided for controlling a metabolic profile of an anaerobic microbial fermentation culture. In particular, a metabolic profile of a fermentation process is controlled by controlling the amount of dissolved CO2 provided to a culture. Further provided is a method of producing one or more products by microbial fermentation of a gaseous substrate through feeding tail gas CO2 from a reactor to a second reactor, or by recycling tail gas CO2 to the same reactor.

Abstract: The invention provides method for producing butanediol and specifically 2,3-butanediol by anaerobic fermentation of a gaseous substrate comprising CO by a culture of at least one micro-organism. In accordance with particular methods of the invention, 2,3-butanediol is produced by anaerobic fermentation of substrates including carbohydrates and carbon monoxide. The invention further provides for the up-regulation of a native 2,3-butanediol dehydrogenase gene in the at least one micro-organism. The 2,3-butanediol can be further converted to compounds such as butene(s), butadiene and methyl ethyl ketone. These compounds can be further converted to chemical products such as 2-butanol.

Abstract: The invention relates to a process for optimising the production of ethanol by microbial fermentation, particularly microbial fermentation of substrates comprising CO. The process involves monitoring the hydrogen production by the microorganism, determining an optimum hydrogen production and adjusting the substrate supply rate in response to a change in the hydrogen production thereby keeping the hydrogen production within a desired range.

Abstract: The invention relates to improvements in the production of alcohols by microbial fermentation, particularly to production of alcohols by microbial fermentation of a substrate comprising CO. It more particularly relates to the addition of an inorganic sulfur source to a fermentation system such that one or more micro-organisms convert a substrate comprising CO to alcohols. In a particular embodiment, a microbial culture is provided with sodium polysulfide, wherein a substrate comprising CO is converted to products including ethanol and 2,3-butanediol.

Abstract: Methods and systems for the production of one or more lipid products from a gaseous substrate using a two stage fermentation process are provided. The method comprises providing a gaseous substrate comprising CO2 and H2 to a primary bioreactor containing a culture or one or more microorganisms, and fermenting the substrate to produce an acid such as acetate. The acid, e.g. acetate from the primary bioreactor is then provided to a secondary bioreactor, where it is used as a substrate for fermentation to lipids by one or more yeasts.