Patents by Inventor Magda Anastassova Dineva
Magda Anastassova Dineva has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11447821Abstract: Methods for amplifying a target nucleic acid by self-sustained amplification methods are described. The methods are designed, in particular, to be carried out without use of specialised lab facilities or instruments. Compositions, lyophilised formulations, and kits for carrying out the methods are also described.Type: GrantFiled: January 24, 2020Date of Patent: September 20, 2022Assignee: CAMBRIDGE ENTERPRISE LIMITEDInventors: Helen Hwai-an Lee, Magda Anastassova Dineva, Fiona Frances Sarah Fletcher-Brown
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Publication number: 20200157617Abstract: Methods for amplifying a target nucleic acid by self-sustained amplification methods are described. The methods are designed, in particular, to be carried out without use of specialised lab facilities or instruments. Compositions, lyophilised formulations, and kits for carrying out the methods are also described.Type: ApplicationFiled: January 24, 2020Publication date: May 21, 2020Applicant: CAMBRIDGE ENTERPRISE LIMITEDInventors: Helen Hwai-an Lee, Magda Anastassova Dineva, Fiona Frances Sarah Fletcher-Brown
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Patent number: 10563254Abstract: Methods for amplifying a target nucleic acid by self-sustained amplification methods are described. The methods are designed, in particular, to be carried out without use of specialised lab facilities or instruments. Compositions, lyophilised formulations, and kits for carrying out the methods are also described.Type: GrantFiled: January 23, 2008Date of Patent: February 18, 2020Assignee: CAMBRIDGE ENTERPRISE LIMITEDInventors: Helen Hwai-an Lee, Magda Anastassova Dineva, Fiona Frances Sarah Fletcher-Brown
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Patent number: 10315195Abstract: A device for the processing of a sample comprises a location apparatus, a processing chamber for receiving the sample and a plurality of reagent chambers. The reagent chambers have openings defined in the location apparatus. The processing chamber is movable relative to the reagent chambers to enable sequential communication with each reagent chamber in turn.Type: GrantFiled: October 13, 2017Date of Patent: June 11, 2019Assignees: Diagnostics for the Real World, Ltd., Cambridge Enterprise LimitedInventors: Helen Hwai-an Lee, Magda Anastassova Dineva, Craig Alan Wisniewski, Phillip John Stankus
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Publication number: 20180036726Abstract: A device for the processing of a sample comprises a location apparatus, a processing chamber for receiving the sample and a plurality of reagent chambers. The reagent chambers have openings defined in the location apparatus. The processing chamber is movable relative to the reagent chambers to enable sequential communication with each reagent chamber in turn.Type: ApplicationFiled: October 13, 2017Publication date: February 8, 2018Inventors: Helen Hwai-an Lee, Magda Anastassova Dineva, Craig Alan Wisniewski, Phillip John Stankus
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Patent number: 9839909Abstract: A device for the processing of a sample comprises a location apparatus, a processing chamber for receiving the sample and a plurality of reagent chambers. The reagent chambers have openings defined in the location apparatus. The processing chamber is movable relative to the reagent chambers to enable sequential communication with each reagent chamber in turn.Type: GrantFiled: July 27, 2007Date of Patent: December 12, 2017Assignees: Diagnostics for the Real World, Ltd., Cambridge Enterprise LimitedInventors: Helen Hwai-an Lee, Magda Anastassova Dineva, Craig Alan Wisniewski, Phillip John Stankus
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Patent number: 9494584Abstract: Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.Type: GrantFiled: June 21, 2011Date of Patent: November 15, 2016Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Ling Huang, Magda Anastassova Dineva, Hsiang Yun Hu
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Publication number: 20150099265Abstract: Improved dipstick assays for testing for the presence of a target nucleic acid in a sample solution are described. A dipstick is provided which comprises a contact end for contacting the sample solution and a capture zone remote from the contact end for capturing target nucleic acid. Sample solution is contacted with the contact end to cause sample solution to move by capillary action to the capture zone. Target nucleic acid in the sample solution is captured at the capture zone and is detected by a plurality of different labelled detection probes each capable of hybridizing to a different region of the target nucleic acid. The detection signal is thereby enhanced. In other methods a plurality of different capture probes are added to the sample solution which can then be bound by a capture moiety at the capture zone to indirectly capture target nucleic acid. Capture of target nucleic acid is thereby improved. Kits and dipsticks for carrying out such methods are also described.Type: ApplicationFiled: December 19, 2013Publication date: April 9, 2015Applicant: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Magda Anastassova Dineva
