Abstract: The present invention is related to a system for the heterologous expression of a monoclonal Antibody (mAb) or a fragment or derivative thereof, said system comprising at least one ciliate host cell, and incorporated, into said ciliate host cell, at least one heterologous nucleic acid molecule encoding for said monoclonal Antibody, or a fragment or derivative thereof.

Abstract: The present invention relates to a medical treatment arrangement having at least three device parts, with the first device part being a device part which is not made for the treatment of a patient, with the second device part being made in conjunction with the third device part such that a treatment of a patient can be made with them, with the second device part being made movable relative to the first device part and relative to the third device part, and with provision furthermore being made that the first device part and the second device part and/or the second device part and the third device part being made such that energy and/or data can be unidirectionally or bidirectionally exchanged between them.

Abstract: The present invention is related to a system for the heterologous expression of a monoclonal Antibody (mAb) or a fragment or derivative thereof, said system comprising at least one ciliate host cell, and incorporated, into said ciliate host cell, at least one heterologous nucleic acid molecule encoding for said monoclonal Antibody, or a fragment or derivative thereof.

Abstract: The present invention relates to a medical treatment arrangement having at least three device parts, with the first device part being a device part which is not made for the treatment of a patient, with the second device part being made in conjunction with the third device part such that a treatment of a patient can be made with them, with the second device part being made movable relative to the first device part and relative to the third device part, and with provision furthermore being made that the first device part and the second device part and/or the second device part and the third device part being made such that energy and/or data can be unidirectionally or bidirectionally exchanged between them.

Abstract: The present invention relates to a system for the heterologous expression of a viral protein or a fragment thereof, said system comprising a) a ciliate host cell, b) at least one cDNA encoding for a viral protein, or a fragment thereof, and c) a promoter operably linked to said cDNA.

Abstract: A method for identifying a protease secretion deficient strain of a microorganism involves producing a mutant of the microorganism, followed by adding the mutant to gel-filled wells of a microtitration plate, incubating the mutant in the gel-filled wells under conditions call sing the mutant to secrete proteins, separating the mutant from the gel, and measuring activity of a secretion protease of the microorganism in the gel, wherein either (i) the gel is a substrate for the secretion protease or (ii) the gel contains a substrate for the secretion protease.

Abstract: A method for producing a ciliate cell with reduced or essentially no dihydrofolate reductase (DHFS) activity or reduced or essentially no thymidylate synthase (TS) activity or both reduced or essentially no dihydrofolate reductase and thymidylate synthase (DHFR-TS) activity is claimed, comprising the steps of a) transforming ciliate cells by inserting a construct containing an allele altering the gene encoding the endogenous DHFR-TS into at least one of the endogenous DHFR-TS genes of the ciliate macronucleus (MAC), b) inducing an allelic assortment process in the transformed ciliate cells to generate cells having the construct inserted in most or all functional DHFR-TS genes of the MAC, and c) identifying the cells generated in step b) by cultivation with or without thymidine.

Abstract: The present invention is based on the finding that a capacitance can be measured precisely and efficiently when, in a delta-sigma modulator having an operational amplifier, a first capacitor connectable to an input of the operational amplifier, and a second capacitor in a feedback branch of the operational amplifier, a reference signal source is connectable to the first capacitor, wherein the first or second capacitor may represent a capacitance to be measured. Due to the fact that, in contrast to what is conventional, no input quantity is measured and digitalized at the input of the delta-sigma modulator, but instead a defined reference signal source is coupled at the input and a device of the delta-sigma modulator itself represents the measuring quantity, an extremely compact circuit is provided allowing capacitances to be measured quickly and reliably, the measuring result being additionally made available in a digital form.

Abstract: A method for identifying a protease secretion deficient strain of a microorganism involves producing a mutant of the microorganism, followed by adding the mutant to gel-filled wells of a microtitration plate, incubating the mutant in the gel-filled wells under conditions call sing the mutant to secrete proteins, separating the mutant from the gel, and measuring activity of a secretion protease of the microorganism in the gel, wherein either (i) the gel is a substrate for the secretion protease or (ii) the gel contains a substrate for the secretion protease.

