Abstract: The present invention relates to an ultrasensitive assay platform for the detection of nucleic acids such as microRNAs (miRNAs), which are important biomarker for diseases including cancer. The platform allows high throughput detection of multiple nucleic acid sequences miRNAs on the single-molecule level using fluorescence labeling, molecular barcoding, and flow based detection and multiparametric data analysis.

Abstract: The present invention relates to an ultrasensitive assay platform for the detection of nucleic acids such as microRNAs (miRNAs), which are important biomarker for diseases including cancer. The platform allows high throughput detection of multiple nucleic acid sequences miRNAs on the single-molecule level using fluorescence labeling, molecular barcoding, and flow based detection and multiparametric data analysis.

Abstract: A method and system is provided which uses cleavable linkers to detect an analyte in an immunoassay. The use of linkers in ELISA and similar immunoassay protocols allows for a reduction in wash steps, incubation time, and potential for user error. The linkers create an environment that allows for intramolecular binding kinetics for quickly binding an analyte to two antibodies. The system works with ELISA and other similar protocols, and one embodiment of the invention does not require the binding of antibodies to a solid support. The disclosure also provides a method of making the system of cleavable linkers for use in a variety of immunoassays.