Abstract: A microfluidic device (100) for mixing a liquid L is provided. The microfluidic device (100) comprises a microfluidic chamber (20), having an inlet (30), and arranged to receive the liquid L therein. In use, the microfluidic device (100) is arranged to control translation through the liquid L of a body B introduced therein, wherein the translation of the body B is due to a potential field acting on the body. In this way, the controlled translation of the body B mixes the liquid L in the microfluidic chamber (20).

Abstract: A fluidic device (10) is described. The fluidic device (10) comprises the first part (110) and the second part (120). The first part (110) comprises a first inlet (111) and a first outlet (112), mutually spaced apart. The second part (120) comprises a first chamber (121) arranged to contain a predetermined first amount A1 of a first fluid F1 therein and a first wall portion (122) arranged to contain, at least in part, the first fluid F1 in the first chamber (121). The fluidic device (10) is arrangeable in a first configuration, wherein the first part (110) is fluidically isolated from the first chamber (121). The fluidic device (10) is arrangeable in a second configuration, wherein the first inlet (111) and the first outlet (112) are fluidically coupled via the first chamber (121), whereby increasing a first pressure P1 in the first chamber (121) via the first inlet (111) urges at least a part of the predetermined first amount A1 of the first fluid F1 through the first outlet (112).

Abstract: A fluidic device (10) is described. The fluidic device (10) comprises the first part (110) and the second part (120). The first part (110) comprises a first inlet (111) and a first outlet (112), mutually spaced apart. The second part (120) comprises a first chamber (121) arranged to contain a predetermined first amount A1 of a first fluid F1 therein and a first wall portion (122) arranged to contain, at least in part, the first fluid F1 in the first chamber (121). The fluidic device (10) is arrangeable in a first configuration, wherein the first part (110) is fluidically isolated from the first chamber (121). The fluidic device (10) is arrangeable in a second configuration, wherein the first inlet (111) and the first outlet (112) are fluidically coupled via the first chamber (121), whereby increasing a first pressure P1 in the first chamber (121) via the first inlet (111) urges at least a part of the predetermined first amount A1 of the first fluid F1 through the first outlet (112).

Abstract: A microfluidic device (200) for separating a liquid L into first and second liquid components L1, L2 thereof is described. The microfluidic device (200) comprises an inlet (230) for receiving the liquid therethrough. The microfluidic device (200) comprises a first outlet (210) for the first liquid component L1, wherein the first outlet (210) is fluidically coupled to the inlet (230) via a first passageway (240). The microfluidic device (200) comprises a second outlet (220) for the second liquid component L2, wherein the second outlet (220) is fluidically coupled to the first passageway (240A) via a first set of N conduits 250 (250A, 250B, 250C, 250D, 250E), wherein N is a positive integer greater than 1, wherein respective conduits 250A, 250B, 250C, 250D, 250E of the first set of N conduits 250 divide from the first passageway 240A at respective divisions 252 (252A, 252B, 252C, 252D, 252E) from the inlet 230 therealong 240.

Abstract: A microfluidic device (100) for mixing a liquid L is provided. The microfluidic device (100) comprises a microfluidic chamber (20), having an inlet (30), and arranged to receive the liquid L therein. In use, the microfluidic device (100) is arranged to control translation through the liquid L of a body B introduced therein, wherein the translation of the body B is due to a potential field acting on the body. In this way, the controlled translation of the body B mixes the liquid L in the microfluidic chamber (20).

Abstract: Provided herein are methods for the assembly of a polynucleic acid sequence that is at least partially carried out on a microfluidic device; methods for the preparation of a library of polynucleic acid sequences; microfluidic devices; methods for designing nucleic acid sequences; methods for planning the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences; systems comprising components for carrying out these methods; computer programs which, when run on a computer, implements these methods; and computer readable medium or carrier signals encoding such a computer program.

Abstract: The present invention provides a method for the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences in which the polynucleic acid sequence is of a formula Nn+1, in which N represents a nucleic acid sequence and where n is 1 or greater than 1 and each N may be the same or a different nucleic acid sequence, in which the method comprises: (i) providing a first nucleic acid sequence N1 which has an oligonucleotide linker sequence L13? at the 3?-end of the nucleic acid sequence; (ii) providing a second nucleic acid sequence N2 which optionally has an oligonucleotide linker sequence L23? at the 3?-end of the nucleic acid sequence and which has an oligonucleotide linker sequence L25? at the 5?-end of the nucleic acid sequence, wherein the 5?-end linker sequence L25? of nucleic acid sequence N2 is complementary to the 3?-end linker sequence L13? of nucleic acid sequence N1; (iii) optionally providing one or more additional nucleic acid sequences N, wherein nucleic acid sequence N2