Abstract: The present invention provides a method for production of a spread, comprising the steps of:(a) two aqueous phases with different particle sizes in a fatty phase, of which at least one aqueous phase is dispersed into the fatty phase through a microporous membrane, to prepare a mixed water-in-oil type emulsion;(b) rapidly cooling the emulsion to plasticize; and(c) kneading the plasticized emulsion.According to the present invention, it is possible to produce a low-fat spread having good taste easily, which does not require a stabilizer or a gelling agent, is excellent in stability and preservativity on a level never achieved by any conventional methods.

Abstract: An ice cream having a property of excellent meltdown in the mouth, a fat content of 5 to 18% by weight and an overrun of 10 to 150% by weight, containing air cells of 60 .mu.m or more in diameter at a ratio of at least 80% of the total air cells, is disclosed. Further, a process for producing an ice cream having a property of excellent softness even at a freezing temperature, which comprises incorporating air into an ice cream mix in a first cylinder of the cylinders of two linked continuous freezers to give an overrun, transferring the mix to a second cylinder at a specific temperature, and then mixing and kneading the mix in the second cylinder without giving any additional overrun. In the process for production, an apparatus characterized by having a pipeline linking an apparatus for supplying the rest of an ice cream mix to a pipeline linking the first cylinder and the second cylinder of the cylinders of 2 linked continuous freezers is used.

Abstract: Lactoferrin hydrolyzates, having a decomposition rate between 6%-20% as measured by formol titration, for use as an antibacterial agent, and which have remarkably more potent activity than unhydrolyzed lactoferrin; and lactoferrin hydrolyzates, having a decomposition rate between 4-50% as measured by formol titration, for use as a tyrosinase inhibition agent, are obtainable by conventional methods for hydrolysis with acids or enzymes, and are stable to heating.

Abstract: A method for treatment of a matter which contains moisturized or liquified lactoferrin which has been isolated from mammalian milk, processed mammalian milk and by-products in mammalian milk-processing, without losing the physiological activities of lactoferrin, which comprises adjusting pH of said moisturized or liquefied lactoferrin contained in said matter within an acidic range between 2.0 and 6.0 both inclusive by adding acid or aqueous solution of acid when the pH of said moisturized or liquefied lactoferrin is out of said pH range, and heating said matter in the range from 60.degree. C. to 130.degree. C. for a span of time which may assure 60% or more of undenaturization rate of lactoferrin.

Abstract: Bioactive agents, having growth-promoting activity upon Bifidobacteria, consisting of one or more lactoferrin-compounds selected from the group consisting of bovine lactoferrin, bovine apolactoferrin and bovine Fe-lactoferrin, which can be used as it is as agents to promote proliferation of Bifidobacteria both in vivo and in vitro, and as additives to prepare compositions, materials and products to afford growth-promoting activity thereto, and to improve survivability of Bifidobacteria, if contained therein.

Abstract: Lactoferricin (Trade Mark), also called LFCIN (Trade Mark), a potent antimicrobial peptide, is produced by contacting an enzymatic hydrolysate of bovine lactoferrin or any mixture of peptides containing lactoferricin preferably with a butyl moiety-containing hydrophobic interaction chromatography medium, or alternatively with a carboxymethyl moiety-containing cation-exchange chromatography medium, rinsing the medium to remove unbound peptides, desorbing the lactoferricin solution at constant pH, and desalting the desorbed solution. The utility of the process is illustrated, for example, as follows. An enzymatic hydrolysate of bovine lactoferrin (600 g) was contacted with 3000 ml of BUTYL-TOYOPEARL 650M, the medium was rinsed with water, and then with McIlvaine (citric acid-sodium phosphate) buffer at pH 7.0., and lactoferricin was desorbed with McIlvaine buffer at pH 5.0.

Abstract: A milk-protein hydrolyzate consisting of a mixture of peptides and free amino acids having proliferation activating property on human cutaneous cells but not having antigenicity of the milk-protein may be obtained by enzymatic hydrolysis of milk protein. The peptides of the hydrolyzate have molecular weights less than 1000 daltons, and the hydrolyzate has a free aromatic amino acid/total aromatic amino acid ratio of at least 90%. Fractionation of the milk protein hydrolyzate yields a fraction consisting of a mixture of peptides. The fraction has a proliferation activating property on human cutaneous cells but does not have the antigenicity of the milk protein. The fraction contains aromatic amino acids in an amount of less than 5% by weight of total amino acids. Both the hydrolyzate and the fraction can be formulated into cosmetic compositions for application to the hair and skin.

Abstract: An antimicrobial peptide containing at least the following amino acid sequence (a), (b), (c) or (d), an antimicrobial agent containing the said antimicrobial peptide as active components at a concentration of at least 5 ppm (weight), an antimicrobial composition the said antimicrobial peptide and a method for processing products which uses the antimicrobial agent containing the said antimicrobial peptide.

Abstract: A crystalline lactulose trihydrate having molecular formula of C.sub.12 H.sub.22 O.sub.11.3H.sub.2 O and a method for its manufacture, which comprises crystallization from a lactulose syrup at a temperature of 2.degree.-20.degree. C., the starting lactulose syrup having a lactulose content of 70-90% of a total solid matters by weight, and having been concentrated to the total solid matters of 65-75% by weight and a ratio of lactose to water of less than 10% by weight.

