Abstract: The present invention relates to the field of diagnostic methods. Specifically, the present invention contemplates a method for differentiating in a subject between heart failure and pulmonary disease based on determining the amounts of at least one biomarker. The invention also relates to tools for carrying out the aforementioned methods, such as diagnostic devices.

Abstract: Provided herein is a method for determining a metabolic state of a plant or part thereof comprising a) rapid evaporating a multitude of metabolites of said plant or part thereof; b) determining the amount of at least one metabolite characteristic of said metabolic state; and c) thereby, determining a metabolic state of a plant thereof. Further provided is a method for in vivo determining a metabolite distribution in a plant or part thereof comprising a) in vivo rapid evaporating at least one metabolite of interest in at least a first and a second location of said plant or part thereof; b) determining the amounts of at least one metabolite at said first and a second location; and, c) thereby, in vivo determining metabolite distribution in a plant or part thereof. Moreover, provided are devices, data collections, and uses relating to the aforesaid methods.

Abstract: The present invention relates to the field of diagnostic methods. Specifically, the present invention contemplates a method for diagnosing a cardiac disease in a subject based on determining the amounts of at least three lipid metabolite biomarkers and at least one further cardiac biomarker. The invention also relates to tools for carrying out the aforementioned methods, such as diagnostic devices.

Abstract: The present invention relates to a method for diagnosing pancreatic cancer in a subject comprising the steps of: (a) determining in at least one sample of said subject the amounts of a group of diagnostic biomarkers comprising (i) at least one diagnostic amino acid, said diagnostic amino acid being proline, histidine or tryptophan, preferably, being proline; (ii) at least one diagnostic ceramide, said diagnostic ceramide being ceramide (d18:1,C24:0) or ceramide (d18:2,C24:0), preferably being ceramide (d18:1,C24:0); (iii) at least one diagnostic sphingomyelin, said diagnostic sphingomyelin being sphingomyelin (35:1), sphingomyelin (d17:1,C16:0), sphingomyelin (41:2) or sphingomyelin (d18:2,C17:0), preferably being sphingomyelin (35:1); and (iv) CA19-9; and (b) comparing said amounts of the diagnostic biomarkers with a reference, whereby pancreatic cancer is diagnosed.

Abstract: The present invention relates to the field of diagnostic methods. Specifically, the present invention contemplates a method for diagnosing heart failure in a subject based on determining the amounts of at least three biomarkers. The invention also relates to tools for carrying out the aforementioned methods, such as diagnostic devices.

Abstract: The present invention provides a method for profiling phytohormone levels in plant tissue or tissue of other plastid containing organisms, i.e. a method for the simultaneous determination of a multitude of phytohormone levels in plant tissue or tissue of other plastid containing organisms.

Abstract: The present invention relates to the field of diagnostic methods. Specifically, the present invention relates to a method for assessing the quality of a biological sample comprising the steps of determining in a sample the amount of at least one biomarker from Tables 1, 1?, 2, 2?, 3, 3?, 4, 5, 5?, 6, 7, and/or 8 and comparing the said amount of the at least one biomarker with a reference, whereby the quality of the sample is assessed. The invention also relates to tools for carrying out the aforementioned method, such as devices and kits.

Abstract: The present invention provides a method for profiling phytohormone levels in plant tissue or tissue of other plastid containing organisms, i.e. a method for the simultaneous determination of a multitude of phytohormone levels in plant tissue or tissue of other plastid containing organisms, which method comprises the following steps: i. extraction of particulate tissue material of the plastid containing organism, in particulate tissue material of plants, with a liquid extractant, which is a mixture of at least one water miscible neutral organic solvent having from 1 to 3 carbon atoms and 1 heteroatom selected from O and N with water containing from 0.1 to 5% by weight, based on the extractant, of at least one acid, whereby a first liquid extract is obtained; ii. contacting the liquid extract obtained in step i. with a solid absorbent having a hydrophobically modified surface and removing the solid absorbent to obtain a second liquid extract; iii.

Abstract: The present invention is concerned with the analysis of polar metabolites and provides methods for analyzing polar metabolites including extracting a biological sample with a extraction buffer containing a phase separator and a volatile neutral ammonium salt under conditions which allow for immediate disruption of cells in the biological sample, separating the polar metabolites in the extract by chromatography, and analyzing the separated polar metabolites. Moreover, a method for quenching a biological sample containing cellular material is contemplated.