Abstract: The present invention relates to a system for the heterologous expression of a viral protein or a fragment thereof, said system comprising a) a ciliate host cell, b) at least one cDNA encoding for a viral protein, or a fragment thereof, and c) a promoter operably linked to said cDNA

Abstract: A method characterizes samples having units by monitoring fluctuating intensities of radiation emitted, scattered, and/or reflected by the units in at least one measurement volume, the monitoring being performed by at least one detection device, the method comprising the steps of: a) measuring in a repetitive mode a number of photon counts per time interval of defined length, b) determining a function of the number of photon counts per the time interval, and c) determining a function of specific brightness of the units on basis of the function of the number of photon counts.

Abstract: A method for producing a ciliate cell with reduced or essentially no dihydrofolate reductase (DHFS) activity or reduced or essentially no thymidylate synthase (TS) activity or both reduced or essentially no dihydrofolate reductase and thymidylate synthase (DHFR-TS) activity is claimed, comprising the steps of a) transforming ciliate cells by inserting a construct containing an allele altering the gene encoding the endogenous DHFR-TS into at least one of the endogenous DHFR-TS genes of the ciliate macronucleus (MAC), b) inducing an allelic assortment process in the transformed ciliate cells to generate cells having the construct inserted in most or all functional DHFR-TS genes of the MAC, and c) identifying the cells generated in step b) by cultivation with or without thymidine.

Abstract: A medicament containing enzymes from ciliates selected from the group consisting of hydrolases, lipases, proteases, amylases, glycosidases, phospholipases, phosphodiesterases, phosphatases.

Abstract: The present invention relates to heat-inducible promoters of the heat shock protein family of the ciliate Tetrahymena thermophila, especially a promoter having the nucleotide sequence of Seq. ID No. 1 or promoter-effective fragments thereof. Furthermore the use of heat-inducible promoters of the present invention for the expression of homologous and/or heterologous proteins in the ciliate Tetrahymena thermophila is claimed.

Abstract: The invention relates to a method for identifying a protease secretion deficient strain of a microorganism, wherein: a mutant of the ciliate is added to a gel; and is incubated under conditions under which the mutant of the ciliate secretes proteins; said mutant of the ciliate is separated from the gel; wherein at least one substrate for at least one secretion protease of the ciliate is contained in said gel and/or said gel itself is the substrate and/or the substrate is added later and will at least in part diffuse into the gel; and the protease activity that has acted on the substrate is measured.

Abstract: The present invention is based on the finding that a capacitance can be measured precisely and efficiently when, in a delta-sigma modulator having an operational amplifier, a first capacitor connectable to an input of the operational amplifier, and a second capacitor in a feedback branch of the operational amplifier, a reference signal source is connectable to the first capacitor, wherein the first or second capacitor may represent a capacitance to be measured. Due to the fact that, in contrast to what is conventional, no input quantity is measured and digitalized at the input of the delta-sigma modulator, but instead a defined reference signal source is coupled at the input and a device of the delta-sigma modulator itself represents the measuring quantity, an extremely compact circuit is provided allowing capacitances to be measured quickly and reliably, the measuring result being additionally made available in a digital form.

Abstract: A directional coupler, for example a rat-race coupler, for use in radar engineering is disclosed. In one embodiment, the directional coupler includes at least three ports which are electrically connected to one another by a number of line branches, all line branches being constructed as balanced pairs of lines.

Abstract: A regulatory element of a DNA for an efficient heterologous expression of proteins in Tetrahymena ssp which efficient heterologous expression is performed under control of promotors and/or terminators which are derived from in Tetrahymena ssp naturally occurring DNA comprising promotors and/or terminators and a coding region for proteins secreted ion a high level and the expression of proteins secreted on a high level is independent of the cell-cycle of Tetrahymena ssp.

Abstract: A nucleic acid coding for the phospholipase A1 from ciliates. In particular, the phospholipase A1 has the amino acid sequence SEQ ID No. 7.

Abstract: A nucleic acid coding for the phospholipase A1 from ciliates. In particular, the phospholipase A1 has the amino acid sequence SEQ ID No. 7.