Patents by Inventor Maiko Tanabe
Maiko Tanabe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11926845Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.Type: GrantFiled: January 29, 2021Date of Patent: March 12, 2024Assignee: Hitachi, Ltd.Inventors: Yuki Niimi, Hiroko Hanzawa, Maiko Tanabe, Kunio Ohyama, Shizu Takeda
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Patent number: 11680286Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.Type: GrantFiled: May 18, 2021Date of Patent: June 20, 2023Assignee: Hitachi, Ltd.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe
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Patent number: 11566232Abstract: The purpose of the invention is to provide novel cell culture substrates, cell culture vessels, and methods for cell culture. A cell culture substrate having a planar mesh structure, the substrate being coated with a polymer, is provided. Cells are cultured in a cell culture vessel having this substrate.Type: GrantFiled: September 6, 2018Date of Patent: January 31, 2023Assignees: HITACHI, LTD., THE UNIVERSITY OF TOKYOInventors: Maiko Tanabe, Shizu Takeda, Masahiro Okanojo, Hiroko Hanzawa, Masao Washizu, Kennedy Omondi Okeyo
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Patent number: 11446963Abstract: A tyre includes a tread portion including a first middle land portion, a second middle land portion, a first shoulder land portion, and a second shoulder land portion. The first middle land portion is provided with first middle lateral grooves terminating within the first middle land portion, the second middle land portion is provided with second middle lateral grooves terminating within the second middle land portion, the first shoulder land portion is provided with first shoulder lateral grooves, and the second shoulder land portion is provided with second shoulder lateral grooves. The first middle lateral grooves and the second middle lateral grooves are inclined in a first direction with respect to the tyre axial direction, and the first shoulder lateral grooves and the second shoulder lateral grooves are inclined in a second direction opposite to the first direction with respect to the tyre axial direction.Type: GrantFiled: September 16, 2019Date of Patent: September 20, 2022Assignee: Sumitomo Rubber Industries, Ltd.Inventors: Maiko Tanabe, Ryo Oba, Tetsuya Maekawa
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Patent number: 11312183Abstract: A tyre includes a tread portion having an outer shoulder main groove, a crown main groove, and an outer middle land region defined between the outer shoulder main groove and the crown main groove. The outer middle land region is provided with first middle sipes each extending inwardly in a tyre axial direction from the outer shoulder main groove to have an inner end in the outer middle land region and second middle sipes each extending outwardly in the tyre axial direction from the crown main groove to have an outer end in the outer middle land region. The number of the second middle sipes is larger than the number of the first middle sipes.Type: GrantFiled: May 16, 2019Date of Patent: April 26, 2022Assignee: SUMITOMO RUBBER INDUSTRIES, LTD.Inventors: Maiko Tanabe, Ryuta Kitora
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Publication number: 20210388422Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.Type: ApplicationFiled: May 18, 2021Publication date: December 16, 2021Inventors: Masataka SHIRAI, Hideki KAMBARA, Kiyomi TANIGUCHI, Maiko TANABE
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Publication number: 20210284959Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.Type: ApplicationFiled: January 29, 2021Publication date: September 16, 2021Inventors: Yuki NIIMI, Hiroko HANZAWA, Maiko TANABE, Kunio OHYAMA, Shizu TAKEDA
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Patent number: 11053536Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.Type: GrantFiled: July 30, 2012Date of Patent: July 6, 2021Assignee: Hitachi, Ltd.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe
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Patent number: 10876106Abstract: In order to interpret an arbitrary sequence region in many genes in many cells, it is necessary to degrade a nucleic acid into fragments and introduce a sequence that is different from one cell to another into each of the fragments. However, in the conventional configuration for analyzing many cells, there has been a problem that mixing of the degraded fragments among areas occurs before a tag sequence unique for each of the areas is introduced. The present invention provides a system for capturing a nucleic acid extracted from a cell in each of plural areas on a substrate and synthesizing a complementary DNA (cDNA) of the nucleic acid for each of the areas, wherein the system also includes a means for immediately introducing a tag sequence unique for each of the areas to the reaction product.Type: GrantFiled: April 9, 2015Date of Patent: December 29, 2020Assignee: HITACHI, LTD.Inventors: Maiko Tanabe, Masataka Shirai, Tomoyuki Sakai
