Abstract: The present disclosure relates to a field of memory technical, and in particular to a magnetoresistive device, a method for changing a resistance state of the magnetoresistive device, and a synapse learning module. The magnetoresistive device includes a top electrode, a ferromagnetic reference layer, a tunneling layer, a ferromagnetic free layer, a spin-orbit coupling layer, and a bottom electrode that are arranged in sequence along a preset direction, where the spin-orbit coupling layer includes a first thickness region and a second thickness region distributed alternately, and a thickness of the first thickness region is different form a thickness of the second thickness region; and the ferromagnetic free layer includes a pinning region, and a position of the pinning region is in one-to-one correspondence with a position of the first thickness region.

Abstract: The present invention provides a pyruvate-responsive biosensor and a construction method and use thereof. In the present invention, pyruvate-responsive biosensors with different dynamic ranges are successfully constructed by optimizing the PdhR binding sequence inserted on the P43 promoter and optimizing the insertion site, wherein the minimum increase in dynamic range is by 0.6 time, and the maximum increase is by 30.7 times. The pyruvate-responsive biosensors are useful in the precise control of the expression of each gene in the cell. Since pyruvate is a key metabolite of central carbon in the cells, these biosensors are capable of dynamically regulating the expression level of intracellular genes according to changes in the content of pyruvate in the cells, thereby achieving the dynamic control of intracellular metabolic flux. The pyruvate biosensor obtained in the present invention has a good specificity, and a response range to pyruvate of 10-35 nmol/g DCW.

Abstract: A vacuum liquid-filling needle includes a needle shaft (2) and a pressure relief valve (3). The pressure relief valve is disposed within the needle shaft at a first end (21) thereof, and the needle shaft is able to connect at a second end (22) thereof to a pipe for supplying a solution to be filled. The pressure relief valve defines an internal cavity, in which a first slide valve and a second slide valve are disposed so as to be axially spaced apart from each other and both movable relative to the internal cavity. The valve body defines an inflow channel (33), an inflow port (34), an outflow port (35), an outflow channel (37) and exit apertures (38). Each of the inflow channel and the outflow channel is formed by a blind bore, and both the inflow channel and the outflow channel axially extend along the valve body. This vacuum liquid-filling needle can prevent undesired accidental efflux of the solution while providing depressurization and pressure stabilization effects during filling of the solution.

Abstract: Disclosed is a combustion system design method based on a target heat release rate, which belongs to the technical field of diesel engine combustion chamber design. The method includes: obtaining an ideal heat release rate based on Sabathe-Miller cycle; simulating the ideal heat release rate based on a double-Wiebe function and obtaining the target heat release rate; constructing a mapping relation among the heat release rate, piston geometric parameters and fuel injection parameters, which includes target start of combustion being an function of fuel injection timing and ignition delay, premixed combustion parameters being functions of throat radius, injection pressure and nozzle diameter, and diffusion combustion being a function of piston pit depth; solving target piston geometric parameters and target fuel injection parameters based on the mapping relation; and then designing a combustion system.

Abstract: A composting device includes a stirring device, a conveyor belt and a decomposing device. The stirring device, the conveyor belt and the decomposing device are connected successively. A composting method is further provided. An efficient device that can mix livestock and poultry manure, straws and additives fully and uniformly is researched and developed, a conventional method is broken, new innovation is achieved, and the efficiency is improved.

Abstract: An autoinducer-2 (AI-2) molecular response-based starting element and an Escherichia coli (E. coli) dynamic regulation system and method constructed thereby are provided. A cell density-dependent starting element PJ23119-LsrR-PlsrA based on an AI-2 molecular response is constructed. The element can be used to self-induce the expression of dCpf1, and crRNAs of different target genes are further assembled, such that the self-inducible element can be used for dCpf1-CRP to achieve the dynamic regulation of genes in a synthesis pathway. In the present disclosure, vectors pACYDuet-PJ23119-LsrR-PlsrA-dCpf1-CRP, pRSFDuet-GFP-mCherry, and pETDuet-crRNA can be constructed to simultaneously achieve the transcriptional activation and inhibition of different genes. The construction method of recombinant E. coli in the present disclosure is simple and has promising application prospects.

