Abstract: The instant disclosure is generally related to compositions and methods for obtaining and constructing Bacillus licheniformis host cells (e.g., protein production host cells, cell factories) having increased protein production capabilities. Certain embodiments of the disclosure are directed to efficient genetic modifications of B. licheniformis cells and the subsequent selection of such B. licheniformis cells having increased protein production capabilities. Certain other embodiments of the disclosure are generally related to methods and compositions for producing/obtaining auxotrophic B. licheniformis cells, wherein certain other embodiments of the disclosure are directed to methods and compositions for restoring prototrophy in auxotrophic B. licheniformis cells, and expressing genes of interest (GOIs) in such restored prototrophy B. licheniformis cells.

Abstract: A pedestrian protective barrier includes a base barrier and a removable lid that engages with and covers the base barrier. The protective barrier may be made from a resin, plastic, or other suitable material that satisfies the properties and features herein. By using plastic or resin, for example, the protective barrier is sufficiently light to enable easy storage and transportation. The base barrier includes an interior cavity to receive and accommodate a weighted material, such as water, dirt, sand, weights, etc., once the protective barriers are arranged in a desired place, such as in the street protecting a restaurant's dining area, each base barrier's cavity may be filled with a desired weighted material.

Abstract: A method for producing a pre-insulated pipe, comprising inserting a length of jacket pipe (10) into a guide channel (110) having a front end (111) and a rear end (112); fixing said length of jacket pipe (10) in said guide channel (110); providing a first end (21) of a length of insulated inner piping (20), said length of insulated inner piping comprising a length of inner pipe surrounded by at least one layer of compressible insulation material (26); inserting, at said front end of said guide channel, into said length of jacket pipe, said first end of said length of insulated inner piping (20); applying an overpressure at least in an interior of said length of jacket pipe (10), around the inserted first end of the length of insulated inner piping (20); and moving said first end of said length of insulated inner piping (20) to said rear end of said guide channel (110); wherein said overpressure is such that said at least one layer of insulation material (26) is radially compressed; removing said overpressure t

Abstract: A method for producing a pre-insulated pipe, comprising inserting a length of jacket pipe (10) into a guide channel (110) having a front end (111) and a rear end (112); fixing said length of jacket pipe (10) in said guide channel (110); providing a first end (21) of a length of insulated inner piping (20), said length of insulated inner piping comprising a length of inner pipe surrounded by at least one layer of compressible insulation material (26); inserting, at said front end of said guide channel, into said length of jacket pipe, said first end of said length of insulated inner piping (20); applying an overpressure at least in an interior of said length of jacket pipe (10), around the inserted first end of the length of insulated inner piping (20); and moving said first end of said length of insulated inner piping (20) to said rear end of said guide channel (110); wherein said overpressure is such that said at least one layer of insulation material (26) is radially compressed; removing said overpressure t

Abstract: A method for producing a pre-insulated pipe, comprising inserting a length of jacket pipe (10) into a guide channel (110) having a front end (111) and a rear end (112); fixing said length of jacket pipe (10) in said guide channel (110); providing a first end (21) of a length of insulated inner piping (20), said length of insulated inner piping comprising a length of inner pipe surrounded by at least one layer of compressible insulation material (26); inserting, at said front end of said guide channel, into said length of jacket pipe, said first end of said length of insulated inner piping (20); applying an overpressure at least in an interior of said length of jacket pipe (10), around the inserted first end of the length of insulated inner piping (20); and moving said first end of said length of insulated inner piping (20) to said rear end of said guide channel (110); wherein said overpressure is such that said at least one layer of insulation material (26) is radially compressed; removing said overpressure t

Abstract: A method for producing a pre-insulated pipe, comprising inserting a length of jacket pipe (10) into a guide channel (110) having a front end (111) and a rear end (112); fixing said length of jacket pipe (10) in said guide channel (110); providing a first end (21) of a length of insulated inner piping (20), said length of insulated inner piping comprising a length of inner pipe surrounded by at least one layer of compressible insulation material (26); inserting, at said front end of said guide channel, into said length of jacket pipe, said first end of said length of insulated inner piping (20); applying an overpressure at least in an interior of said length of jacket pipe (10), around the inserted first end of the length of insulated inner piping (20); and moving said first end of said length of insulated inner piping (20) to said rear end of said guide channel (110); wherein said overpressure is such that said at least one layer of insulation material (26) is radially compressed; removing said overpressure t