Abstract: Bioactive agents consisting of one or more lactoferrin-compounds selected from the group consisting of Zn-, Cu- and Mn-lactoferrin and having selective biological activities, i.e. growth-inhibitory activity against certain harmful microorganisms on one hand and growth-promoting activity upon certain useful microorganisms on the other hand; as well as compositions, products or materials therefor comprising the bioactive agents consisting of one or more lactoferrin-compounds selected from the group; and a method for treating materials, which are edible for human beings or animals or which are applicable to a portion of body of human beings or animals, with the bioactive agents consisting of one or more lactoferrin-compounds selected from the group consisting of Zn-, Cu- and Mn-lactoferrin or the compositions comprising the bioactive agent consisting of one or more lactoferrin-compounds selected from the group consisting of Zn-, Cu- and Mn-lactoferrin as an effective component.

Abstract: This invention relates to a reverse osmosis treatment process, particularly a stable reverse osmosis treatment process which prevents a lowering in throughput capacity by carrying out treatment while keeping the permeate flux of a reverse osmosis membrane constant through the time of use of a module. The process of this invention can keep the throughput capacity constant, can prevent adhesion and pollution onto membrane surfaces and fouling thereon, and moreover can maintain stable operation in a continuous run.

Abstract: An agent for tyrosinase inhibition which contains the effective quantity of enzymatic hydrolyzates of milk proteins, having a decomposition rate from 6% to 55% as expressed by the percentage of formol nitrogen to total nitrogen.

Abstract: Lactoferrin hydrolyzates, having a decomposition rate between 6%-20% as measured by formol titration, for use as an antibacterial agent and which have remarkly more potent activity than unhydrolyzed lactoferrin; and lactoferrin hydrolyzates, having a decomposition rate between 4-50% as measured by formol titration, for use as a tyrosinase inhibition agent, are obtainable by conventional methods for hydrolysis with acids or enzymes, and are stable to heating.

Abstract: A composition of feedstuff containing at least 0.5 wt. %, in solid content, of residual liquid of incubated culture broth of Bifido-bacteria and/or lactic acid bacteria which residual liquid is obtainable as the waste by-product of mass production of living or viable cell mass of the microorganisms by substantially excluding microbial cell mass from the incubated culture broth thereof.

Abstract: Dietary fibers having average molecular weight of 2,000-15,000, which are prepared by partially hydrolyzing polysaccharides of Konnyaku powder with a cellulase derived from microorganisms belonging to the genus Aspergillus, which are readily soluble in water, which do not prevent adsorption of useful minerals such as calcium in the digestive tract, and which do not give rise to diarrhea when an excessive amount thereof is ingested.

Abstract: A grandular product is prepared by fluidizing core material such as saccharide in a granulating chamber, spraying melted binding material such as fat to the fluidized core material and concurrently feeding dried viable microorganism cells such as bifidobacterium towards the fluidized core material to form a granular product having stratified structure by adhering microorganism cells on the periphery of the core material. Since the product is prevented from permeation of atmospheric oxygen and environmental moisture, the cell survival rate of the product is higher than that of the conventional product during prolonged storage periods.

Abstract: A process for producing bovine lactoferrin in high purity form raw milk-materials containing skim milk or whey originated form cow's milk which comprises: adsoption step wherein said raw materials are contacted, at a temperature between 0.degree.-60.degree. C., with weakly acidic cation-exchanger which includes carboxymethyl groups as ion exchanging groups and has haemoglobin adsorption property more than 3.5 g/100 ml; rinsing step wherein said exchanger is washed with water to remove substances other than those adsorbed to said exchanger; and desorption step wherein the substances adsorbed to said exchanger are desorbed therefrom with a solution of one or more of salts to thereby yield highly purified bovine lactoferrin without deterirating raw milk materials. Said desorption step can be carried out in a single stage to produce lactoferrin more than 80% purity to total proteins recovered, and in two stage to attain more than 98% purity.

Abstract: This invention relates to instant creaming powder enriched with flavor and/or color which contains at least 0.2% by weight of fine particles prepared by locking a small amount of flavor in a mixture of a saccharide dehydrate and a saccharide, or coloring the mixture, or subjecting the mixture to both of these treatments, to obtain suitable solubility, creaminess and whitening effect equal to those of the conventional instant creaming powder in spite of incorporating thereinto the fine particles for enrichment with flavor, and has a novel appearance in which fine particles having crystalline brilliance are uniformly dispersed in the instant creaming powder.

Abstract: Method for manufacturing a lactulose powder with a low hygroscopicity by mixing ethyl alcohol to a highly hygroscopic lactulose-containing powder followed by allowing the resulting mixture to stand or stirring it for at least 1 hour, separating the lactulose-containing material insoluble in ethyl alcohol from the mixture and removing ethyl alcohol from the material separated.

Abstract: A process for preparing a free-flowing lactulose-containing powder from a solution containing lactose, by adding calcium hydroxide to the solution to adjust the pH of said solution to from 9.4 to 11.2; heating the resulting solution so that the pH is reduced to from 7.5 to 9.0; the homogenizing, concentrating and drying.