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Patent number: 8431336Abstract: Use of dipsticks to test for the presence of target nucleic acid in a sample solution is described. The dipsticks comprise a contact end for contacting the sample solution and a capture zone, remote from the contact end, to which a capture probe is immobilized. The capture probe is capable of hybridising to the target nucleic acid. The sample solution is contacted with the contact end of the dipstick and travels by capillary action to the capture zone. If target nucleic acid is present in the sample solution it is captured and can be detected at the capture zone. The capture probe is immobilized to the capture zone by a spacer. Use of the spacer increases the stability of the interaction between the capture probe and the target nucleic acid and thus improves the sensitivity of target nucleic acid detection. Detection probes with spacers are also described.Type: GrantFiled: July 6, 2001Date of Patent: April 30, 2013Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Magda Anastassova Dineva, Hsiang Yun Hu
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Publication number: 20120094272Abstract: Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.Type: ApplicationFiled: June 21, 2011Publication date: April 19, 2012Applicant: DIAGNOSTICS FOR THE REAL WORLD, LTD.Inventors: Helen Lee, Ling Huang, Magda Anastassova Dineva, Hsiang Yun Hu
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Patent number: 7972837Abstract: Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.Type: GrantFiled: March 5, 2008Date of Patent: July 5, 2011Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Ling Huang, Magda Anastassova Dineva, Hsiang Yun Hu
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Patent number: 7867706Abstract: Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is contacted with the contact end of the dipstick to cause the sample solution to move by capillary action to a capture zone of the dipstick at which target nucleic acid is captured. The target nucleic acid may be captured at the capture zone by a capture probe capable of hybridising to the target nucleic acid. A labelled detection probe capable of hybridising to the target nucleic acid may be used to detect the target nucleic acid at the capture zone. A helper probe may be used to enhance the binding of the capture and/or detection probe to the target nucleic acid, thereby improving the sensitivity of target nucleic acid detection. Dipsticks and kits are also described.Type: GrantFiled: January 19, 2007Date of Patent: January 11, 2011Assignee: Diagnostics For The Real World, Ltd.Inventors: Helen Lee, Magda Anastassova Dineva, Shaun Christopher Hazlewood
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Patent number: 7776617Abstract: Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.Type: GrantFiled: November 30, 2001Date of Patent: August 17, 2010Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Ling Huang, Magda Anastassova Dineva, Hsiang Yun Hu
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Publication number: 20100136542Abstract: Methods for amplifying a target nucleic acid by self-sustained amplification methods are described. The methods are designed, in particular, to be carried out without use of specialised lab facilities or instruments. Compositions, lyophilised formulations, and kits for carrying out the methods are also described.Type: ApplicationFiled: January 23, 2008Publication date: June 3, 2010Applicant: CAMBRIDGE ENTERPRISE LIMITEDInventors: Helen Hwai-an Lee, Magda Anastassova Dineva, Fiona Frances Sarah Fletcher-Brown
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Patent number: 7713746Abstract: The invention provides a dipstick and a kit comprising the dipstick, for testing for the presence of a plurality of different targets in a sample solution which comprises: a dipstick having a plurality of different capture zones and, immobilised to each capture zone, a different capture moiety, each capture moiety capable of capturing a different target; and, separately, a plurality of different detection probes, each detection probe capable of binding to a different target and each detection probe being labelled with or enabling the formation of a detection signal so that the presence of each target is indicated by the formation of a signal at the capture zone for that target; wherein the target for at least two of the capture moieties is a disease causing micro-organism or a marker indicating the existence of a disease, disorder, or condition of the host from which the sample solution was derived, and wherein at least two of the capture moieties are capable of binding to different components or markers of tType: GrantFiled: October 15, 2001Date of Patent: May 11, 2010Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Magda Anastassova Dineva
