Patents by Inventor Mark Cigan
Mark Cigan has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20180298397Abstract: Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.Type: ApplicationFiled: March 23, 2018Publication date: October 18, 2018Inventors: ANDREW MARK CIGAN, SAVERIO CARL FALCO, HUIRONG GAO, MICHAEL W. LASSNER, ZHONGSEN LI, ZHAN-BIN LIU, LESZEK A. LYZNIK, DAVID J. PETERSON, CHRISTOPHER JAY SCELONGE
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Publication number: 20180282763Abstract: Compositions and methods are provided for restoring function to a non-functional gene product in the genome of a cell. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to restore function to a non-functional gene product and to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. The present disclosure also describes methods for modifying a nucleotide sequence in the genome of a cell using a restored functional selectable marker, as well as methods for editing a nucleotide sequence in the genome a cell without introducing a polynucleotide modification template into said cell. Compositions and methods are also provided for DNA free delivery of Cas endonucleases, sgRNAs and guide RNA/Cas complexes.Type: ApplicationFiled: October 17, 2016Publication date: October 4, 2018Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, SERGEI SVITASHEV
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Publication number: 20180273960Abstract: Compositions and methods are provided for modifying a nucleotide sequence in the genome of a plant cell, without the use of a selectable marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to make a double strand break in a target site located in a nucleotide sequence and plant cells are obtained without the use of a selectable marker, and to provide an effective system for modifying target sites within the genome of a plant, plant cell or seed. Compositions and methods are also provided for producing a plant cell, callus tissue or plant having a modified nucleotide sequence in its genome, without the use of a selectable marker.Type: ApplicationFiled: October 17, 2016Publication date: September 27, 2018Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, SERGEI SVITASHEV
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Publication number: 20180258438Abstract: Compositions and methods are provided producing a complex trait locus in a genomic window of a soybean plant comprising (i) at least one transgenic target site for site specific integration integrated in at least double-strand-break target site, (ii) at least one double-strand-break target site and at least one transgene, (iii) at least one altered double-strand-break target site or (iv) any one combination of (i)-(iii). The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.Type: ApplicationFiled: October 27, 2016Publication date: September 13, 2018Applicants: PIONEER HI-BRED INTERNATIONAL, INC., E. I. DU PONT DE NEMOURS AND COMPANYInventors: JULIAN MARCO CHAKY, ANDREW MARK CIGAN, ANTHONY J. KINNEY, ZHAN-BIN LIU, JOHN BRYAN WOODWARD, JOSHUA K. YOUNG
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Publication number: 20180258417Abstract: Compositions and methods are provided for rapid characterization of Cas endonuclease systems and the elements comprising such systems, including, but not limiting to, rapid characterization of PAM sequences, guide RNA elements and Cas endonucleases. Type II Cas9 endonuclease systems originating from Brevibacillus laterosporus, Lactobacillus reuteri MIc3, Lactobacillus rossiae DSM 15814, Pediococcus pentosaceus SL4, Lactobacillus nodensis JCM 14932, Sulfurospirillum sp. SCADC, Bifidobacterium thermophilum DSM 20210, Loktanella vestfoldensis, Sphingomonas sanxanigenens NX02, Epilithonimonas tenax DSM 16811, Sporocytophaga myxococcoides are described herein. The present disclosure also describes methods for genome modification of a target sequence in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell.Type: ApplicationFiled: May 12, 2016Publication date: September 13, 2018Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, GIEDRIUS GASIUNAS, TAUTVYDAS KARVELIS, VIRGINIJUS SIKSNYS, JOSHUA K YOUNG
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Publication number: 20180230476Abstract: Compositions and methods are provided for introducing transgenic target sites for Site Specific Integration (SSI) and/or polynucleotides of interest into at least one double-strand break target site of a double-strand-break inducing agent in a genomic window of a plant genome. Also provided are methods and compositions for producing a complex trait locus in a genomic window of a plant comprising at least one transgenic target site for site specific integration integrated in at least double-strand-break target site. The double-strand-break target site can be, but is not limited to, a target site for a zinc finger endonuclease, an engineered endonuclease, a meganuclease, a TALENs and/or a Cas endonuclease. The genomic window of said plant can comprise at least one genomic locus of interest such as a trait cassette, a transgene, a mutated gene, a native gene, an edited gene or a site-specific integration (SSI) target site.Type: ApplicationFiled: August 31, 2015Publication date: August 16, 2018Inventors: ANDREW MARK CIGAN, HUIRONG GAO, ZHAN-BIN LIU, JASDEEP MUTTI, DEAN PODLICH, CHRISTOPHER JAY SCELONGE
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Patent number: 10030245Abstract: Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.Type: GrantFiled: March 22, 2012Date of Patent: July 24, 2018Assignees: E I DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Michael Lassner, Derek Jantz, James Jefferson Smith, Mark Cigan, Carl Falco, Huirong Gao, Zhongsen Li, Zhan-Bin Liu, Sergei Svitashev
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Publication number: 20180201946Abstract: Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants, and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are methods for testing allelic variants.Type: ApplicationFiled: March 7, 2018Publication date: July 19, 2018Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, ERICA UNGER-WALLACE
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Publication number: 20180142223Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.Type: ApplicationFiled: January 10, 2018Publication date: May 24, 2018Applicants: E. I. DUPONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ERICKA BERMUDEZ, ANDREW MARK CIGAN, JAMES J ENGLISH, SAVERIO CARL FALCO, HUIRONG GAO, LU LIU, ZHAN-BIN LIU, AZALEA S ONG, SERGEI SVITASHEV, JOSHUA K YOUNG
