Abstract: This disclosure provides a method of identifying or separating activated regulatory T cells that have a demethylated Foxp3 TSDR region from a mixture of activated T cells. The mixture is depleted of cells bearing the CD154 molecule (CD40 ligand). The desired cells can be recovered using a Treg marker such as CD25, GITR, CTLA4, CD137, latent TGF-beta (LAP), GARP (LRRC32) or CD121a/b. The Treg cells having a demethylated Foxp3 TSDR region can be used, for example for preparation of pharmaceutical compositions for use in treatment.

Abstract: The present invention relates to a method of identifying and separating non-regulatory T-cells (conventional T-cells) from a mixture comprising regulatory T-cells by using of the CD154 molecule (CD40 ligand) through depletion of CD154+ T-cells from the mixture or in combination with additional positive selection of Treg using markers that are specific for regulatory T-cells, such as for example, CD25, GITR, CTLA4 or markers which are specific for activated regulatory T-cells, such as, for example, CD137, “latent TGF-beta (LAP)”, GARP (LRRC32), CD121a/b, thereby generating a cell composition of activated Treg cells. The invention relates also to a kit comprising an antibody for detecting CD154 and at least one additional antibody for detecting markers for activated or non-activated regulatory T-cells. The antibodies can be coupled to a fluorescent dye or magnetic microparticles.

Abstract: The invention relates to the use of the 4-1BB receptor for identifying and/or separating specific regulatory Th cells after activation with an antigen, polyclonal regulatory Th cells after polyclonal activation and to a method for the identification and/or separation of regulatory Th cells, the regulatory T cells being detected on the basis of the expression of the 4-1BB receptor by an antibody, an antigen or ligands which are coupled to a fluorescent substance, haptenes or magnetic microparticles. The invention also relates to a kit comprising an antibody, an antigen or ligands for detecting the 4-1BB receptor for the identification and/or separation of a regulatory Th cell, the antibody being coupled to a fluorescent substance, haptenes or magnetic microparticles.

Abstract: The present invention relates to a method of identifying and separating non-regulatory T-cells (conventional T-cells) from a mixture comprising regulatory T-cells by using of the CD154 molecule (CD40 ligand) through depletion of CD154+ T-cells from the mixture or in combination with additional positive selection of Treg using markers that are specific for regulatory T-cells, such as for example, CD25, GITR, CTLA4 or markers which are specific for activated regulatory T-cells, such as, for example, CD137, “latent TGF-beta (LAP)”, GARP (LRRC32), CD121a/b, thereby generating a cell composition of activated Treg cells. The invention relates also to a kit comprising an antibody for detecting CD154 and at least one additional antibody for detecting markers for activated or non-activated regulatory T-cells. The antibodies can be coupled to a fluorescent dye or magnetic microparticles.

Abstract: The invention relates to the use of the 4-1BB receptor for identifying and/or separating specific regulatory Th cells after activation with an antigen, polyclonal regulatory Th cells after polyclonal activation and to a method for the identification and/or separation of regulatory Th cells, the regulatory T cells being detected on the basis of the expression of the 4-1BB receptor by an antibody, an antigen or ligands which are coupled to a fluorescent substance, haptenes or magnetic microparticles. The invention also relates to a kit comprising an antibody, an antigen or ligands for detecting the 4-1BB receptor for the identification and/or separation of a regulatory Th cell, the antibody being coupled to a fluorescent substance, haptenes or magnetic microparticles.

Abstract: What is suggested herein is the use of CD154 for the detection and/or isolation of antigen-specific T lymphocytes and a method for the detection and/or isolation of antigen-specific T lymphocytes, wherein the suspension is contacted with a CD40/CD154 system inhibitor, intra- or extracellular determination of CD154 is effected, and the cells having CD154 are detected and/or isolated.

Abstract: What is suggested herein is the use of CD154 for the detection and/or isolation of antigen-specific T lymphocytes and a method for the detection and/or isolation of antigen-specific T lymphocytes, wherein the suspension is contacted with a CD40/CD154 system inhibitor, intra- or extracellular determination of CD154 is effected, and the cells having CD154 are detected and/or isolated.