Patents by Inventor Markus Klintstedt
Markus Klintstedt has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220195490Abstract: The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.Type: ApplicationFiled: January 19, 2022Publication date: June 23, 2022Applicant: Q-linea ABInventors: Markus Klintstedt, Harer Osman, Mats Gullberg
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Patent number: 11261476Abstract: The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.Type: GrantFiled: October 19, 2017Date of Patent: March 1, 2022Assignee: Q-LINEA ABInventors: Markus Klintstedt, Harer Osman, Mats Gullberg
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Publication number: 20210032674Abstract: The present invention provides a method of preparing a suspension of intact microorganisms from a sample containing microorganisms and mammalian cells, comprising contacting the sample with a buffer solution with a pH of at least pH 6 and less than pH 9, a detergent and one or more proteases to allow lysis of the mammalian cells; filtering the mixture through a filter suitable for retaining microorganisms to remove the lysed mammalian cells; resuspending the microorganisms retained by the filter in a liquid to provide a suspension comprising the recovered intact microorganisms; and determining the concentration of microorganisms in the suspension by: i. heating and/or contacting an aliquot of the suspension with an alcohol; ii. optionally diluting one or more aliquots of the suspension to provide one or more diluted aliquots before, during and/or after step (i); iii. contacting at least a portion of an aliquot of step (i) or (ii) with a single fluorescent stain capable of binding to DNA; iv.Type: ApplicationFiled: January 22, 2019Publication date: February 4, 2021Applicant: Q-LINEA ABInventors: Jan GRAWÉ, Markus KLINTSTEDT
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Publication number: 20210017568Abstract: The present invention provides methods for determining the antimicrobial susceptibility of a microorganism in a clinical sample said method comprising removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units, isolating the microbial cells and transferring the cells into a suitable culture medium for microbial growth, and performing an AST assay using the isolated microbes, wherein the concentration of microbial cells in the microbial cells used to set up the AST assay is measured before the degree of microbial growth in the different growth conditions of the AST assay is measured. Devices for determining the antimicrobial susceptibility of a microorganism in a clinical sample are also provided.Type: ApplicationFiled: October 1, 2020Publication date: January 21, 2021Applicant: Q-linea ABInventors: Jonas Jarvius, Jan Grawe, Ylva Molin, Markus Klintstedt, Mats Gullberg
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Patent number: 10829797Abstract: A method for determining the antimicrobial susceptibility of a microorganism in a clinical sample includes removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units and isolating the microbial cells. The cells are transferred into a suitable culture medium for microbial growth and an AST assay is performed using the isolated microbes. The concentration of microbial cells in the microbial cells used to set up the AST assay is measured before measuring the degree of microbial growth in different conditions of the AST assay. Devices for determining the anti-microbial susceptibility of a microorganism in a clinical sample are also disclosed.Type: GrantFiled: June 16, 2016Date of Patent: November 10, 2020Assignee: Q-linea ABInventors: Jonas Jarvius, Jan Grawe, Ylva Molin, Markus Klintstedt, Mats Gullberg
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Publication number: 20200299748Abstract: The present invention relates to a method of determining the concentration of intact microorganisms in a sample comprising optionally diluting an aliquot of the sample to provide a diluted aliquot at a dilution value; contacting at least a portion of an aliquot or of a diluted aliquot of the sample with first and second stains capable of binding to DNA, wherein the first stain is a fluorescent stain, is cell-permeable, and has a first emission wavelength, and the second stain is cell-impermeable, and is capable of acting as an acceptor molecule in a FRET pair with the first stain acting as a donor molecule; imaging the aliquot-stain mixture at the first emission wavelength and determining an image analysis value for the number of objects corresponding to intact microorganisms in the imaged mixture; and comparing the image analysis value for said aliquot to a pre-determined calibration curve, as well as an apparatus, a consumable and a kit therefor.Type: ApplicationFiled: October 12, 2018Publication date: September 24, 2020Applicant: Q-LINEA ABInventors: Jonas JARVIUS, Jan GRAWE, Markus KLINTSTEDT
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Publication number: 20190316173Abstract: The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.Type: ApplicationFiled: October 19, 2017Publication date: October 17, 2019Applicant: Q-linea ABInventors: Markus Klintstedt, Harer Osman, Mats Gullberg
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Publication number: 20180223327Abstract: The present invention provides methods for determining the antimicrobial susceptibility of a microorganism in a clinical sample said method comprising removing a test aliquot from a clinical sample culture before the culture reaches 0.5 McFarland units, isolating the microbial cells and transferring the cells into a suitable culture medium for microbial growth, and performing an AST assay using the isolated microbes, wherein the concentration of microbial cells in the microbial cells used to set up the AST assay is measured before the degree of microbial growth in the different growth conditions of the AST assay is measured. Devices for determining the antimicrobial susceptibility of a microorganism in a clinical sample are also provided.Type: ApplicationFiled: June 16, 2016Publication date: August 9, 2018Applicant: Q-linea ABInventors: Jonas Jarvius, Jan Grawe, Ylva Molin, Markus Klintstedt, Mats Gullberg
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Patent number: D902428Type: GrantFiled: April 20, 2018Date of Patent: November 17, 2020Assignee: Q-linea ABInventors: Markus Klintstedt, Peter Olsson, Anders Lind, Simon Uhrberg, Jerker Persson, Richard Kroon
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Patent number: D950769Type: GrantFiled: October 11, 2019Date of Patent: May 3, 2022Assignee: Q-LINEA ABInventors: Markus Klintstedt, Peter Olsson, Anders Lind, Simon Uhrberg, Richard Kroon, Jerker Persson, Charlotta Goransson, Emma Rennel Dickens