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Patent number: 9951346Abstract: Compositions and methods are provided for the targeted integration of a polynucleotide sequence of interest into the genome of a plant or plant cell. The methods and compositions employ recognition sites for endonucleases and endonucleases in combination with site-specific recombination sites/recombinases to provide an effective system for establishing target sites within the genome of a plant, plant cell or seed. Once such target sites are established, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest.Type: GrantFiled: July 18, 2012Date of Patent: April 24, 2018Assignees: Pioneer Hi-Bred International, Inc., E. I. du Pont de Nemours and CompanyInventors: Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Michael W. Lassner, Zhongsen Li, Zhan-Bin Liu, Leszek A. Lyznik, David J. Peterson, Christopher Jay Scelonge
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Patent number: 9909110Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.Type: GrantFiled: October 11, 2016Date of Patent: March 6, 2018Assignees: E. I. DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL, INC.Inventors: Ericka Bermudez, Andrew Mark Cigan, James English, Saverio Carl Falco, Huirong Gao, Lu Liu, Zhan-Bin Liu, Azalea Ong, Sergei Svitashev, Joshua K Young
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Publication number: 20180010145Abstract: Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part. In certain embodiments, the plant is polyploid.Type: ApplicationFiled: September 26, 2017Publication date: January 11, 2018Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventor: ANDREW MARK CIGAN
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Publication number: 20180002715Abstract: Compositions and methods are provided for regulated expression of a guide RNA/Cas endonuclease system in a plant cell, plant and seed. Compositions and methods are also provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a regulated guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed.Type: ApplicationFiled: February 15, 2016Publication date: January 4, 2018Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, SERGEI SVITASHEV
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Publication number: 20170369902Abstract: Manipulation of male fertility in a polyploid species requires attention to the interaction of male-fertility alleles of multiple genomes. In hexaploid wheat, single-genome heterozygotes for Ms26 provide differential levels of male fertility across genomes. Hexaploid wheat homozygous for mutations in the Ms26 gene on the A, B, and D genomes is male-sterile. Male fertility may be restored by sufficient levels of expression of Ms26 using native Ms26 or a transgene, which may be native to wheat or to another species, or a combination of native and transgenic alleles. CRISPR/Cas9 technology may be used to generate mutations in Ms26 in wheat or rice.Type: ApplicationFiled: December 15, 2015Publication date: December 28, 2017Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, MANJIT SINGH
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Publication number: 20170342430Abstract: Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are methods for maintaining a homozygous recessive condition and for repressing transmission of transgenes.Type: ApplicationFiled: August 10, 2017Publication date: November 30, 2017Applicant: PIONEER HI-BRED INTERNATIONAL, INC.Inventors: ANDREW MARK CIGAN, TIM FOX, HOWARD P HERSHEY, ERICA UNGER-WALLACE, YONGZHONG WU
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Patent number: 9803214Abstract: Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part. In certain embodiments, the plant is polyploid. Compositions and methods using promoter inverted repeats to modulate male sterility in plants are provided as well as compositions and methods for restoring fertility.Type: GrantFiled: March 11, 2014Date of Patent: October 31, 2017Assignee: PIONEER HI-BRED INTERNATIONAL, INC.Inventor: Andrew Mark Cigan
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Publication number: 20170298383Abstract: Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.Type: ApplicationFiled: September 21, 2015Publication date: October 19, 2017Inventors: MARC C. ALBERTSEN, UTE BAUMANN, ANDREW MARK CIGAN, MANJIT SINGH, ELISE TUCKER, RYAN WHITFORD
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Publication number: 20170058295Abstract: Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.Type: ApplicationFiled: October 11, 2016Publication date: March 2, 2017Inventors: DENNIS L. BIDNEY, Andrew Mark Cigan, Saverio Carl Falco, Huirong Gao, Derek Jantz, Michael Lassner, Keith S. Lowe, Leszek Aleksander Lyznik, James Jefferson Smith
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Patent number: 9574208Abstract: Methods of making a targeted modification in a male fertility gene in the genome of a plant are disclosed. The methods involve contacting a plant cell with an engineered double-strand-break-inducing agent capable of inducing a double-strand break in a target sequence in the male fertility gene and identifying a cell comprising an alteration in the target sequence. Also disclosed are plants, plant cells, plant parts, and seeds comprising a male fertility gene with an alteration in a male fertility gene. Nucleic acid molecules comprising male fertility genes with at least one targeted modification therein, optimized nucleic acid molecules encoding endonucleases that are engineered double-strand-break-inducing agents and expression cassettes, host cells, and plants comprising one or more of the nucleic acid molecules are further disclosed.Type: GrantFiled: June 19, 2012Date of Patent: February 21, 2017Assignees: EI DU PONT DE NEMOURS AND COMPANY, PIONEER HI-BRED INTERNATIONAL INCInventors: Dennis L. Bidney, Mark Cigan, Carl Falco, Huirong Gao, Derek Jantz, Mike Lassner, Keith Lowe, Leszek Aleksander Lyznik, James Jefferson Smith
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Publication number: 20170022488Abstract: Compositions and methods comprising polynucleotides and polypeptides having meganuclease activity are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the meganuclease sequences. Various methods of employing the meganuclease sequences are provided. Such methods include, for example, methods for producing a meganuclease with increased activity at a wide range of temperatures, methods for producing a yeast, plant, plant cell, explant or seed comprising a meganuclease with increased activity.Type: ApplicationFiled: October 11, 2016Publication date: January 26, 2017Inventors: ERICKA BERMUDEZ, ANDREW MARK CIGAN, JAMES ENGLISH, CARL FALCO, HUIRONG GAO, LU LIU, ZHAN-BIN LIU, AZALEA ONG, SERGEI SVITASHEV, JOSHUA K YOUNG