Abstract: Microfluidic devices and methods for analyzing glycan profiles of glycoproteins are provided. Some embodiments of the devices comprise a deglycosylation column for cleaving glycans, an optional cleaning column for removing proteins, a trapping column for enriching glycans, and a separation column for resolving glycans. The devices and methods significantly improve the speed and sensitivity of glycan analysis.

Abstract: Microfluidic devices designed for assaying biochemical molecules are disclosed. The microfluidic devices are capable of assaying nucleic acids for identification of nucleic acid species. The microfluidic devices are adapted to carry out an amplification of the nucleic acid and subsequent separation of amplified nucleic acid species. Also disclosed is a method for amplifying and separating a nucleic acid sample on a microfluidic device.

Abstract: The microfluidic reverse affinity-blot device of the present disclosure combines affinity binding for isolation and/or enrichment of protein(s) from a sample, followed by separation/identification thereof. In general terms, a microfluidic reverse affinity-blot device is a closed system of interconnected components that is comprised of defined points of entry and exit, wherein the interconnected components include a capture region upstream from a protein separation region and subsequent detection region. Methods of use are also described.

Abstract: A microfluidic device for separating a fluid sample into components is disclosed. The microfluidic device is used for assaying proteins for the purpose of identification of protein species. The microfluidic device employs integrated features such as a sample well for holding a protein sample, a thermal control device for heating the protein sample in the sample well, and a protein separation region in fluid communication with the sample well. Also disclosed is a method for assaying a protein sample.

Abstract: The present invention provides methods of detecting and/or isolating immune complexes. The antigen in the immune complexes can further be isolated from the immune complexes to facilitate research and characterization of the antigen. Other embodiments of the present invention can be used to, inter alia, develop assays for detecting immune complexes, diagnose diseases or medical conditions, as well as determine the correlation between the presence of immune complexes and diseases and medical conditions of interest. Still other embodiments provide reagents, equipments and kits for implementing the methods.

Abstract: An assay method is provided. In certain embodiments, the method includes: a) contacting a sample with a polypeptide that is linked to a surface of a surface plasmon resonance sensor under kinasing conditions to produce a sample-contacted substrate; b) separating the sample and the sample-contacted substrate to produce a sample-separated substrate; and c) assessing phosphorylation of the polypeptide using surface plasmon resonance. Kits and systems for performing the instant methods are also provided.

Abstract: Data from kinetic surface plasmon resonance measurements are fitted to time-profile curves. Time profile curves with sub-threshold curvature are flagged as being insufficient to provide reliable determinations of kinetic parameters and binding affinity. The remaining curves are used to calculate the kinetic parameters and binding affinity, obviating the need for multiple runs for these purposes. The resulting increase in throughput enables drug discovery methods in which kinetic measurements are performed earlier in the screening processes, e.g., so that all or most primary-screen hits can be subjected to data-rich kinetic measurements for screening purposes.

Abstract: The invention provides methods of analyzing a sample. In general, the methods involve: a) contacting a sample with an array of capture agents; and b) reading the array to detect the presence of a post-translational modification indicator and an analyte-detection moiety. The analytes of the sample may be contacted with the post-translational modification indicator and analyte-detection moiety at any point in the subject methods. Also provided is a system and kit for performing the subject methods.

Abstract: The invention provides a method of evaluating a feature on a polypeptide array. In general, the method involves reading a polypeptide array under intrinsic fluorescence-detecting conditions to produce data and assessing the data to evaluate the the feature. The invention finds use in a variety of medical and research applications.

Abstract: The present invention provides an array hybridization apparatus including a gasket slide and an array slide. The array slide is adapted to be disposed closely adjacent the gasket slide to form a hybridization chamber for retaining an analyte solution in close proximity to an array. At least one of the gasket slide and the array slide has a disassembly feature which aids in disassembling the hybridization chamber. The invention also provides a method for disassembling an array hybridization chamber.

Abstract: An array hybridization apparatus and method. The apparatus employs a first substrate that contacts and attaches to an array slide, a second substrate that contacts and attaches to a gasket slide, and a separation means for separating the first substrate from the second substrate. Methods of disassembly of the apparatus are also disclosed.

Abstract: The invention provides a method of producing a protein bioarray that includes providing a substrate comprising a solid support and a surface modification layer bound to the solid support. The surface modification layer includes a first moiety having the structure —Si—R1 and a second moiety having the structure —Si-L-R2, wherein R1 is a chemically inert moiety selected from the group consisting of C3 to C30 alkyl and benzyl optionally substituted with 1 to 5 halogen atoms, L is a linking group, and R2 is a chemically inert hydrophilic moiety. The method of producing the protein bioarray further includes providing at least two solutions, wherein each solution contains a probe protein. In the method, each of the solutions is then deposited at its own discrete site on the substrate. The probe proteins deposited on the substrate become non-covalently bound to the substrate.

Abstract: A multi-dimensional chemical-analysis system includes a longitudinally extending iso-electric focusing (IEF) channel and transversely extending high-pressure liquid chromatography (HPLC) channels. Piezo-electric pumps force sample fluid from the IEF channel to form jets as it exits through respective pump nozzles. The jets then enter the HPLC channels for parallel second-dimension separations. Alternatively, the jets can be used to “print” sample onto a moving medium to provide a two-dimensional time-vs.-band-position distribution of sample components.