Patents by Inventor Marcelo B. Soares

Marcelo B. Soares has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for construction of normalized cDNA libraries
    Patent number: 5830662
    Abstract: This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.
    Type: Grant
    Filed: June 21, 1996
    Date of Patent: November 3, 1998
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Marcelo B. Soares, Argiris Efstratiadis
  • Normalized cDNA libraries
    Patent number: 5637685
    Abstract: This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.
    Type: Grant
    Filed: June 6, 1995
    Date of Patent: June 10, 1997
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Marcelo B. Soares, Argiris Efstratiadis
  • Method for construction of normalized cDNA libraries
    Patent number: 5482845
    Abstract: This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.
    Type: Grant
    Filed: September 24, 1993
    Date of Patent: January 9, 1996
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Marcelo B. Soares, Argiris Efstratiadis