Abstract: Disclosed herein is a single-use extraction apparatus which is disposed of after use. The extraction apparatus includes a tube plate having a tube therein, and a column plate. The column plate is detachably coupled to the tube plate, and includes a column which corresponds to the tube of the tube plate. A filter is provided in the column and made of a material which adsorbs or desorbs biochemical materials included in biological samples. A hollow suction port is provided at a predetermined position of the column plate to connect to a vacuum pump. The apparatus is capable of extracting biochemical materials from biological samples by using vacuum pressure without centrifugal separation, thus realizing a simple, convenient and rapid extracting operation, and has a small size so that it rapidly reaches desired vacuum pressure and uniform vacuum pressure can act on the column.

Abstract: The present invention relates to a kit for screening and establishing optimal amplification condition for individual PCR using a dried-formulated PCR reagent which is composed of different combinations of various components affecting PCR result, to provide a method for screening and establishment of optimal amplification in PCR constructed with a dried-formulated PCR reagent. According to the present invention, researchers can perform PCR under the optimal amplification conditions appropriate for individual PCR even with a PCR-related product constructed from a dried-formulated PCR reagent, suggesting that a unique target gene can be efficiently amplified by PCR constructed with a dried-formulated PCR reagent under the more appropriate conditions.