Patents by Inventor Marilyn E. Smith

Marilyn E. Smith has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Purified mixture of collagenase I, collagenase II and two other proteases
    Patent number: 5989888
    Abstract: A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases, and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cell clusters from tissue. Finally, a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and recover cells or cell clusters.
    Type: Grant
    Filed: March 13, 1998
    Date of Patent: November 23, 1999
    Assignee: Roche Diagnostics Corporation
    Inventors: Francis E. Dwulet, Bernice B. Ellis, John F. Gill, Linda B. Jacobsen, Marilyn E. Smith, David G. Waters
  • Enzyme composition for tissue dissociation
    Patent number: 5952215
    Abstract: An enzyme composition and its use for isolating cells or cell clusters from tissue is disclosed. The composition includes two purified collagenase enzymes and an endoprotease. The ratio of the total FITC casein activity of the enzyme composition to the Wunsch units of activity of the masses of collagenase I and collagenase II is about 85:1 to about 3,900:1.
    Type: Grant
    Filed: November 2, 1998
    Date of Patent: September 14, 1999
    Assignee: Roche Diagnostics Corporation
    Inventors: Francis E. Dwulet, Marilyn E. Smith
  • Composition for tissue dissociation containing collagenase I and II from clostridium histolyticum and a neutral protease
    Patent number: 5830741
    Abstract: An enzyme composition for dissociating tissues is disclosed. The composition comprises purified collagenase I and collagenase II from Clostridium histolyticum in a mass ratio of about 0.3 to 0.6 and a neutral protease such that the ratio of total FITC casein units of activity to Wunsch units of activity in the composition is about 250:1 to about 600:1. The preferred neutral protease is thermolysin.
    Type: Grant
    Filed: December 6, 1996
    Date of Patent: November 3, 1998
    Assignee: Boehringer Mannheim Corporation
    Inventors: Francis E. Dwulet, Marilyn E. Smith
  • Purified mixture of collagenase I, collagenase II and two other proteases
    Patent number: 5753485
    Abstract: A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases, and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cell clusters from tissue. Finally, a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and recover cells or cell clusters.
    Type: Grant
    Filed: January 24, 1996
    Date of Patent: May 19, 1998
    Assignee: Boehringer Mannheim Corporation
    Inventors: Francis E. Dwulet, Bernice B. Ellis, John F. Gill, Linda B. Jacobsen, Marilyn E. Smith, David G. Waters