Abstract: A luminescence sensor, comprising a non-transparent substrate structure (2) having at least one aperture (5) intended to comprise an analyte and a transparent substrate structure (3), which is arranged to or adjacent said first structure (2). The aperture has a smallest lateral dimension, which is smaller than half the effective wavelength of an excitation radiation, such as light at a wavelength of 700 nm, resulting in an effective wavelength in water of about 538 nm. The transparent structure has a trench (4) with a surface portion provided with ligands with an affinity towards a target molecule. The trench results in that a luminophore attached to the target molecule will be positioned at the entrance surface of the aperture, where the excitation energy is largest.

Abstract: A device for monitoring radiation emitted by luminophores present in an analyte fluid of a wiregrid biosensor. The monitoring device comprises a non-polarized light source (41) for illuminating the wiregrid biosensor for exciting fluorescent labels arranged in the analyte fluid of said biosensor. A detector (71) detects radiation emitted by the labels after excitation. A polarizing filter (53) is arranged in between the transparent substrate and the detector for suppression of background emission radiation from labels positioned outside apertures in the wiregrid, in the analyte fluid.

Abstract: A method of determining a color transformation for images of biological material includes preparing a first set of biological test objects using a first preparation method, and preparing a second set of biological test objects using a second preparation method. Each test object in the second set of test objects corresponding to a counterpart test object in the first set of test objects, the test object and its counterpart being of the same biological type of material. For each test object in the first and second set of test objects, the color of the test object is determined thereby generating a first and second set of colors The method further includes generating a conversion table indicating a mapping between the colors in the first set of colors and the corresponding colors in the second set of colors. The first and second preparation methods include first and second staining methods, respectively.

Abstract: There is provided a method of detecting a presence of a luminophore in a detection volume comprising providing excitation radiation in said detection volume. A luminophore is provided in said detection volume being excitable by said excitation radiation. The luminescent radiation is detected to identify the presence of said luminophore in said detection volume. In one aspect of the invention, said luminophore is selected to emit luminescent radiation having a wavelength in said medium that is larger than twice said smallest dimension; and wherein said luminophore is selected to be excitable by excitation radiation having a wavelength in said medium that is smaller than twice said smallest dimension. Accordingly, luminescent radiation is blocked from entering the detector but for the portion present on an interface of the aperture.

Abstract: There is disclosed a lighting device of which the light sources and/or optical elements are hidden in a first cover state associated with a first light state. This enables an unobtrusive lighting system in the first cover state. In a second cover state associated with a second light state, optical elements, e.g. shutters or beam shaping elements, are switched, induced by the heat or the light flux generated by the light sources, such that the lighting system can function properly.

Abstract: A method of determining a colour transformation for images of biological material comprises—preparing a first set (100) of biological test objects (101-105) using a first preparation method, the set comprising at least one test object;—preparing a second set (130) of biological test objects (131-135) using a second preparation method, each test object (131) in the second set of test objects corresponding to a counterpart test object (101) in the first set of test objects, the test object and its counterpart being of the same biological type of material;—for each test object (101) in the first set of test objects, determining a colour (111) of the test object, thereby generating a first set (110) of colours (111-115);—for each test object (131) in the second set of test objects, determining a colour (121) of the test object, thereby generating a second set (120) of colours (121-125),—generating a conversion table (140) indicating a mapping between the colours in the first set (110) of colours and the correspon

Abstract: A device for monitoring radiation emitted by luminophores present in an analyte fluid of a wiregrid biosensor. The monitoring device comprises a non-polarized light source (41) for illuminating the wiregrid biosensor for exciting fluorescent labels arranged in the analyte fluid of said biosensor. A detector (71) detects radiation emitted by the labels after excitation. A polarizing filter (53) is arranged in between the transparent substrate and the detector for suppression of background emission radiation from labels positioned outside apertures in the wiregrid, in the analyte fluid.

Abstract: There is provided a wave guide comprising: a wave guiding medium, having an index of refraction and provided between first and second wave propagating planar structures at least said first planar structure comprises a plurality of slitted-apertures defining a length axis of the first reflective structure; the slitted apertures constructed and arranged to reflect a R-polarized component of said radiation oriented parallel to said length axis; and wherein said first planar structure is arranged between said wave guiding medium and an adjacent medium having an index of refraction equal or larger than the wave guiding medium. In one aspect of the invention, a waveguide is proposed to limit an excitation region wherein luminophores are excited; substantially independent from the surrounding media of the waveguide. Preferentially, the waveguide is used in a luminescence sensor.

Abstract: There is provided a method of detecting a presence of a luminophore in a detection volume comprising providing excitation radiation in said detection volume. A luminophore is provided in said detection volume being excitable by said excitation radiation. The luminescent radiation is detected to identify the presence of said luminophore in said detection volume. In one aspect of the invention, said luminophore is selected to emit luminescent radiation having a wavelength in said medium that is larger than twice said smallest dimension; and wherein said luminophore is selected to be excitable by excitation radiation having a wavelength in said medium that is smaller than twice said smallest dimension. Accordingly, luminescent radiation is blocked from entering the detector but for the portion present on an interface of the aperture.

Abstract: The invention relates to detection of target molecules in an assay, such as a bio-assay, and in particular to multi-variate detection of target molecules. A detector system is disclosed, the detection system comprising an optical guide element (16) for directing luminescence radiation (7) from an associated sample towards a multivariate element (8), the sample contains probe molecules that specifically binds to target molecules; a multivariate element (8) for spatially separating the luminescence radiation (7; 14; 15) to create a plurality of spectral patterns; and a detector (13) for detecting the intensity of a set of spectral patterns, so as to determine the presence of binding complexes between probe molecules and target molecules in the sample.

Abstract: A system (40) for diagnosis and staging of early stages of cancer in the tissue of a patient is provided. The system is configured to combine information from a Polarized Light Scattering Spectroscopy measurement (70) having a first probe depth, and a Differential Path Length Spectroscopy measurement (60) having a second probe depth, wherein the second probe depth is set larger than' the first probe depth. By comparing the results of the Polarized Light Scattering Spectroscopy and Differential Path Length Spectroscopy measurements early stages of cancer, such as dysplasia may be detected. Also hyperplasia, carcinoma in situ, and carcinoma may be detected. A computer-readable medium, method and use are also provided.

Abstract: A luminescence sensor, comprising a non-transparent substrate structure (2) having at least one aperture (5) intended to comprise an analyte and a transparent substrate structure (3), which is arranged to or adjacent said first structure (2). The aperture has a smallest lateral dimension, which is smaller than half the effective wavelength of an excitation radiation, such as light at a wavelength of 700 nm, resulting in an effective wavelength in water of about 538 nm. The transparent structure has a trench (4) with a surface portion provided with ligands with an affinity towards a target molecule. The trench results in that a luminophore attached to the target molecule will be positioned at the entrance surface of the aperture, where the excitation energy is largest.