Abstract: A reconfigurable PUF device based on fully electric field-controlled domain wall motion includes a voltage control layer, upper electrodes, a lower electrode, antiferromagnetic pinning layers, and a magnetic tunnel junction (MTJ). The MTJ includes, from bottom to top, a ferromagnetic reference layer, a potential barrier tunneling layer and a ferromagnetic free layer. In the device, an energy potential well is formed in a middle portion of the ferromagnetic free layer by applying a voltage to the voltage control layer to control magnetic anisotropy, and a current is fed into either of the upper electrodes to drive generation of the magnetic domain walls and pin the magnetic domain walls to the potential well. After the voltage is removed, the potential well is lowered so that the magnetic domain walls are in a metastable state, thereby either a high resistance state or a low resistance state is randomly obtained.

Abstract: The present invention provides an acid-tolerant Saccharomyces cerevisiae strain and use thereof. By using exogenously added malic acid as a stress, an acid-tolerant mutant S. cerevisiae strain MTPfo-4 is obtained by directed evolution screening in the laboratory, which tolerates a minimum pH of 2.44. The mutant strain MTPfo-4, tolerant to multiple organic acids, has an increased tolerance to exogenous malic acid of up to 86.6 g/L. The mutant strain MTPfo-4 obtained is further identified. The mutant strain grows stably and well, and can tolerate a variety of organic acids (lactic acid, malic acid, succinic acid, fumaric acid, citric acid, gluconic acid, and tartaric acid). It also has a strong tolerance to inorganic acids (HCl and H3PO4). This is difficult to achieve in the existing research and reports of S. cerevisiae. The strain is intended to be used as an acid-tolerant chassis cell factory for producing various short-chain organic acids.

Abstract: The disclosure discloses Bacillus subtilis for producing N-acetylneuraminic acid and application thereof, and belongs to the field of genetic engineering. The disclosure optimizes the expression levels of key enzymes in N-acetylneuraminic acid synthesis pathways on genome through promoters of different strength, reduces the protein synthesis pressure caused by the expression of enzymes on cells, and further integrates the three N-acetylneuraminic acids in a same Bacillus subtilis engineering strain. Bacillus subtilis with improved N-acetylneuraminic acid production is obtained, and the production reaches 10.4 g/L at the shake flask level, laying a foundation for further improving the NeuAc production from Bacillus subtilis.

Abstract: Provided is a free piston generator based on a split thermodynamic cycle, which belongs to the technical field of power energy. The present disclosure solves the problem of low power generation efficiency of an existing free piston generator. The free piston generator includes a linear generator set and two internal combustion engine sets arranged at two ends of the linear generator set. Air is first subjected to first-stage compression by the low-pressure cylinder set in the internal combustion engine sets and is then subjected to second-stage compression in the high-pressure cylinders, so that the intake pressure of an internal combustion engine is effectively increased, which is favorable for increasing the average effective pressure in a work process, thereby improving the thermal efficiency and the power generation efficiency of the free piston generator.

Abstract: A magnetoresistive memory cell includes a first magnetic tunnel junction, a second magnetic tunnel junction and a metal layer. The first magnetic tunnel junction and the second magnetic tunnel junction each are disposed on the metal layer; the metal layer is configured to pass write current, a projection line of an easy axis of the first magnetic tunnel junction on a plane where the metal layer is located forms a first angle against a direction of the write current, and a projection line of an easy axis of the second magnetic tunnel junction on the plane where the metal layer is located forms a second angle against a direction opposite to the direction of the write current; the first angle and the second angle are all less than 90°; the first magnetic tunnel junction and the second magnetic tunnel junction are configured to pass read current.

Abstract: The disclosure discloses recombinant Bacillus subtilis for synthesizing guanosine diphosphate fucose and a construction method and application thereof. The recombinant Bacillus subtilis is obtained by intensively expressing guanylate kinase and nucleotide diphosphokinase genes and expressing exogenous fucokinase and phosphate guanylyltransferase genes in a genome of Bacillus subtilis 168. According to the disclosure, a bacterial strain for synthesizing the guanosine diphosphate fucose is obtained by reconstructing the Bacillus subtilis 168, with a volume of intracellular accumulation up to 196.15 g/L. According to the disclosure, by intensively expressing the guanylate kinase and nucleotide diphosphokinase genes, and enhancing the supply of intracellular GDP-L-fucose composition cofactors, the synthesis of the guanosine diphosphate fucose is promoted. The construction method for the recombinant Bacillus subtilis of the disclosure is simple and convenient to use, thus having good application prospects.

Abstract: The present invention provides a recombinant Corynebacterium glutamicum producing N-acetylglucosamine and use thereof. The recombinant Corynebacterium glutamicum is obtained by overexpressing, in Corynebacterium glutamicum, the transcription factor SugR derived therefrom. The recombinant Corynebacterium glutamicum of the present invention increases the production of acetylglucosamine to up to 26 g/L, and lays a foundation for further metabolic engineering of Corynebacterium glutamicum to produce glucosamine.

