Abstract: A microfluidic passive sample separation device for passively separating a liquid sample, and optionally detecting an analyte in the separated liquid sample. The device includes a filter for filtering the liquid sample and for producing a filtrate; a plurality of capillaries configured to withdraw the filtrate from the filter by capillary force, a capillary micropump for receiving the filtrate and pumping the filtrate to the outlet.

Abstract: Microneedles for detecting targets are described. The microneedles may be made of a hydrogel and a probe coupled to the hydrogel for generating a measurable signal in the presence of the target. The hydrogel microneedles may be used for in-situ detection of targets, such as biomolecules found in interstitial fluid. Also described are methods or producing hydrogel microneedles, articles and apparatus comprising hydrogel microneedles, and methods and uses of the same. The hydrogel microneedles may be used for biosensing, such as in transdermal patches for detecting biomarkers in a subject. The biosensors may be used for continuous, real-time tracking of targets in-situ, without requiring further reagents or processing steps.

Abstract: Microneedle electrodes for detecting targets or sensing pH are described. The microneedles may be made of a hydrogel, a probe coupled to the hydrogel for generating an electrochemical signal in the presence of the target, and a conductive material for communicating the electrochemical signal through the hydrogel. The hydrogel microneedles may be used for in-situ detection of targets, such as biomolecules found in interstitial fluid. Also described are methods of producing hydrogel microneedles, articles and apparatus comprising microneedle electrodes, and methods and uses of the same. The microneedle electrodes may be used for biosensing, such as in transdermal patches for detecting biomarkers in a subject. The biosensors may be used for continuous, real-time tracking of targets in-situ, without requiring further reagents or processing steps.

Abstract: The present disclosure provides materials and methods for the continuous measurement of biomolecules in vivo and in real-time. The present disclosure relates more specifically to using capture agents and detection agents within a microfluidic device to detect and quantify biochemical features of biomarkers, enabling real-time detection and concentration measurements.

Abstract: Methods and devices for magnetic profiling of target particles in a flow. There are a plurality of flow rate-reducing structures in a flow chamber. Each flow rate-reducing structure is provided with a localized magnetic attractive force, the magnetic attractive force defining a capture zone in the vicinity of the flow rate-reducing structure. The size of capture zones may be variable for different locations within the device. The magnetic attractive force, in the capture zone, is sufficiently high to overcome the drag force on a given subset of the target particles to promote capture of any particles belonging to the subset of the target particles in the capture zone. Different target particles having different magnetic susceptibility are captured in different capture zones.

Abstract: Methods and devices for magnetic profiling of target particles in a flow. There are a plurality of flow rate-reducing structures in a flow chamber. Each flow rate-reducing structure is provided with a localized magnetic attractive force, the magnetic attractive force defining a capture zone in the vicinity of the flow rate-reducing structure. The size of capture zones may be variable for different locations within the device. The magnetic attractive force, in the capture zone, is sufficiently high to overcome the drag force on a given subset of the target particles to promote capture of any particles belonging to the subset of the target particles in the capture zone. Different target particles having different magnetic susceptibility are captured in different capture zones.