Abstract: A composition for treatment of HSV-related pathologies including an expression vector for altering expression of a target sequence in an HSV-infected cell by production of single-stranded cDNA (ssDNA) in the cell in vivo suspended for topical application to an affected site in a suitable delivery vehicle. The expression vector is comprised of a cassette comprising a sequence of interest, an inverted tandem repeat, and a primer binding site 3? to the inverted tandem repeat, and a reverse transcriptase/RNAse H coding gene, and is transfected into the infected cells for inhibition of HSV replication. The resulting ssDNA binds to the target sequence to alter expression of the target sequence for such purposes as gene activation or inactivation using duplex or triplex binding of nucleic acids, site-directed mutagenesis, interruption of cellular function by binding to specific cellular proteins, or interfering with RNA splicing functions.

Abstract: The invention provides an improved cell free amplification method capable of producing large quantities of therapeutic-quality nucleic acids and methods of using the synthesized nucleic acid in research, therapeutic and other applications—The methods combine several different state-of-the-art procedures and coordinate their applications to affordably synthesize nucleic acids for therapeutic purposes. It combines in vitro rolling circle amplification, high fidelity polymerases, high affinity primers, and streamlined template specifically designed for particular applications. For expression purposes, the templates contain an expression cassette including a eukaryotic promoter, the coding sequence for the gene of interest, and a eukaryotic termination sequence.

Abstract: A composition for treatment of HSV-related pathologies including an expression vector for altering expression of a target sequence in an HSV-infected cell by production of single-stranded cDNA (ssDNA) in the cell in vivo suspended for topical application to an affected site in a suitable delivery vehicle. The expression vector is comprised of a cassette comprising a sequence of interest, an inverted tandem repeat, and a primer binding site 3? to the inverted tandem repeat, and a reverse transcriptase/RNAse H coding gene, and is transfected into the infected cells for inhibition of HSV replication. The resulting ssDNA binds to the target sequence to alter expression of the target sequence for such purposes as gene activation or inactivation using duplex or triplex binding of nucleic acids, site-directed mutagenesis, interruption of cellular function by binding to specific cellular proteins, or interfering with RNA splicing functions.

Abstract: A pulse generation and tracking system for connection to an object or a subject under treatment therewith, the waveform being a train of repetitive complex pulses each pulse comprising a large amplitude, short duration positive polarity portion and a small amplitude, long duration negative polarity portion, and a zero value portion. The prescribed pulse train for a subject is delivered by a delivery device, which also provides monitoring of the averaged voltage amplitude of the positive portion in terms of either voltage or current value. The impedance of the subject is expected to change over time and cause the individual pulses to vary from predetermined limits. When this occurs, then a subsequent generation of pulses will be changed so that the pulses are again delivered within the predetermined value limits or boundary. Tracking and generation can be operationally crossed, if desired, so that a voltage out of boundary will cause the current of a subsequent pulse to be changed or vice versa.

Abstract: A regularly repetitive, almost periodic but non-regularly shaped waveform is amplitude sampled at an interval different from that of the waveform under investigation. For a voltage measurement, the positive samples are averaged for a predetermined count number (one set) and the negative samples are likewise averaged. Zero value samples are ignored. The highest absolute value of the two is taken as a meaningful measurement. Alternatively, one of these values can be selected. For a current measurement, a running average for a predetermined number of sets can also be developed. Finally, frequency can be developed by sensing the transitions and operating a counter on/off at the beginning and ending of a waveform cycle. The count of regular interval counts is then multiplied by the interval time to give the period of the waveform. The reciprocal of the period is a measure of the frequency.