Abstract: The present disclosure provides an apparatus for testing spray and combustion performance of an internal-combustion engine based on a rapid compression-expansion machine, which belongs to the testing field of internal-combustion engines. It includes a driving mechanism, a transmission mechanism, an engine cylinder, an air intake system and an exhaust system. The engine cylinder includes a piston, a cylinder block, a dual-fuel cylinder head and a multi-injector assembly. The piston is disposed in the cylinder block. The driving mechanism is connected with the piston through the transmission mechanism and drives the piston to linearly move back and forth along a vertical direction of the cylinder block. The dual-fuel cylinder head is connected to an upper part of the cylinder block. The multi-fuel injection assembly includes a plurality of injectors. The plurality of injectors are of the same type and are all disposed on the dual-fuel cylinder head.

Abstract: The present disclosure provides a six-cylinder opposed free piston internal combustion engine generator. The generator comprises two free piston internal combustion engine sets, one opposed piston internal combustion engine set and two linear generator sets. Air entering cylinders is subjected to first-stage compression in low-pressure cylinder sets in the free piston internal combustion engine sets and the opposed piston internal combustion engine set and then subjected to second-stage compression in high-pressure cylinder sets, and a high pressure gas produced after the combustion is subjected to first-stage expansion in the high-pressure cylinder sets and then subjected to second-stage expansion in the low-pressure cylinder sets.

Abstract: Provided is a free piston generator based on a split thermodynamic cycle, which belongs to the technical field of power energy. The present disclosure solves the problem of low power generation efficiency of an existing free piston generator. The free piston generator includes a linear generator set and two internal combustion engine sets arranged at two ends of the linear generator set. Air is first subjected to first-stage compression by the low-pressure cylinder set in the internal combustion engine sets and is then subjected to second-stage compression in the high-pressure cylinders, so that the intake pressure of an internal combustion engine is effectively increased, which is favorable for increasing the average effective pressure in a work process, thereby improving the thermal efficiency and the power generation efficiency of the free piston generator.

Abstract: The present disclosure provides an apparatus for testing spray and combustion performance of an internal-combustion engine based on a rapid compression-expansion machine, which belongs to the testing field of internal-combustion engines. The present disclosure solves the problems that the existing test of the internal-combustion engine testing apparatus having a complex structure, is poor in adaptability, cannot be observed easily, and cannot meet the requirements of multi-fuel injection and multi-injection-pressure tests. It includes a driving mechanism, a transmission mechanism, an engine cylinder, an air intake system and an exhaust system. The engine cylinder includes a piston, a cylinder block, a dual-fuel cylinder head and a multi-injector assembly. The piston is disposed in the cylinder block. The driving mechanism is connected with the piston through the transmission mechanism and drives the piston to linearly move back and forth along a vertical direction of the cylinder block.

Abstract: The present disclosure provides a six-cylinder opposed free piston internal combustion engine generator. The generator comprises two free piston internal combustion engine sets, one opposed piston internal combustion engine set and two linear generator sets. Air entering cylinders is subjected to first-stage compression in low-pressure cylinder sets in the free piston internal combustion engine sets and the opposed piston internal combustion engine set and then subjected to second-stage compression in high-pressure cylinder sets, and a high pressure gas produced after the combustion is subjected to first-stage expansion in the high-pressure cylinder sets and then subjected to second-stage expansion in the low-pressure cylinder sets.

Abstract: The disclosure is an engine simulation test device capable of realizing an ultrahigh compression temperature and pressure, belonging to the field of a diesel engine high-temperature and high-pressure system, solving the problems that the existing engine simulation test cannot achieve ultra-high compression temperature and pressure, and the temperature and pressure are not adjustable. It includes a compressed air inlet mechanism, a nitrogen gas inlet mechanism, a pressure stabilizing mechanism, a cyclic heating mechanism, an air inlet mechanism and a fast compressor mechanism.

Abstract: A free piston generator based on a rigid synchronous transmission system is provided, which belongs to the technical field of power energy. The present disclosure solves the problems of low power generation efficiency and low stability of the existing free piston generator. The free piston generator based on the rigid synchronous transmission system includes a first linear generator set, a second linear generator set, a rigid synchronous transmission assembly, two high-pressure cylinders arranged at two ends of the first linear generator set, and two low-pressure cylinders arranged at two ends of the second linear generator set.