Abstract: A method for testing a sample for the presence of one or more targets comprises multiplexed catalyzed reporter deposition (CARD) is provided.

Abstract: A method for testing a sample for the presence of one or more targets comprises multiplexed catalyzed reporter deposition (CARD) is provided.

Abstract: Methods for multiplexed target detection are disclosed. Signal amplification in multiplexed solid-phase phase target detection methods are disclosed.

Abstract: Methods for multicolor multiplex imaging are provided herein in order to increase the number of targets that may be imaged given the number of fluorophores available or desired for use.

Abstract: A method for testing a sample for the presence of one or more targets comprises multiplexed catalyzed reporter deposition (CARD) is provided.

Abstract: Disclosed herein are methods and compositions for evaluating genomic content using encoded particles to evaluate multiple samples in parallel.

Abstract: The present invention concerns an array-based analytical system and method having an enhanced sensitivity which allows for simple and rapid analysis of relative unmodified samples which comprises an analytical system of the type having a plurality of different first members of a specific binding pair affixed in an array thereupon, a mixture including at least one second member of a specific binding pair capable of binding to one of the first members so as to form a specific binding pair which is affixed to the support member, and a reporter system that produces a detectable signal indicative of the presence of the specific binding pair on the support member and wherein the reporter system includes an amplified reporter system that is independent of layering.

Abstract: Disclosed herein are methods and compositions for multiplexed assays using magnetic and non-magnetic particles. Also disclosed are methods and compositions for performing multi-step assays within a single assay vessel.

Abstract: This invention relates to methods and compositions useful in detecting enzymatic activity. Also featured are methods for diagnosing enzyme deficiencies such as metabolic disorders.

Abstract: Disclosed herein are methods and compositions for evaluating genomic content using encoded particles to evaluate multiple samples in parallel.

Abstract: Disclosed are compounds of the general formulas: wherein Y is a moiety capable of being cleaved by a hydrolytic enzyme; L is a detectable label; X is a linking group; Z is a halogen; and R is hydrogen, an alkyl, or a halogen. Compounds of this general formula can be used as substrates in an analyte-dependent enzyme activation system, such as that employed in connection with catalyzed reporter depositions. Also disclosed are assays using the compounds, and kits for carrying out the assays.

Abstract: The methods described herein can be used, e.g., for detecting at least two target molecules in a sample (e.g., a single sample). Exemplary methods include using a target-specific switch probe attached to a substrate and using zip codes to associate holographically encoded particles with a target-specific probe.

Abstract: A system and method for developing and utilizing particle-based n-multiplexed assays that include three or more reporters utilizes n particle sets that are associated with particle identification images or labels (IDs) that differ between sets. The encoded particles for a given set are coated with a specific binding member, or in the case of the sandwich assay with coupled capture and detector binding pair members, to form particle types. The sets of particle types are then pooled, and aliquots of the particle types are removed to assay vessels. Next, samples with three or four reporter molecules are supplied to the respective vessels. After one or more incubation periods, the particles are supplied to a reader system, which determines the particle IDs to identify the particle types and also detects the reporter signals. The reader system includes multiple excitation lasers that excite the various reporters in sequence or in parallel, to supply associated signals to one or more detectors.

Abstract: The present invention provides systems, methods and kits for performing multiplexed assays for glycoproteins using encoded particles as supports for glycoprotein specific binding agents.

Abstract: The invention provides an apparatus for interrogating an optical biosensor. The apparatus includes (a) locating means for positioning one or more optical biosensors; (b) calibration means for generating a reference measurement; (c) one or more biosensors for generating one or more sample measurements, the biosensors being substantially planar and removable from the apparatus; (d) a reading means for interrogating biosensor and calibration means, and (e) a transport means for moving one or more of a biosensor, the calibration means and the reading means, whereby the reading means is positioned to interrogate the biosensor and the calibration means.

Abstract: The present invention describes rhodamine compounds particularly well-suited for pre-labeling a protein that is then subjected to two-dimensional electrophoresis. Isomeric purification and synthesis of a dye having net neutral charge or no charge precludes interference with isoelectric electrophoretic separation.