Abstract: The present disclosure relates to designs and applications of Variable Heavy Only (VHO) domain regions. The present disclosure provides a VHO library of polynucleotides encoding VHO domains, wherein the VHO domains are designed based on the Vh domain of a human Vh family, and a method of generating the VHO library. The present disclosure also provides a phage library displaying the VHO domains encoded by the VHO library through the use of M13 bacteriophage minor coat proteins such as pIX and pVII and a method of generating the phage library. The present disclosure also provides a method of screening the phage library to identify VHO candidates that are capable of binding a target of interest.

Abstract: An apparatus and method for dispensing protein-containing materials, such as, for example, pastes, gels, lipidic cubic phases, lipidic mesophases, and mixtures of the foregoing into containers, e.g., wells of assay trays. The invention further provides a method for preparing the aforementioned containers for various experiments involving soluble proteins or membrane proteins or both types of proteins. In a preferred embodiment, the apparatus includes (a) a gel dispenser assembly for dispensing small quantities of gels; (b) a pipette probe for dispensing fluids; (c) an actuator for moving the gel dispenser assembly vertically relative to the pipette probe; (d) a liquid-handling robot having a robot arm for moving the pipette probe and the gel dispenser assembly vertically and horizontally relative to containers, e.g.

Abstract: An apparatus and method for dispensing protein-containing materials, such as, for example, pastes, gels, lipidic cubic phases, lipidic mesophases, and mixtures of the foregoing into containers, e.g., wells of assay trays. The invention further provides a method for preparing the aforementioned containers for various experiments involving soluble proteins or membrane proteins or both types of proteins. In a preferred embodiment, the apparatus includes (a) a gel dispenser assembly for dispensing small quantities of gels; (b) a pipette probe for dispensing fluids; (c) an actuator for moving the gel dispenser assembly vertically relative to the pipette probe; (d) a liquid-handling robot having a robot arm for moving the pipette probe and the gel dispenser assembly vertically and horizontally relative to containers, e.g.