Abstract: The present invention relates to a process for extracting constituents from organic material, comprising the step of extracting with a monophasic mixture of 50% by volume to 90% by volume of methanol and 50 to 10% by volume of water and optionally 0% by volume to 40% by volume of a further solvent or solvent mixture.

Abstract: A method for characterizing a sample containing at least one compound is disclosed. Initially, a three-dimensional set of data is generated through sample analysis using time resolved and mass resolved separation techniques. The data comprises at least one signal as a function of a mass variable over a first range of measurement and of a time variable over a second range of measurement. The first range of measurement is divided into at least two mass variable intervals, and for each mass variable interval, an extracted signal is selected which is a function of the time variable. The second range of measurement is divided into at least one time variable interval. A characteristic value is then selected for each time variable interval and each extracted signal to generate a characteristic sample profile, comprising one or more characteristic values as functions of respective time variable intervals and respective mass variable intervals.

Abstract: The present invention is concerned with the analysis of polar metabolites and provides methods for analyzing polar metabolites comprising extracting a biological sample with a ex-traction buffer comprising a phase separator and a volatile neutral ammonium salt under conditions which allow for immediate disruption of cells comprised by the biological sample, separating the polar metabolites comprised by the extract by chromatography, and analyzing the separated polar metabolites. Moreover, a method for quenching a biological sample comprising cellular material is contemplated.

Abstract: The present invention relates to a process for extracting constituents from organic material, comprising the step of extracting with a monophasic mixture of 50% by volume to 90% by volume of methanol and 50 to 10% by volume of water and optionally 0% by volume to 40% by volume of a further solvent or solvent mixture.

Abstract: The present invention relates to a process for extracting constituents from organic material, comprising the step of extracting with a monophasic mixture of 50% by volume to 90% by volume of methanol and 50 to 10% by volume of water and optionally 0% by volume to 40% by volume of a further solvent or solvent mixture.

Abstract: The present invention relates to a process for extracting constituents from organic material, comprising the step of extracting with a monophasic mixture of 50% by volume to 90% by volume of methanol and 50 to 10% by volume of water and optionally 0% by volume to 40% by volume of a further solvent or solvent mixture.

Abstract: The invention relates to a method for the extraction of components made from organic material, comprising the step of extraction with a single-phase mixture of 50 to 90 vol. % methanol and 50 to 10 vol. % water and optionally 0 to 40 vol. % of a further solvent or solvent mixture.

Abstract: A method for characterizing a sample containing at least one compound, preferably a biological sample comprising at least one metabolite, is proposed. In a first process step, a three-dimensional first set of data is generated by analyzing the sample by using at least one time resolved separation technique (214) and at least mass one resolved separation technique (216). The set of data comprises at least one signal I as a function of a mass variable over a first range of measurement (420) and of a time variable over a second range of measurement (422). In a second process step, the first range of measurement (420) is divided into at least two mass variable intervals (424). For each mass variable interval (424), an extracted signal is selected, wherein the extracted signal is a function of the time variable. In a third process step, the second range of measurement (422) is divided into at least one time variable interval (426).

Abstract: The present invention relates to a process for isolating constituents from organic material, comprising the following process steps: a) freeze-drying of the organic material; b) extracting the constituents with a polar solvent or solvent mixture (A) and an organic solvent or solvent mixture (B), it being possible for the extracts of the extraction with (A) and (B) to form one phase; c) combining the extracts (A) and (B) to give one phase; and d) carrying out an esterification/transesterification in the unpolar phase with an alcohol, the esterification/transesterification being carried out in the presence of a volatile acid; the process being a high-throughput process.

Abstract: The invention relates to a mass spectrometry method for analysing mixtures of substances using a triple quadrupole mass spectrometer, whereby said mixtures of substances are ionised prior to analysis.

Abstract: The present invention relates to a process for isolating constituents from organic material, comprising the following process steps: a) freeze-drying of the organic material; b) extracting the constituents with a polar solvent or solvent mixture (A) and an organic solvent or solvent mixture (B), it being possible for the extracts of the extraction with (A) and (B) to form one phase; c) combining the extracts (A) and (B) to give one phase; and d) carrying out an esterification/transesterification in the unpolar phase with an alcohol, the esterification/transesterification being carried out in the presence of a volatile acid; the process being a high-throughput process.