Abstract: The present disclosure provides a cartridge for purifying biological samples. The cartridge comprises a first chamber configured to receive and hold biological samples, and at least one second chamber configured to receive and hold a reagent solution. A first fluid flow control valve is fluidly connected to outlet ports of the first chamber and the at least one second chamber. Also, the first fluid flow control valve is fluidly connected to a matrix chamber and is configured to selectively route biological samples and reagent solution into the matrix chamber for purifying the biological samples. A binding matrix is configured in the matrix chamber for capturing the nucleic acids from the biological samples and the reagent solution. Further, a elute collection chamber is fluidly connected to the matrix chamber, wherein the elute collection chamber is configured to receive and hold purified biological sample from the matrix chamber.

Abstract: The present disclosure provides a portable device for purifying biological sample. The device comprises a housing and a cartridge holder accommodated in the housing for holding at least one cartridge configured to purify the biological sample. At least one pump is positioned in the housing which is fluidly connectable to the cartridge to circulate the sample and one or more fluids through the cartridge. Further, at least one heating element is configured to heat a matrix chamber in the cartridge. At least one actuator is disposed in the housing to actuate one or more valves to selectively route the biological sample and the one or more fluids in the cartridge. A control unit is interfaced with the actuator, the heating element and the pump and is configured to regulate operation of the actuator, the pump and the heating element during purification of the biological sample.

Abstract: The present disclosure discloses a cartridge for purifying a sample. The cartridge comprising a first chamber having a plurality of compartments for storing a sample and at least one reagent and mix the a sample with the at least one reagent. A second chamber in fluid communication with the first chamber is configured with a matrix member for matrixing at least one analyte. A third chamber in fluid communication with the second chamber is configured with a waste collection chamber for storing waste fluids matrixed in the second chamber. A fourth chamber in fluid communication with the third chamber, includes at least one tube configured to receive and store the at least one analyte from the second chamber, through the third chamber. The cartridge enables purification of multiple samples in a cycle.

Abstract: The present disclosure provides a portable device for purifying biological sample. The device comprises a housing and a cartridge holder accommodated in the housing for holding at least one cartridge configured to purify the biological sample. At least one pump is positioned in the housing which is fluidly connectable to the cartridge to circulate the sample and one or more fluids through the cartridge. Further, at least one heating element is configured to heat a matrix chamber in the cartridge. At least one actuator is disposed in the housing to actuate one or more valves to selectively route the biological sample and the one or more fluids in the cartridge. A control unit is interfaced with the actuator, the heating element and the pump and is configured to regulate operation of the actuator, the pump and the heating element during purification of the biological sample.

Abstract: The present disclosure provides a cartridge for purifying biological samples. The cartridge comprises a first chamber configured to receive and hold biological samples, and at least one second chamber configured to receive and hold a reagent solution. A first fluid flow control valve is fluidly connected to outlet ports of the first chamber and the at least one second chamber. Also, the first fluid flow control valve is fluidly connected to a matrix chamber and is configured to selectively route biological samples and reagent solution into the matrix chamber for purifying the biological samples. A binding matrix is configured in the matrix chamber for capturing the nucleic acids from the biological samples and the reagent solution. Further, a elute collection chamber is fluidly connected to the matrix chamber, wherein the elute collection chamber is configured to receive and hold purified biological sample from the matrix chamber.

Abstract: The present disclosure gives description of a method used for the detection and quantification of dengue viral infection caused by dengue virus using nucleic acids isolated from blood, plasma or serum samples by employing Oligonucleotide probes. The method employed here for detection is by Real time PCR. The instant disclosure also provides for primers, probes, PCR Reaction mixture and kit thereof.