Abstract: A charge detection mass spectrometer may include an electrostatic linear ion trap (ELIT) or an orbitrap, an ion source to supply ions thereto, at least one amplifier operatively coupled to the ELIT or orbitrap, a processor coupled to ELIT or orbitrap and to the amplifier(s), and processor programmed to control the ELIT or orbitrap as part of a trapping event to attempt to trap therein a single ion supplied by the ion source, to record ion measurement information based on output signals produced by the amplifier(s) over a duration of the trapping event, to determine, based on the measurement information, whether the control of the ELIT or orbitrap resulted in trapping of a single ion, no ion or multiple ions, and to compute an ion mass or mass-to-charge ratio from the measurement information only if a single ion was trapped during the trapping event.

Abstract: A CDMS may include an ELIT having a charge detection cylinder (CD), a charge generator for generating a high frequency charge (HFC), a charge sensitive preamplifier (CP) having an input coupled to the CD and an output configured to produce a charge detection signal (CHD) in response to a charge induced on the CD, and a processor configured to (a) control the charge generator to induce an HFC on the CD, (b) control operation of the ELIT to cause a trapped ion to oscillate back and forth through the CD each time inducing a charge thereon, and (c) process CHD to (i) determine a gain factor as a function of the HFC induced on the CD, and (ii) modify a magnitude of the portion of CHD resulting from the charge induced on the CD by the trapped ion passing therethrough as a function of the gain factor.

Abstract: An interface transports ions from a first pressure environment to a lower pressure analysis instrument and may include a first region pumped to a second pressure less than the first pressure, a first ion funnel disposed in the first region, a first ion carpet in the first region opposite an ion outlet end of the first ion funnel, a second region pumped to a third pressure less than the second pressure and greater than the instrument pressure, a second ion funnel disposed in the second region and a second ion carpet in the second region opposite an ion outlet end of the second ion funnel. Ions from the environment pass sequentially through the first and second ion funnels and into the analysis instrument. Each of the first and second ion funnels define a tapered axial passageway therethrough each defining a respective virtual jet disrupter therein.

Abstract: An electrostatic linear ion trap has first and second axially aligned ion minors separated by a charge detection cylinder axially aligned with each ion minor. Electric fields are selectively established within the first and second ion minors in a manner which causes an ion in the trap to oscillate back and forth through the charge detection cylinder between the first and second ion minors with a duty cycle, corresponding to a ratio of time spent by the ion passing through the charge detection cylinder and total time spent traversing a combination of the first and second ion mirrors and the charge detection cylinder during one complete oscillation cycle, of approximately 50%.

Abstract: The present disclosure relates to a method of identifying components present in a lipoprotein. Methods provided include single particle mass spectrometry, such as charge detection mass spectrometry (CDMS). Distinct subpopulations that exist within lipoprotein classes are determined by correlating m/z and mass.

Abstract: The present invention provides a method of identifying components present in a preparation of virus particles, comprising: a) analyzing the preparation of virus particles with single molecule mass spectrometry to obtain a mass histogram; and b) interpreting the mass histogram of (a) to identify different components present in the preparation.

Abstract: A quadrupole mass filter and method for operating the filter are described. Sample ions, each having a mass-to-charge ratio, are passed to a quadrupole at least one AC voltage is applied thereto to separate the ions by incrementally varying a frequency of the at least one AC voltage within a first range of frequencies and, for each of at least some of the incremental frequencies in the first range of frequencies, incrementally varying an amplitude of the at least one AC voltage within a range of amplitudes, wherein each incremental frequency and incremental amplitude pair of the at least one AC voltage creates a different band pass filter in the quadrupole through which produced ions having a different corresponding mass-to-charge ratio pass to a detector.

Abstract: A quadrupole mass filter and method for operating the filter are described. Sample ions, each having a mass-to-charge ratio, are passed to a quadrupole at least one AC voltage is applied thereto to separate the ions by incrementally varying a frequency of the at least one AC voltage within a first range of frequencies and, for each of at least some of the incremental frequencies in the first range of frequencies, incrementally varying an amplitude of the at least one AC voltage within a range of amplitudes, wherein each incremental frequency and incremental amplitude pair of the at least one AC voltage creates a different band pass filter in the quadrupole through which produced ions having a different corresponding mass-to-charge ratio pass to a detector.

Abstract: The present invention provides a method of identifying components present in a preparation of virus particles, comprising: a) analyzing the preparation of virus particles with single molecule mass spectrometry to obtain a mass histogram; and b) interpreting the mass histogram of (a) to identify different components present in the preparation.

Abstract: A quadrupole mass filter and method for operating the filter are described. AC voltages are applied to the quadrupole to separate ions based on mass-to-charge ratio. Frequency is scanned with a simultaneous amplitude scan. Ions are measured over a broad m/z range with high resolution. A resolving power of about 1,200 was demonstrated. Ions were observed for m/z values over 150,000 Th.

Abstract: A quadrupole mass filter and method for operating the filter are described. AC voltages are applied to the quadrupole to separate ions based on mass-to-charge ratio. Frequency is scanned with a simultaneous amplitude scan. Ions are measured over a broad m/z range with high resolution. A resolving power of about 1,200 was demonstrated. Ions were observed for m/z values over 150,000 Th.