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Publication number: 20200327958Abstract: A method for making a recombinant gene includes searching a database using a nucleotide sequence of a coding region, a nucleotide sequence that encodes an amino acid sequence, or an amino acid sequence, of a gene, for one or more nucleotide sequences having homology; selecting one or more nucleotide sequences other than nucleotide sequences only derived from a genome from the selected nucleotide sequences; for ones of the selected one or more nucleotide sequences comprising an upstream or downstream nucleotide sequence, analyzing whether the upstream or downstream nucleotide sequence is a functional sequence to select one or more first functional sequences; for ones of the selected one or more nucleotide sequences comprising no upstream or downstream nucleotide sequence, analyzing whether a gene information has any description indicating a functional sequence to select one or more second functional sequences; scoring the selected functional sequences; and selecting one or more functional sequences.Type: ApplicationFiled: March 19, 2020Publication date: October 15, 2020Inventors: Maiko TANABE, Shizu TAKEDA, Kiyoto ITO, Osamu IMAICHI, Kenji TSUGE, Michihiro ARAKI
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Publication number: 20200303042Abstract: To predict a new biological reaction by quantifying while retaining characteristics of an entire compound structure. To provide a structural characteristic amount encoding unit that includes a conversion model unit configured to convert a characteristic amount of notation information indicating chemical structures of a plurality of compounds into a dispersedly represented numerical vector having at least two or more real number values as an element using a conversion parameter, the conversion model unit converting the characteristic amount of the notation information indicating the chemical structures into a numerical vector, for each of a first compound and a second compound among the plurality of compounds, and a biological reaction characteristic vector generator configured to generate a biological reaction characteristic vector between the first compound and the second compound by performing a calculation using a numerical vector of the first compound and a numerical vector of the second compound.Type: ApplicationFiled: March 12, 2020Publication date: September 24, 2020Inventors: Taiki FUJI, Kiyoto ITO, Shiori NAKAZAWA, Maiko TANABE
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Publication number: 20200114696Abstract: A tyre includes a tread portion including a first middle land portion, a second middle land portion, a first shoulder land portion, and a second shoulder land portion. The first middle land portion is provided with first middle lateral grooves terminating within the first middle land portion, the second middle land portion is provided with second middle lateral grooves terminating within the second middle land portion, the first shoulder land portion is provided with first shoulder lateral grooves, and the second shoulder land portion is provided with second shoulder lateral grooves. The first middle lateral grooves and the second middle lateral grooves are inclined in a first direction with respect to the tyre axial direction, and the first shoulder lateral grooves and the second shoulder lateral grooves are inclined in a second direction opposite to the first direction with respect to the tyre axial direction.Type: ApplicationFiled: September 16, 2019Publication date: April 16, 2020Applicant: Sumitomo Rubber Industries, Ltd.Inventors: Maiko TANABE, Ryo OBA, Tetsuya MAEKAWA
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Publication number: 20190366774Abstract: A tyre includes a tread portion having an outer shoulder main groove, a crown main groove, and an outer middle land region defined between the outer shoulder main groove and the crown main groove. The outer middle land region is provided with first middle sipes each extending inwardly in a tyre axial direction from the outer shoulder main groove to have an inner end in the outer middle land region and second middle sipes each extending outwardly in the tyre axial direction from the crown main groove to have an outer end in the outer middle land region. The number of the second middle sipes is larger than the number of the first middle sipes.Type: ApplicationFiled: May 16, 2019Publication date: December 5, 2019Applicant: Sumitomo Rubber Industries, Ltd.Inventors: Maiko TANABE, Ryuta KITORA
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Publication number: 20190085299Abstract: The purpose of the invention is to provide novel cell culture substrates, cell culture vessels, and methods for cell culture. A cell culture substrate having a planar mesh structure, the substrate being coated with a polymer, is provided. Cells are cultured in a cell culture vessel having this substrate.Type: ApplicationFiled: September 6, 2018Publication date: March 21, 2019Inventors: Maiko TANABE, Shizu TAKEDA, Masahiro OKANOJO, Hiroko HANZAWA, Masao WASHIZU, Kennedy Omondi OKEYO
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Patent number: 10030240Abstract: In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.Type: GrantFiled: March 12, 2013Date of Patent: July 24, 2018Assignee: Hitachi, Ltd.Inventors: Masataka Shirai, Hideki Kambara, Kiyomi Taniguchi, Maiko Tanabe
