Abstract: A method for preparing a PRP conjugate having high storage stability and a method for producing a Hib conjugate vaccine are provided. A method for preparing PRP conjugate in accordance with the embodiments of the present invention is characterized by that the release of PRPs after preparing the PRP conjugate is suppressed by using PRP with a lowered molecular weight than native PRP for a coupling reaction between PRP and a carrier protein. Also, a Hib conjugate vaccine comprising as an antigen the PRP conjugate prepared by said method has excellent storage stability and maintain sufficient immunogenicity.

Abstract: A method for preparing a PRP conjugate having high storage stability and a method for producing a Hib conjugate vaccine are provided. A method for preparing PRP conjugate in accordance with the embodiments of the present invention is characterized by that the release of PRPs after preparing the PRP conjugate is suppressed by using PRP with a lowered molecular weight than native PRP for a coupling reaction between PRP and a carrier protein. Also, a Hib conjugate vaccine comprising as an antigen the PRP conjugate prepared by said method has excellent storage stability and maintain sufficient immunogenicity.

Abstract: A method for using inactivated Japanese encephalitis virus particles as an adjuvant of a vaccine is provided. A method for using inactivated Japanese encephalitis virus (JEV) particles as an adjuvant of various vaccines or a mixed vaccine, said JEV particles being obtained by inoculating JEV Beijing-1 strain to Vero cells, culturing said JEV-infected cells to give cultured cells or culture supernatant, purifying JEV particles from said cultured cells or culture supernatant and inactivating said JEV particles with formalin, a method for preparing a (mixed) vaccine which comprises a step of letting inactivated Japanese encephalitis virus be contained, and a mixed vaccine prepared by said method.

Abstract: A method for using inactivated Japanese encephalitis virus particles as an adjuvant of a vaccine is provided. A method for using inactivated Japanese encephalitis virus (JEV) particles as an adjuvant of various vaccines or a mixed vaccine, said JEV particles being obtained by inoculating JEV Beijing-1 strain to Vero cells, culturing said JEV-infected cells to give cultured cells or culture supernatant, purifying JEV particles from said cultured cells or culture supernatant and inactivating said JEV particles with formalin, a method for preparing a (mixed) vaccine which comprises a step of letting inactivated Japanese encephalitis virus be contained, and a mixed vaccine prepared by said method.

Abstract: This invention is related to a heat-shielding method which comprises disposing a thin plate having an IR-reflecting function adjacent to one side of a coated plate and disposing said coated plate in a position such that the surface adjacent to said thin plate is on the side not exposed to infrared light.

Abstract: A method which includes enhancing effects upon treating the surface of a thermoplastic resin article by a surface-activating treatment such as corona discharge and the like or by a primer treatment and, thereby, improving coating performance, printability, adhesiveness and so forth of the thermoplastic resin article. The resin article is subjected to a surface-heating treatment which comprises heating only surface layer of the resin article up to a melting point of the thermoplastic resin or a higher temperature followed by cooling the surface layer, and then, subjected to the surface-activating treatment or primer coating.

Abstract: In a method of corona discharge processing of resin formings surface, excellent corona discharge processing is possible, even if there is a distance between the resin formings and the discharge electrode, by generating a high voltage pulse having a pulse width of 1 .mu.s or more, an average electric field strength of 4 to 20 kv/cm denoted by applied voltage (wave height) versus interelectrode distance, and a pulse frequency of 10 pps or more. This processing is performed by the apparatus which comprises arranging a transfer conveyor to transfer the resin formings, a counter-electrode placed in a down side of the transfer conveyor, a dielectric to cover a surface of the counter-electrode, and a number of discharge electrodes which are arranged in a definite distance above the resin formings along the transfer route.