Abstract: The present application relates to a freeze-dried polymer composition containing chitosan and at least one lyoprotectant, a process for preparing a freeze-dried composition containing chitosan and at least one lyoprotectant and the use of a reconstituted freeze-dried chitosan composition to prepare implants for tissue repair.

Abstract: The present application relates to a freeze-dried polymer composition containing chitosan and at least one lyoprotectant, a process for preparing a freeze-dried composition containing chitosan and at least one lyoprotectant and the use of a reconstituted freeze-dried chitosan composition to prepare implants for tissue repair.

Abstract: The present disclosure relates to a nucleic acid delivery composition comprising a coated chitosan-based polyplex, wherein the coated chitosan-based polyplex comprises: a chitosan; an isolated nucleic acid; and an additional polyelectrolyte. The coated chitosan-based polyplex has an initial or a final molar ratio of amine groups of chitosan (N) to phosphate groups of the nucleic acid (P) to carboxyl groups of the additional polyelectrolyte (C) (N:P:C), wherein the N has a value between about 1.0 and about 10.5, the P has a value between about 1.0 and about 2.0 and the C has a value between about 1.0 and 10.5.

Abstract: The present description relates to a polymer composition for use in repairing tissue of a patient comprising at least one blood component, as polymer, such a chitosan, and at least one inorganic salt, such as NaCl, method of the composition and method of preparing the composition.

Abstract: The present application relates to a freeze-dried polymer composition containing chitosan and at least one lyoprotectant, a process for preparing a freeze-dried composition containing chitosan and at least one lyoprotectant and the use of a reconstituted freeze-dried chitosan composition to prepare implants for tissue repair.

Abstract: The present invention relates to a polyelectrolyte complex composition comprising a polymer, a nucleic acid molecule, a lyoprotectant, and a buffer. The polyelectrolyte complex composition preserving the biological activities of the polyelectrolyte complex following freeze-drying and rehydration.

Abstract: The present description relates to a polymer composition for use in repairing tissue of a patient comprising at least one blood component, a polymer, such as chitosan, and at least one inorganic salt, such as NaCl, method of using the composition and method of preparing the composition.

Abstract: There is disclosed aqueous solutions of polyelectrolytes that can be neutralized by increasing temperature, under suitable polyelectrolyte charge state conditions, in order to obtain a homogeneous gel. This can be achieved by adding an appropriate weak electrolyte to the polyelectrolyte so that an increase of temperature will lead to a spatially homogeneous neutralization of the polyelectrolyte via proton transfer between the polyelectrolyte and the weak electrolyte. The ability of such a system to be thermally sensitive and to gel upon heating relies on the temperature dependence of the ionization equilibrium for the two components. This thermally induced neutralization reduces electrostatic repulsion between polyelectrolyte molecules allowing the manifestation of attractive polyelectrolyte-polyelectrolyte hydrophobic interactions and hydrogen bonding that result in gel formation. These new kinds of thermo sensitive gels can be used for biomedical applications.

Abstract: There is disclosed aqueous solutions of polyelectrolytes that can be neutralized by increasing temperature, under suitable polyelectrolyte charge state conditions, in order to obtain a homogeneous gel. This can be achieved by adding an appropriate weak electrolyte to the polyelectrolyte so that an increase of temperature will lead to a spatially homogeneous neutralization of the polyelectrolyte via proton transfer between the polyelectrolyte and the weak electrolyte. The ability of such a system to be thermally sensitive and to gel upon heating relies on the temperature dependence of the ionization equilibrium for the two components. This thermally induced neutralization reduces electrostatic repulsion between polyelectrolyte molecules allowing the manifestation of attractive polyelectrolyte-polyelectrolyte hydrophobic interactions and hydrogen bonding that result in gel formation. These new kinds of thermo sensitive gels can be used for biomedical applications.

Abstract: There is disclosed a composition and a method for the efficient non-viral delivery of nucleic acids to cells using chitosan. In order to achieve high efficiency of transfection, the composition contains a nucleic acid and a chitosan that has the following physico-chemical properties: a combination of a number-average molecular weight between 8 kDa and 185 kDa and a degree of deacetylation between 72% and 92%. The chitosan molecule can also present additional physiochemical properties such as a block distribution of acetyl groups obtained by a heterogeneous treatment of chitin, and/or a polydispersity index between 1.4 and 7.0. By correctly controlling these parameters, efficient delivery systems may be produced that are effective when optimized for different conditions such as the pH of transfection media and amine-to-phosphate ratio.

Abstract: A universal material testing device which includes a frame; an actuator mounted on the frame for controlling a displacement of a sample to be tested; a load cell movably mounted on the frame and adapted to abut against the sample for detecting a force applied thereon by the actuator, the load cell producing a signal corresponding to the force detected; a signal conditioning unit for reducing input noise and for processing of the signal and executing specific tests by coordination of displacement control and load signals received for processing from the load cell; and a detachable chamber with humidifying media for humidification of the sample environment confined within the chamber and separated frm the sample so as to avoid potential damaging effects of humidity on the sample or on the device. The device is useful for determining certain material properties of a sample.