Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.

Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.

Abstract: A floor-cleaning machine which can be driven over the floor, includes a driven brush for sweeping the floor to be cleaned. The brush picks up dirt and debris from the floor and carries it, assisted by forced air flow, in an overhead motion and through a transfer duct to a pair of dirt containers in the form of buckets located side-by-side at the rear of the machine. A manual linkage at the rear of the machine moves a pivoted carrier frame which holds the dirt containers, between a raised use position in which the upper rims of the dirt containers are sealed to the machine for collection of debris, and a lowered access position for removal of the containers. Each container is independently accessible for emptying, and includes a bail-type handle pivotally attached such that a bucket, the opening of which faces forwardly and upwardly in the use position, extends generally horizontal when removed, to avoid spillage.

Abstract: The present invention relates to a method of identifying a gene or gene product associated with transcription dependent memory formation in an animal comprising the steps of: (a) administering to said animal sufficient small interfering RNA (siRNA) specific for the gene to inhibit gene function; (b) training said animal under conditions sufficient to induce transcription dependent memory formation in a normal untreated animal; and (c) determining the level of transcription dependent memory formation induced by the training of the treated animal. The present invention provides methods of using small interfering PNAs (siRNA) in hippocampus to identify genes and gene product whose inhibition affects contextual and temporal long-term (LTM) memory, but not short-term memory (STM).

Abstract: A floor-cleaning machine which can be driven over the floor, includes a driven brush for sweeping the floor to be cleaned. The brush picks up dirt and debris from the floor and carries it, assisted by forced air flow, in an overhead motion and through a transfer duct to a pair of dirt containers in the form of buckets located side-by-side at the rear of the machine. A manual linkage at the rear of the machine moves a pivoted carrier frame which holds the dirt containers, between a raised use position in which the upper rims of the dirt containers are sealed to the machine for collection of debris, and a lowered access position for removal of the containers. Each container is independently accessible for emptying, and includes a bail-type handle pivotally attached such that a bucket, the opening of which faces forwardly and upwardly in the use position, extends generally horizontal when removed, to avoid spillage.

Abstract: The present invention provides methods for screening a pharmaceutical agent for its ability to modulate long term memory formation, performance of a hippocampal-dependent cognitive task or Gpr12 function. The present invention also provides methods for modulating long term memory formation or performance of a hippocampal-dependent cognitive task by modulating Gpr12-dependent protein expression. The present invention further provides methods for treating a defect in long term memory formation by inhibiting Gpr12 function and methods for treating a defect in performance of a hippocampal-dependent cognitive task by inhibiting Gpr12 function.

Abstract: A floor-cleaning machine which can be driven over the floor, includes a driven brush for sweeping the floor to be cleaned. The brush picks up dirt and debris from the floor and carries it, assisted by forced air flow, in an overhead motion and through a transfer duct to a pair of dirt containers in the form of buckets located side-by-side at the rear of the machine. A manual linkage at the rear of the machine moves a pivoted carrier frame which holds the dirt containers, between a raised use position in which the upper rims of the dirt containers are sealed to the machine for collection of debris, and a lowered access position for removal of the containers. Each container is independently accessible for emptying, and includes a bail-type handle pivotally attached such that a bucket, the opening of which faces forwardly and upwardly in the use position, extends generally horizontal when removed, to avoid spillage.

Abstract: Known wired/wireless terminals for browsing an internet/intranet and comprising a browser function running via a processor and a memory for said browsing and comprising a man-machine-interface for controlling said browser function sometimes completely block (browser-hang-up situation), which can only be changed by switching said terminal off and on. By providing said terminal with a generator for generating an overrule signal for overruling said browser function, in case a browser-hang-up situation appears, there is no longer a need to switch said terminal off and on. Possibly, said overruling is done in combination with amending said browsing in response to a response signal originating from a network-unit. Preferably, said overruling and/or said amending comprises a generation of a previous address signal, and/or a reset of said browser function, and/or a finish of said browser function.

Abstract: Soundproofed conduit to discharge fumes, advantageously associated with assemblies to discharge fumes for melting systems in steel production plants, the conduit being located between the ventilation system (14) and an outlet chimney (13), at least the first segment (19a) of the discharge conduit defining a section with a value (A) through which the fumes transit, the conduit (12) defining a path, from the relative ventilation system (14) to the discharge chimney (13), comprising at least two counter-opposed and controlled changes in direction (15a, 15b) defining at least respective consecutive segments (19a, 19b, 19c) arranged one at an angle to another, the changes in direction being functionally configured so as to cause phase and counter-phase effects of the sound waves generated by the fluid in transit, the phase and counter-phase effects having the purpose of at least deadening partly the sound waves caused by the passage of the fluid.