Abstract: The invention discloses a method for sanitization of a bioprocess chro matography column, comprising the steps of: a) providing a bioprocess chromatogra phy column with a packed bed of separation resin particles and a movable adapter, b) with the adapter in contact with the packed bed, conveying a sanitization fluid through the packed bed to a column outlet; c) raising the adapter to provide a gap between the packed bed and the adapter, d) lowering the adapter to close the gap; and e) conveying an equilibration liquid through the packed bed to a column outlet.

Abstract: A method for operating a real-time-capable simulation network having multiple network nodes for computing a simulation model. The network nodes are connected to one another via a serial data bus, and the network nodes exchange data via data bus messages. At least one event-driven task of the simulation model is implemented on a first network node, and a nondeterministic triggering event is detected by a second network node. The second network node communicates the detected triggering event to the first network node and the first network node computes the event-driven task. A fast response time is achieved by the means that a detection signal is sent from the second network node in the form of a multicast data bus message or a broadcast data bus message to multiple network nodes of the simulation network or to all network nodes of the simulation network over the serial data bus.

Abstract: The invention discloses a method for individually assessing the cleanness of at least one of an upper (2) and a lower (3) bed support in a process column (1). The method comprises the steps of: a) providing a process column (1) comprising an upper (2) and a lower (3) bed support, a column chamber, a movable adaptor (5), an upper outlet (6) and a lower outlet (7); b) conveying liquid through one of the lower (3) and upper (2) bed supports, e.g. by moving the adaptor (5) downwards at a predetermined rate with one of the upper and lower outlets closed and the other open to force liquid through one of the lower and upper bed supports, and; c) measuring the differential pressure over the bed support through which liquid passes.

Abstract: The invention discloses a method for individually assessing the cleanness of at least one of an upper (2) and a lower (3) bed support in a process column (1). The method comprises the steps of: a) providing a process column (1) comprising an upper (2) and a lower (3) bed support, a column chamber, a movable adaptor (5), an upper outlet (6) and a lower outlet (7); b) conveying liquid through one of the lower (3) and upper (2) bed supports, e.g. by moving the adaptor (5) downwards at a predetermined rate with one of the upper and lower outlets closed and the other open to force liquid through one of the lower and upper bed supports, and; c) measuring the differential pressure over the bed support through which liquid passes.

Abstract: A tank adapted to be used for suspending separation media slurry before transferring the separation media slurry to a column or a container comprises: an impeller (7) adapted to rotate in order to mix the media slurry, a nozzle (9) provided protruding into the tank from the bottom (8) thereof, and an adjustable pipe (5) having an open end (6) positioned in the tank and which can be adjusted in position. The nozzle (9) and the adjustable pipe (5) are adapted to be connected to a pump (3) adapted to pump liquid from the adjustable pipe (5) when the open end (6) of the adjustable pipe (5) has been positioned in an uppermost liquid phase provided in the tank when the media slurry has started to sediment and pump the liquid, possibly containing a small amount of media, through the nozzle (9) back into the tank from the tank bottom (8) in order to start re-suspending media that has sedimented in the tank.

Abstract: A tank adapted to be used for suspending separation media slurry before transferring the separation media slurry to a column or a container comprises: an impeller (7) adapted to rotate in order to mix the media slurry, a nozzle (9) provided protruding into the tank from the bottom (8) thereof, and an adjustable pipe (5) having an open end (6) positioned in the tank and which can be adjusted in position. The nozzle (9) and the adjustable pipe (5) are adapted to be connected to a pump (3) adapted to pump liquid from the adjustable pipe (5) when the open end (6) of the adjustable pipe (5) has been positioned in an uppermost liquid phase provided in the tank when the media slurry has started to sediment and pump the liquid, possibly containing a small amount of media, through the nozzle (9) back into the tank from the tank bottom (8) in order to start re-suspending media that has sedimented in the tank.

Abstract: A method for synchronously providing data on distributed devices of a network includes storing, by a master device, an extrapolation data set including at least one update time point. The update time point is in the future and marks the beginning of an extrapolation interval. The master device transmits the extrapolation data set to a slave device. Starting at an update time point, new data is calculated separately on the master device and the slave device by extrapolation using the extrapolation data set available on both the master device and the slave device. The method steps are repeated for subsequent extrapolation intervals.

Abstract: A method for synchronously providing data on distributed devices of a network includes storing, by a master device, an extrapolation data set including at least one update time point. The update time point is in the future and marks the beginning of an extrapolation interval. The master device transmits the extrapolation data set to a slave device. Starting at an update time point, new data is calculated separately on the master device and the slave device by extrapolation using the extrapolation data set available on both the master device and the slave device. The method steps are repeated for subsequent extrapolation intervals.