Abstract: The invention relates to a fungus or a mushroom and to a method of producing it wherein the fungus/mushroom has an increased expression level of a polypeptide and/or has a decreased expression level of a polypeptide, wherein the polypeptide comprises an amino acid sequence that has at least 40% amino acid identity or similarity with a sequence selected from SEQ ID NO:1-200 and/or that has at least 50% amino acid identity or similarity with a sequence selected from SEQ ID NO:201-208.

Abstract: The invention relates to the field of genetic engineering, more specifically to the field of production of glycoproteins, more specifically to the engineering of the glycosylation pattern of glycoproteins. Provided is a method for producing a glycoprotein in a recombinant host cell, comprising allowing expression of said glycoprotein in a basidiomycete host provided with N-acetylglucosamine: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase (GnT-I; EC 2.4.1.101) activity. Also provided is a basidiomycente host cell expressing GNT-I, preferably human GnT-I and use thereof for the manufacture of (mammalian) proteins carrying complex N-glycans, such as (monoclonal) antibodies.