Patents by Inventor Lyle J. Arnold, Jr.
Lyle J. Arnold, Jr. has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11859238Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.Type: GrantFiled: January 7, 2019Date of Patent: January 2, 2024Assignee: GEN-PROBE INCORPORATEDInventors: Norman C. Nelson, Lyle J. Arnold, Jr., Lizhong Dai, Steven Phelps, Jijumon Chelliserry
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Publication number: 20210403982Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.Type: ApplicationFiled: May 24, 2021Publication date: December 30, 2021Inventors: Norman C. NELSON, Lyle J. ARNOLD, Jr., Lizhong DAI, Steven PHELPS, Jijumon CHELLISERRY
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Patent number: 10724085Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.Type: GrantFiled: February 22, 2018Date of Patent: July 28, 2020Inventors: Steven T. Brentano, Dmitry Lyakhov, Norman C. Nelson, James D. Carlson, Michael M. Becker, Lyle J. Arnold, Jr.
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Patent number: 10196674Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.Type: GrantFiled: September 16, 2015Date of Patent: February 5, 2019Assignee: GEN-PROBE INCORPORATEDInventors: Norman C. Nelson, Lyle J. Arnold, Jr., Lizhong Dai, Steven Phelps, Jijumon Chelliserry
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Patent number: 10179931Abstract: The present invention provides methods for immobilizing target nucleic acids on a solid support utilizing combinatorial capture probe pairs. These pairs contain first and second capture oligonucleotides that each comprise a target binding region, a capture region and a stem region positioned between the target binding and capture regions. The target binding regions comprise nucleic acid sequences that allow them to hybridize to adjacent regions on the target nucleic acid. The stem regions have nucleic acid sequences that are complementary to each other and the capture regions each comprise a sequence that when positioned adjacent to one another produce a combined nucleic acid sequence that is complementary to a portion of an oligonucleotide bound to a solid support.Type: GrantFiled: July 18, 2017Date of Patent: January 15, 2019Inventor: Lyle J. Arnold, Jr.
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Patent number: 10119163Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.Type: GrantFiled: March 2, 2016Date of Patent: November 6, 2018Assignee: GEN-PROBE INCORPORATEDInventors: Steven T. Brentano, Dmitry Lyakhov, Norman C. Nelson, James D. Carlson, Michael M. Becker, Lyle J. Arnold, Jr.
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Publication number: 20180208981Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.Type: ApplicationFiled: February 22, 2018Publication date: July 26, 2018Inventors: Steven T. BRENTANO, Dmitry LYAKHOV, Norman C. NELSON, James D. CARLSON, Michael M. BECKER, Lyle J. ARNOLD, JR.
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Patent number: 9677135Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.Type: GrantFiled: December 17, 2013Date of Patent: June 13, 2017Assignee: GEN-PROBE INCORPORATEDInventors: Steven T. Brentano, Dmitry Lyakhov, James D. Carlson, Norman C. Nelson, Lyle J. Arnold, Jr., Michael M. Becker
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Publication number: 20160348162Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.Type: ApplicationFiled: March 2, 2016Publication date: December 1, 2016Inventors: Steven T. BRENTANO, Dmitry LYAKHOV, Norman C. NELSON, James D. CARLSON, Michael M. BECKER, Lyle J. ARNOLD, Jr.
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Patent number: 9399796Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.Type: GrantFiled: July 31, 2013Date of Patent: July 26, 2016Assignee: GEN-PROBE INCORPORATEDInventors: Steven T. Brentano, Dmitry Lyakhov, Norman C. Nelson, James D. Carlson, Michael M. Becker, Lyle J. Arnold, Jr.
