Patents by Inventor Louis Masi
Louis Masi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11857682Abstract: A method of making a modified hyaluronate composition including providing an aqueous solution of uncrosslinked hyaluronic acid or sodium hyaluronate in unbuffered 135 to 200 mM NaCl, crosslinking the uncrosslinked hyaluronic acid or sodium hyaluronate in the aqueous solution with a dialdehyde or disulfide crosslinking agent to provide crude modified hyaluronate having a soluble fraction and a crosslinked fraction with a 10% to 98% degree of crosslinking, centrifuging the crude modified hyaluronate and removing at least a portion of the soluble fraction, and optionally repeating the centrifuging and removing until the modified hyaluronate has less than 10% by weight of the soluble fraction based on the total weight of hyaluronate, with a soluble fraction polydispersity (Mw/Mn) of 1 to 1.6, an Rh of the crosslinked fraction of 150 nm to 2000 nm, and an Rz of the crosslinked fraction of 50 nm to 700 nm, wherein Rh>Rz.Type: GrantFiled: April 13, 2021Date of Patent: January 2, 2024Assignee: AMC Group LLCInventor: Louis Masi
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Publication number: 20210308055Abstract: A method of making a modified hyaluronate composition including providing an aqueous solution of uncrosslinked hyaluronic acid or sodium hyaluronate in unbuffered 135 to 200 mM NaCl, crosslinking the uncrosslinked hyaluronic acid or sodium hyaluronate in the aqueous solution with a dialdehyde or disulfide crosslinking agent to provide crude modified hyaluronate having a soluble fraction and a crosslinked fraction with a 10% to 98% degree of crosslinking, centrifuging the crude modified hyaluronate and removing at least a portion of the soluble fraction, and optionally repeating the centrifuging and removing until the modified hyaluronate has less than 10% by weight of the soluble fraction based on the total weight of hyaluronate, with a soluble fraction polydispersity (Mw/Mn) of 1 to 1.6, an Rh of the crosslinked fraction of 150 nm to 2000 nm, and an Rz of the crosslinked fraction of 50 nm to 700 nm, wherein Rh>Rz.Type: ApplicationFiled: April 13, 2021Publication date: October 7, 2021Inventor: Louis Masi
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Patent number: 11058640Abstract: A method of making a modified hyaluronate composition including providing an aqueous solution of uncrosslinked hyaluronic acid or sodium hyaluronate in unbuffered 135 to 200 mM NaCl, crosslinking the uncrosslinked hyaluronic acid or sodium hyaluronate in the aqueous solution with a dialdehyde or disulfide crosslinking agent to provide crude modified hyaluronate having a soluble fraction and a crosslinked fraction with a 10% to 98% degree of crosslinking, centrifuging the crude modified hyaluronate and removing at least a portion of the soluble fraction, and optionally repeating the centrifuging and removing until the modified hyaluronate has less than 10% by weight of the soluble fraction based on the total weight of hyaluronate, with a soluble fraction polydispersity (Mw/Mn) of 1 to 1.6, an Rh of the crosslinked fraction of 150 nm to 2000 nm, and an Rz of the crosslinked fraction of 50 nm to 700 nm, wherein Rh>Rz.Type: GrantFiled: April 7, 2020Date of Patent: July 13, 2021Assignee: AMC GROUP, LLCInventor: Louis Masi
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Patent number: 10947493Abstract: Methods are disclosed for separating beads and cells from a host fluid. The method includes flowing a mixture containing the host fluid, the beads, and the cells through an acoustophoretic device having an ultrasonic transducer including a piezoelectric material driven by a drive signal to create a multi-dimensional acoustic standing wave. A drive signal is sent to drive the at least one ultrasonic transducer to create the multi-dimensional acoustic standing wave. A recirculating fluid stream having a tangential flow path is located substantially tangential to the standing wave and separated therefrom by an interface region. A portion of the cells pass through the standing wave, and the beads are held back from the standing wave in the recirculating fluid stream at the interface region. Also disclosed is an acoustophoretic device having a coolant inlet adapted to permit the ingress of a cooling fluid into the device for cooling the transducer.Type: GrantFiled: August 29, 2017Date of Patent: March 16, 2021Assignee: FloDesign Sonics, Inc.Inventors: Bart Lipkens, Rudolf Gilmanshin, Louis Masi, Benjamin Ross-Johnsrud, Erik Miller, Walter M. Presz, Jr., Thomas J. Kennedy, III
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Publication number: 20200399583Abstract: Methods are disclosed for separating beads and cells from a host fluid. The method includes flowing a mixture containing the host fluid, the beads, and the cells through an acoustophoretic device having an ultrasonic transducer including a piezoelectric material driven by a drive signal to create a multi-dimensional acoustic standing wave. A drive signal is sent to drive the at least one ultrasonic transducer to create the multi-dimensional acoustic standing wave. A recirculating fluid stream having a tangential flow path is located substantially tangential to the standing wave and separated therefrom by an interface region. A portion of the cells pass through the standing wave, and the beads are held back from the standing wave in the recirculating fluid stream at the interface region. Also disclosed is an acoustophoretic device having a coolant inlet adapted to permit the ingress of a cooling fluid into the device for cooling the transducer.Type: ApplicationFiled: May 28, 2020Publication date: December 24, 2020Inventors: Bart Lipkens, Rudolf Gilmanshin, Louis Masi, Benjamin Ross-Johnsrud, Erik Miller, Walter M. Presz, JR., Thomas J. Kennedy, III
