Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

Abstract: Drug supply units are disclosed which comprise substrates having a plurality of holes formed therein.

Abstract: Methods of transferring material from a first device having an array of microwells to a second device is provided. In some examples, the first device and the second device are moved together toward a stopper plate and impinge on the stopper plate. In other examples, the first device and the second device are kept stationary and an impinging device is impacted on a mounting structure enclosing the first and second devices, causing material transfer from the microwells of the first device to the second device. Apparatus for carrying out the transfer of material is also disclosed.

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

Abstract: Drug supply units are disclosed which comprise substrates having a plurality of holes formed therein.

Abstract: A method of forming a polymer matrix array includes treating a surface within a well of a well array with a surface compound including a surface reactive functional group and a radical-forming distal group; applying an aqueous solution including polymer precursors to the well of the well array; and activating the radical-forming distal group of the surface coupling compound with an initiator and atom transfer radical polymerization (ATRP) catalyst to initiate radical polymerization of the polymer precursors within the well of the well array to form the polymer matrix array.

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

Abstract: A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

Abstract: A method of fluorescent in-situ hybridization imaging includes exposing a sample to a first plurality of first readout probes and a second plurality of second readout probes, obtaining a first image of the sample with first readout probes and the second readout probes bound to a first analyte and a second analyte, respectively, so as to emit light at a first wavelength range, treating the sample so as to modify the second readout probes, and obtaining a second image of the sample with the first readout probes bound to the first analyte so as to emit light at the first wavelength range and the second analyte substantially not emitting light at the first wavelength range.

Abstract: A computer program product includes instructions to cause one or more computers to obtain first and second images in the same color channel in the same hybridization step from a fluorescent in-situ hybridization imaging system, and to register the first image and the second image. One or more pixels having a first intensity exceeding a first threshold in the first image and having a second intensity exceeding a second threshold in the second image are identified and classified as activated in a first readout call, and one or more pixels having the first intensity exceeding the first threshold in the first image and the second intensity below the second threshold in the second image are identified and classified as activated in a second readout call.

Abstract: A readout probe for use in fluorescent in-situ hybridization imaging has a targeting portion to bind to a first hybridization sequence in an encoding probe that targets an analyte in a sample, a first fluorophore to emit light at a wavelength range, and a first cleaving region between the first fluorophore and the targeting portion.

Abstract: Methods of transferring material from a first device having an array of microwells to a second device is provided. In some examples, the first device and the second device are moved together toward a stopper plate and impinge on the stopper plate. In other examples, the first device and the second device are kept stationary and an impinging device is impacted on a mounting structure enclosing the first and second devices, causing material transfer from the microwells of the first device to the second device. Apparatus for carrying out the transfer of material is also disclosed.

Abstract: A method of transferring material using a picking pin having a distal tip includes: forming a first coating of organic molecules on at least a portion of the distal tip of the picking pin; dipping at least a portion of the coated distal tip in a first source such that an amount of material is picked up from the first source and attached to the first coating; dipping the coated distal tip attached with the amount of material into a first target so as to release some of the amount of the material to the first target; and removing the first coating from the distal tip. The procedure can be repeated for transferring additional material from the same or different source to the same or different targets.

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

Abstract: A system for loading a sample into microwells of a microfabricated chip. The system can include a vacuum loading module, and can further include a sealing module. The loading module includes enclosed chamber formed a bottom part and a top part. The chamber is provided with at least one vacuum port. The chamber can also be provided with an injection port for injecting a liquid sample into the chamber to thereby load the sample into the microwells of the microchip. The sealing module includes a wheel carrying a sealing film and a mounting platform to position a microfabricated chip, where the rotation of the wheel on the microfabricated chip transfers the sealing film on the top surface of the microfabricated chip. Methods of loading a sample onto the microfabricated chip and sealing the loaded chip are also provided.

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

Abstract: A method of modifying a microfabricated chip made of a hydrophobic material is provided. The microfabricated chip has a top surface including a plurality of microwells each having a bottom and a side wall, and interstitial space between the microwells. First, the ed chip is surface treated to render the surface of the bottom and side wall of the microwells and the interstitial space hydrophilic. Then, the surface of the interstitial space is treated to render it hydrophobic. A microfabricated chip having hydrophilic microwells and hydrophobic interstitial space is also provided.

Abstract: A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.