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Publication number: 20080206853Abstract: Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.Type: ApplicationFiled: March 5, 2008Publication date: August 28, 2008Applicant: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Ling Huang, Magda Anastassova Dineva, Hsiang Yun Hu
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Publication number: 20080160516Abstract: Dipsticks for testing for the presence of a target nucleic acid in a sample solution are described the dipsticks comprise a universal capture probe immobilised at a capture zone of the dipstick. The universal capture probe is capable of hybridising to a hook capture probe which is hybridised to the target nucleic acid in the sample solution. A contact end of the dipstick is contacted with the sample solution to cause hook capture probe hybridised to the target nucleic acid to move by capillary action to the capture zone where the target nucleic acid can be detected. Use of the universal and hook capture probes allows dipsticks to be prepared which can be used to capture any target nucleic acid, thereby simplifying preparation of the dipsticks. Specificity of target nucleic acid capture is then achieved by use of an appropriate hook capture probe. Methods and kits are also described.Type: ApplicationFiled: March 15, 2007Publication date: July 3, 2008Applicant: Diagnostics for the Real World, Ltd.Inventors: Helen LEE, Hsiang Yun Hu, Magda Anastassova Dineva
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Patent number: 7192701Abstract: Dipsticks for testing for the presence of a target nucleic acid in a sample solution are described the dipsticks comprise a universal capture probe immobilised at a capture zone of the dipstick. The universal capture probe is capable of hybridising to a hook capture probe which is hybridised to the target nucleic acid in the sample solution. A contact end of the dipstick is contacted with the sample solution to cause hook capture probe hybridised to the target nucleic acid to move by capillary action to the capture zone where the target nucleic acid can be detected. Use of the universal and hook capture probes allows dipsticks to be prepared which can be used to capture any target nucleic acid, thereby simplifying preparation of the dipsticks. Specificity of target nucleic acid capture is then achieved by use of an appropriate hook capture probe. Methods and kits are also described.Type: GrantFiled: July 6, 2001Date of Patent: March 20, 2007Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Hsiang Yun Hu, Magda Anastassova Dineva
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Patent number: 7186508Abstract: Use of helper probes in dipstick assays is described. In a dipstick assay to test for the presence of a target nucleic acid in a sample solution, the sample solution is connected with the contact end of the dipstick to cause the sample solution is contacted with the contact end of the dipstick to cause the sample solution to move by capillary action to a capture zone of the dipstick at which target nucleic acid is captured. The target nucleic acid may be captured at the capture zone by a capture probe capable of hybridising to the target nucleic acid. A labelled detection probe capable of hybridising to the target nucleic acid may be used to detect the target nucleic acid at the capture zone. A helper probe may be used to enhance the binding of the capture and/or detection probe to the target nucleic acid, thereby improving the sensitivity of target nucleic acid detection. Dipsticks and kits are also described.Type: GrantFiled: July 6, 2001Date of Patent: March 6, 2007Assignee: Diagnostics for the Real World, Ltd.Inventors: Helen Lee, Magda Anastassova Dineva, Shaun Christopher Hazlewood
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Publication number: 20040072176Abstract: Use of dipsticks to test for the presence of target nucleic acid in a sample solution is described. The dipsticks comprise a contact end for contacting the sample solution and a capture zone, remote from the contact end, to which a capture probe is immobilised. The capture probe is capable of hybridising to the target nucleic acid. The sample solution is contacted with the contact end of the dipstick and travels by capillary action to the capture zone. If target nucleic acid is present in the sample solution it is captured and can be detected at the capture zone. The capture probe is immobilised to the capture zone by a spacer. Use of the spacer increases the stability of the interaction between the capture probe and the target nucleic acid and thus improves the sensitivity of target nucleic acid detection. Detection probes with spacers are also described.Type: ApplicationFiled: April 7, 2003Publication date: April 15, 2004Inventors: Helen Lee, Magda Anastassova Dineva, Hsiang Yun Hu