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Patent number: 10662404Abstract: A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.Type: GrantFiled: March 31, 2017Date of Patent: May 26, 2020Assignee: FloDesign Sonics, Inc.Inventors: Bart Lipkens, Louis Masi, Stanley Kowalski, III, Walter M. Presz, Jr., Jason Dionne, Brian Dutra, Ari Mercado, Thomas J. Kennedy, III, Arthur Martin
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Patent number: 10640760Abstract: Multi-stage acoustophoretic devices for continuously separating a second fluid or a particulate from a host fluid are disclosed. Methods of operating the multi-stage acoustophoretic devices are also disclosed. The systems may include multiple acoustophoretic devices fluidly connected to one another in series, each acoustophoretic device comprising a flow chamber, an ultrasonic transducer capable of creating a multi-dimensional acoustic standing wave, and a reflector. The systems can further include pumps and flowmeters.Type: GrantFiled: May 3, 2017Date of Patent: May 5, 2020Assignee: FloDesign Sonics, Inc.Inventors: Bart Lipkens, Walter M. Presz, Jr., Brian Dutra, Rudolf Gilmanshin, Jason Dionne, Chris Leidel, Louis Masi, Goutam Ghoshal, Kedar Chitale
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Patent number: 10350514Abstract: An acoustic standing wave is utilized to separate components from a multi-component fluid, such as oil from an oil-water mixture, in a fluid flow scheme with an acoustophoresis device. For example, the flow scheme and device allows for trapping of the oil as the oil coalesces, agglomerates, and becomes more buoyant than the water.Type: GrantFiled: June 13, 2017Date of Patent: July 16, 2019Assignee: FloDesign Sonics, Inc.Inventors: Bart Lipkens, Jason Dionne, Ari Mercado, Brian Dutra, Walter M. Presz, Jr., Thomas J. Kennedy, III, Louis Masi
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Publication number: 20180066224Abstract: Methods are disclosed for separating beads and cells from a host fluid. The method includes flowing a mixture containing the host fluid, the beads, and the cells through an acoustophoretic device having an ultrasonic transducer including a piezoelectric material driven by a drive signal to create a multi-dimensional acoustic standing wave. A drive signal is sent to drive the at least one ultrasonic transducer to create the multi-dimensional acoustic standing wave. A recirculating fluid stream having a tangential flow path is located substantially tangential to the standing wave and separated therefrom by an interface region. A portion of the cells pass through the standing wave, and the beads are held back from the standing wave in the recirculating fluid stream at the interface region. Also disclosed is an acoustophoretic device having a coolant inlet adapted to permit the ingress of a cooling fluid into the device for cooling the transducer.Type: ApplicationFiled: August 29, 2017Publication date: March 8, 2018Inventors: Bart Lipkens, Rudolf Gilmanshin, Louis Masi, Benjamin Ross-Johnsrud, Erik Miller, Walter M. Presz, JR., Thomas J. Kennedy, III
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Publication number: 20170321208Abstract: Multi-stage acoustophoretic devices for continuously separating a second fluid or a particulate from a host fluid are disclosed. Methods of operating the multi-stage acoustophoretic devices are also disclosed. The systems may include multiple acoustophoretic devices fluidly connected to one another in series, each acoustophoretic device comprising a flow chamber, an ultrasonic transducer capable of creating a multi-dimensional acoustic standing wave, and a reflector. The systems can further include pumps and flowmeters.Type: ApplicationFiled: May 3, 2017Publication date: November 9, 2017Inventors: Bart Lipkens, Walter M. Presz, JR., Brian Dutra, Rudolf Gilmanshin, Jason Dionne, Chris Leidel, Louis Masi, Goutam Ghoshal
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Patent number: 9789222Abstract: Described herein are injectable alloplastic implant compositions that are particularly useful for soft tissue defect augmentation. The compositions include microparticles, such as polymethylmethacrylate particles, and collagen as a suspending agent, wherein the collagen contains a reduced amount of low molecular weight gelatine compared to high molecular weight collagen. By controlling the molecular weight of the collagen in the compositions, the injectability, stability, and antigenicity of the alloplastic implant compositions can be improved.Type: GrantFiled: April 27, 2016Date of Patent: October 17, 2017Assignee: SUNEVA MEDICAL, INC.Inventor: Louis Masi
