Abstract: A method for operating a bioprocess installation with an electronic process control and at least one bioprocess unit, wherein the bioprocess unit comprises a cell broth source with a first receptacle for cell broth including cultivation media and cells, establishing a culture environment for cell cultivation and/or bio production, wherein the bioprocess unit comprises a clarification setup with a centrifuge for the clarification of the cell broth by centrifugation, with a liquid pumping arrangement assigned to the centrifuge and with a liquid network with a number of liquid lines communicating with the liquid pumping arrangement, wherein out of a first culture environment established by the first receptacle, the cell broth is transfered to the centrifuge via the liquid network, which centrifuge is operated in a forward operation for cell separation and/or cell washing and in a backward operation for cell discharging.

Abstract: Methods, compositions, and kits are provided for CRISPR/Cas mediated transcriptional modulation.

Abstract: Methods, compositions, and kits are provided for CRISPR/Cas mediated transcriptional modulation.

Abstract: The present invention provides methods for conducting screens using nucleic acid elements (e.g., interfering RNAs) to confidently identify hit genetic elements. The present invention further comprises constructing vectors that contain two or more nucleic acid elements to knock down all pairwise combinations of the hit genetic elements identified from the screen. Following quantitation of the single and double-knockdown phenotypes, genetic interactions between all gene pairs can be calculated. Genes can then be clustered according to the similarity of the pattern of their interactions with all of the other genes to obtain a genetic interaction map, which can advantageously be used to predict functional associations between genes and identify drug targets for therapy such as combination cancer therapy.

Abstract: A method for treating contaminated liquids in a flow by ozone, wherein the ozone is fed in gas form into the flow at one point and then is mixed into the flow in stages by mixers following one another in the flow direction, so that an absolute quantity of introduced ozone increases after each mixer until a feed efficiency of more than 95 percent is reached.

Abstract: Methods, compositions, and kits are provided for CRISPR/Cas mediated transcriptional modulation.

Abstract: A method for treating contaminated liquids in a flow by ozone, wherein the ozone is fed in gas form into the flow at one point and then is mixed into the flow in stages by mixers following one another in the flow direction, so that an absolute quantity of introduced ozone increases after each mixer until a feed efficiency of more than 95 percent is reached.

Abstract: The present invention provides methods for conducting screens using nucleic acid elements (e.g., interfering RNAs) to confidently identify hit genetic elements. The present invention further comprises constructing vectors that contain two or more nucleic acid elements to knock down all pairwise combinations of the hit genetic elements identified from the screen. Following quantitation of the single and double-knockdown phenotypes, genetic interactions between all gene pairs can be calculated. Genes can then be clustered according to the similarity of the pattern of their interactions with all of the other genes to obtain a genetic interaction map, which can advantageously be used to predict functional associations between genes and identify drug targets for therapy such as combination cancer therapy.

Abstract: A device and a method for treating liquids by ozone, including a pipe in which a main flow of the liquid is guided in a flow direction from an inlet to an outlet, an ozone feed point at which ozone can be introduced in gas form into the main flow, and at least two mixers which are located downstream of the ozone feed point, wherein the mixers are arranged at a distance from one another, and wherein at least one reaction zone is formed between the mixers, and wherein the mixers are designed in such a way that they each dissolve only a portion of the ozone in the main flow that has been introduced in gas form.

Abstract: The invention relates to the oxidative treatment of bulk material in a vessel. The bulk material to be treated is placed in the vessel, and is humidified therein with water or an aqueous solution to such an extent that it can be treated with an O2/O3 gas mixture in such a way that the desired end product has a determined pH value or a determined solubility or a determined polarity or a determined solubility behaviour and/or particle size distribution. The inventive method enables bulk material to be very effectively oxidised by means of minimal amounts of ozone in an O2/O3 gas mixture, in such a way that between 70 and 100% of the ozone parts enter into a reaction.

Abstract: The invention relates to the oxidative treatment of bulk material in a vessel. The bulk material to be treated is placed in the vessel, and is humidified therein with water or an aqueous solution to such an extent that it can be treated with an O2/O3 gas mixture in such a way that the desired end product has a determined pH value or a determined solubility or a determined polarity or a determined solubility behaviour and/or particle size distribution. The inventive method enables bulk material to be very effectively oxidised by means of minimal amounts of ozone in an O2/O3 gas mixture, in such a way that between 70 and 100% of the ozone parts enter into a reaction.