Abstract: The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.

Abstract: The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.

Abstract: The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.

Abstract: The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.

Abstract: The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.

Abstract: The invention provides a method of producing iPS cells, which comprises the steps of (i) introducing reprogramming factors into somatic cells; (ii) culturing the cells obtained in step (i) for more than 11 days and not more than 29 days; (iii) sorting TRA-1-60-positive cells from the cells obtained in step (ii); (iv) culturing the TRA-1-60-positive cells sorted in step (iii); (v) transferring a colony obtained in step (iv) to another culture vessel; and (vi) culturing the cells obtained in step (v), thereby obtaining iPS cells. The cells obtained in step (v) are preferably subcultured 10 times or more. The invention also provides a method of producing a population of differentiated cells that has a reduced rate of residual undifferentiated cells, which comprises inducing differentiation of the iPS cells obtained by the above-mentioned method.

Abstract: The present invention provides a method for selecting human induced pluripotent stem (iPS) cells which can be safely used for transplantation. That is, the present invention provides a method for selecting human iPS cells having reduced differentiation resistance, comprising the steps of: (1) inducing differentiation of human iPS cells; (2) detecting remaining undifferentiated cells after the step (1); and (3) selecting human iPS cells whose rate of remaining undifferentiated cells detected in step (2) is equivalent to or not more than that of control cells.

Abstract: Provided is a method of selecting highly safe pluripotent stem cells that do not exhibit differentiation resistance, comprising the steps of (1) inducing a pluripotent stem cell to differentiate, (2) culturing the cell under conditions for maintaining undifferentiated state, (3) detecting the generation of an undifferentiated cell by the cultivation, and comparing the finding with a control, and (4) selecting a pluripotent stem cell whose detected value is not more than a control generation value.

Abstract: The present invention provides a method for screening for iPS cells exhibiting differentiation resistance using a marker identified as lincRNA or mRNA that is specifically expressed in an iPS cell line exhibiting differentiation resistance, and such markers.

Abstract: The present invention provides a method for selecting human induced pluripotent stem (iPS) cells which can be safely used for transplantation. That is, the present invention provides a method for selecting human iPS cells having reduced differentiation resistance, comprising the steps of: (1) inducing differentiation of human iPS cells; (2) detecting remaining undifferentiated cells after the step (1); and (3) selecting human iPS cells whose rate of remaining undifferentiated cells detected in step (2) is equivalent to or not more than that of control cells.

Abstract: Provided is a method of selecting highly safe pluripotent stem cells that do not exhibit differentiation resistance, comprising the steps of (1) inducing a pluripotent stem cell to differentiate, (2) culturing the cell under conditions for maintaining undifferentiated state, (3) detecting the generation of an undifferentiated cell by the cultivation, and comparing the finding with a control, and (4) selecting a pluripotent stem cell whose detected value is not more than a control generation value.