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Patent number: 9783775Abstract: A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.Type: GrantFiled: February 14, 2017Date of Patent: October 10, 2017Assignee: FloDesign Sonics, Inc.Inventors: Bart Lipkens, Louis Masi, Stanley Kowalski, III, Walter M. Presz, Jr., Jason Dionne, Brian Dutra, Ari Mercado, Thomas J. Kennedy, III, Arthur Martin
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Publication number: 20170282097Abstract: An acoustic standing wave is utilized to separate components from a multi-component fluid, such as oil from an oil-water mixture, in a fluid flow scheme with an acoustophoresis device. For example, the flow scheme and device allows for trapping of the oil as the oil coalesces, agglomerates, and becomes more buoyant than the water.Type: ApplicationFiled: June 13, 2017Publication date: October 5, 2017Inventors: Bart Lipkens, Jason Dionne, Ari Mercado, Brian Dutra, Walter M. Presz, JR., Thomas J. Kennedy, III, Louis Masi
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Patent number: 9752114Abstract: A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.Type: GrantFiled: March 8, 2017Date of Patent: September 5, 2017Assignee: FloDesign Sonics, IncInventors: Bart Lipkens, Louis Masi, Stanley Kowalski, III, Walter M. Presz, Jr., Jason Dionne, Brian Dutra, Ari Mercado, Thomas J. Kennedy, III, Arthur Martin, John Rozembersky
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Patent number: 9745548Abstract: Methods are disclosed for separating beads and cells from a host fluid. The method includes flowing a mixture containing the host fluid, the beads, and the cells through an acoustophoretic device having an ultrasonic transducer including a piezoelectric material driven by a drive signal to create a multi-dimensional acoustic standing wave. A drive signal is sent to drive the at least one ultrasonic transducer to create the multi-dimensional acoustic standing wave. A recirculating fluid stream having a tangential flow path is located substantially tangential to the standing wave and separated therefrom by an interface region. A portion of the cells pass through the standing wave, and the beads are held back from the standing wave in the recirculating fluid stream at the interface region. Also disclosed is an acoustophoretic device having a coolant inlet adapted to permit the ingress of a cooling fluid into the device for cooling the transducer.Type: GrantFiled: January 30, 2017Date of Patent: August 29, 2017Assignee: FloDesign Sonics, Inc.Inventors: Bart Lipkens, Rudolf Gilmanshin, Louis Masi, Benjamin Ross-Johnsrud, Erik Miller, Walter M. Presz, Jr., Thomas J. Kennedy, III
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Patent number: 9738867Abstract: A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.Type: GrantFiled: February 14, 2017Date of Patent: August 22, 2017Assignee: FloDesign Sonics, Inc.Inventors: Bart Lipkens, Louis Masi, Stanley Kowalski, III, Walter M. Presz, Jr., Jason Dionne, Brian Dutra, Ari Mercado, Thomas J. Kennedy, III, Arthur Martin
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Publication number: 20170204360Abstract: A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.Type: ApplicationFiled: March 31, 2017Publication date: July 20, 2017Inventors: Bart Lipkens, Louis Masi, Stanley Kowalski, III, Walter M. Presz, JR., Jason Dionne, Brian Dutra, Ari Mercado, Thomas J. Kennedy, III, Arthur Martin
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Publication number: 20170191022Abstract: A perfusion bioreactor includes at least one ultrasonic transducer that can acoustically generate a multi-dimensional standing wave. The standing wave can be used to retain cells in the bioreactor, and can also be utilized to dewater or further harvest product from the waste materials produced in a bioreactor.Type: ApplicationFiled: March 22, 2017Publication date: July 6, 2017Inventors: Bart Lipkens, Louis Masi, Stanley Kowalski, III, Walter M. Presz, JR., Jason Dionne, Brian Dutra, Ari Mercado, Thomas J. Kennedy, III, Arthur Martin, John Rozembersky
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Patent number: 9688958Abstract: A series of multi-dimensional acoustic standing waves is set up inside a growth volume of a bioreactor. The acoustic standing waves are used to hold a cell culture in place as a nutrient fluid stream flows through the cell culture. The nutrient fluid stream dislodges some cells from the cell culture, which can then be recovered for cell therapy applications. The cell culture continues to expand and reproduce, permitting continuous recovery of cells from the bioreactor.Type: GrantFiled: August 23, 2016Date of Patent: June 27, 2017Assignee: FLODESIGN SONICS, INC.Inventors: Thomas J. Kennedy, III, Bart Lipkens, Louis Masi, Stanley Kowalski, Chris Leidel
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Publication number: 20170173498Abstract: An acoustic standing wave is utilized to separate components from a multi-component fluid, such as oil from an oil-water mixture, in a fluid flow scheme with an acoustophoresis device. For example, the flow scheme and device allows for trapping of the oil as the oil coalesces, agglomerates, and becomes more buoyant than the water.Type: ApplicationFiled: January 19, 2016Publication date: June 22, 2017Inventors: Bart Lipkens, Jason Dionne, Ari Mercado, Brian Dutra, Walter M. Presz, JR., Thomas J. Kennedy, III, Louis Masi