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Publication number: 20180119135Abstract: In order to interpret an arbitrary sequence region in many genes in many cells, it is necessary to degrade a nucleic acid into fragments and introduce a sequence that is different from one cell to another into each of the fragments. However, in the conventional configuration for analyzing many cells, there has been a problem that mixing of the degraded fragments among areas occurs before a tag sequence unique for each of the areas is introduced. The present invention provides a system for capturing a nucleic acid extracted from a cell in each of plural areas on a substrate and synthesizing a complementary DNA (cDNA) of the nucleic acid for each of the areas, wherein the system also includes a means for immediately introducing a tag sequence unique for each of the areas to the reaction product.Type: ApplicationFiled: April 9, 2015Publication date: May 3, 2018Inventors: Maiko TANABE, Masataka SHIRAI, Tomoyuki SAKAI
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Publication number: 20180073023Abstract: An object of the present invention is to provide a circular single-stranded nucleic acid, and a method for preparing the same and a method for using the same. A circular single-stranded nucleic acid according to one embodiment of the present invention is a circular single-stranded nucleic acid for determining a target base on a genomic DNA, and includes a first single-stranded nucleic acid which has the target base or a complementary base thereto and is a part of one of the strands of the genomic DNA, and a second single-stranded nucleic acid which has an index sequence to serve as an index of a cell, from which the genomic DNA is derived, or a complementary sequence thereto.Type: ApplicationFiled: August 30, 2017Publication date: March 15, 2018Inventors: Hiroko MATSUNAGA, Maiko TANABE
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Patent number: 9862993Abstract: In order to decode arbitrary sequence regions for a large number of genes in a large number of cells, it is necessary to fragment the nucleic acids and introduced a sequence, which differs for each the cell, in the respective fragments. However, in conventional constructions for analyzing large numbers of cells, there was the problem that the cleaved fragments of different regions were intermingled before a tag sequence unique to each region could be introduced. The present invention is constructed to also comprise a genetic analysis system, when trapping nucleic acids extracted from a cell in multiple regions on a substrate and synthesizing and fragmenting the complementary DNA strands (cDNA) of the nucleic acids for each individual region, for immediately introducing a tag sequence unique to each of the regions into said fragments.Type: GrantFiled: October 21, 2013Date of Patent: January 9, 2018Assignee: HITACHI, LTD.Inventors: Maiko Tanabe, Hideki Kambara, Masataka Shirai
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Publication number: 20160251705Abstract: In order to decode arbitrary sequence regions for a large number of genes in a large number of cells, it is necessary to fragment the nucleic acids and introduced a sequence, which differs for each the cell, in the respective fragments. However, in conventional constructions for analyzing large numbers of cells, there was the problem that the cleaved fragments of different regions were intermingled before a tag sequence unique to each region could be introduced. The present invention is constructed to also comprise a means, when trapping nucleic acids extracted from a cell in multiple regions on a substrate and synthesizing and fragmenting the complementary DNA strands (cDNA) of the nucleic acids for each individual region, for immediately introducing a tag sequence unique to each of the regions into said fragments.Type: ApplicationFiled: October 21, 2013Publication date: September 1, 2016Applicant: HITACHI, LTD.Inventors: Maiko Tanabe, Hideki Kambara, Masataka Shirai
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Patent number: 9302548Abstract: A pneumatic tire comprises an asymmetric tread pattern with a designated install direction to a vehicle having an outboard tread edges, the tread pattern having an outboard tread portion between the outboard tread edge and a tire equator and provided with an outer main groove, the outer main groove having a cross section comprising a bottom with a depth of 5.5 to 6.5 mm, and axially inner and outer groove walls each extending from the bottom to a tread contact surface, respectively, at least one of groove walls having a stepwise shape comprising a radially inner portion extending from the bottom with a height of 2.0 to 4.0 mm, an axially extending stepped portion extending from the radially inner portion with a length of 0.75 to 1.25 mm, and a radially outer portion between the stepped portion and the tread contact surface.Type: GrantFiled: August 8, 2013Date of Patent: April 5, 2016Assignee: SUMITOMO RUBBER INDUSTRIES, LTD.Inventors: Maiko Tanabe, Junichiro Morozumi