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Patent number: 9243286Abstract: This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The molecular switch portion of the probe is particularly sensitive to the identity of sequences complementary to the molecular switch. Oligonucleotides containing a molecular switch are applicable to all kinds of hybridization processes. Due to the sensitivity of the switch domain of the oligonucleotide, probes containing a molecular switch are particularly useful in the identification of single point mismatches. More specifically, a portion, but not all, of the oligonucleotide becomes unbound from a mismatched target. The invention further relates to methods of using said oligonucleotides for research reagents, and clinical diagnostics. An exemplary oligonucleotide comprises a first hybridizable domain, a second bridging block domain, and a third binding domain.Type: GrantFiled: January 13, 2014Date of Patent: January 26, 2016Assignee: GEN-PROBE INCORPORATEDInventors: Lyle J. Arnold, Jr., Bob D. Brown
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Publication number: 20160002709Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.Type: ApplicationFiled: September 16, 2015Publication date: January 7, 2016Inventors: Norman C. NELSON, Lyle J. ARNOLD, JR., Lizhong DAI, Steven PHELPS, Jijumon CHELLISERRY
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Patent number: 9169512Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.Type: GrantFiled: July 1, 2010Date of Patent: October 27, 2015Assignee: Gen-Probe IncorporatedInventors: Steven T. Brentano, Dmitry Lyakhov, James D. Carlson, Michael M. Becker, Norman C. Nelson, Lyle J. Arnold, Jr.
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Patent number: 9139870Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.Type: GrantFiled: August 30, 2013Date of Patent: September 22, 2015Assignee: Gen-Probe IncorporatedInventors: Norman C. Nelson, Lyle J. Arnold, Jr., Lizhong Dai, Steven Phelps, Jijumon Chelliserry
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Publication number: 20140193815Abstract: This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The molecular switch portion of the probe is particularly sensitive to the identity of sequences complementary to the molecular switch. Oligonucleotides containing a molecular switch are applicable to all kinds of hybridization processes. Due to the sensitivity of the switch domain of the oligonucleotide, probes containing a molecular switch are particularly useful in the identification of single point mismatches. More specifically, a portion, but not all, of the oligonucleotide becomes unbound from a mismatched target. The invention further relates to methods of using said oligonucleotides for research reagents, and clinical diagnostics. An exemplary oligonucleotide comprises a first hybridizable domain, a second bridging block domain, and a third binding domain.Type: ApplicationFiled: January 13, 2014Publication date: July 10, 2014Applicant: Gen-Probe IncorporatedInventors: Lyle J. Arnold, JR., Bob D. Brown
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Publication number: 20140127700Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.Type: ApplicationFiled: December 17, 2013Publication date: May 8, 2014Applicant: GEN-PROBE INCORPORATEDInventors: Steven T. BRENTANO, Dmitry LYAKHOV, James D. CARLSON, Norman C. NELSON, Lyle J. ARNOLD, JR., Michael M. BECKER
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Patent number: 8679789Abstract: This invention relates to oligonucleotides comprising a molecular switch which may exist in an “open” or “closed” position. The molecular switch portion of the probe is particularly sensitive to the identity of sequences complementary to the molecular switch. Oligonucleotides containing a molecular switch are applicable to all kinds of hybridization processes. Due to the sensitivity of the switch domain of the oligonucleotide, probes containing a molecular switch are particularly useful in the identification of single point mismatches. More specifically, a portion, but not all, of the oligonucleotide becomes unbound from a mismatched target. The invention further relates to methods of using said oligonucleotides for research reagents, and clinical diagnostics. An exemplary oligonucleotide comprises a first hybridizable domain, a second bridging block domain, and a third binding domain.Type: GrantFiled: April 30, 2004Date of Patent: March 25, 2014Assignee: Gen-Probe IncorporatedInventors: Lyle J. Arnold, Jr., Bob D. Brown
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Publication number: 20140066326Abstract: Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.Type: ApplicationFiled: August 30, 2013Publication date: March 6, 2014Applicant: GEN-PROBE INCORPORATEDInventors: Norman C. NELSON, Lyle J. ARNOLD, JR., Lizhong DAI, Steven PHELPS, Jijumon CHELLISERRY
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Patent number: 8642268Abstract: Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.Type: GrantFiled: April 30, 2012Date of Patent: February 4, 2014Assignee: Gen-Probe IncorporatedInventors: Steven T. Brentano, Dmitry Lyakhov, James D. Carlson, Norman C. Nelson, Lyle J. Arnold, Jr., Michael M. Becker
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Publication number: 20130309673Abstract: Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.Type: ApplicationFiled: July 31, 2013Publication date: November 21, 2013Applicant: Gen-Probe IncorporatedInventors: Steven T. BRENTANO, Dmitry LYAKHOV, Norman C. NELSON, James D. CARLSON, Michael M. BECKER, Lyle J. ARNOLD, JR.