Abstract: This invention relates to an anti-freeze separator assembly connected to a piece of equipment in outdoor freezing conditions in order to take a fluid, such as water, and treat same for subsequent disposal without freezing. The anti-freeze separator assembly includes 1) a main container housing; 2) a material retainer member mounted within the main container housing to receive and hold a treatment chemical therein; 3) an enclosure cap assembly releasably mounted on the main container housing; 4) a main fluid inlet assembly connected to the main container housing to transfer a fluid to be treated therein; and 5) a main discharge assembly connected to the main container housing to transfer a treat fluid therefrom. The main discharge assembly includes a discharge pipe member connected to a transfer pipe assembly with a diaphragm valve assembly therebetween.