Abstract: The present invention relates to a perfusion loop assembly for an ex vivo liver perfusion including: a pump for providing a fluid flow of a perfusion fluid through a first branch line and a second branch line; the first branch line being configured to provide a first portion of the perfusion fluid to the hepatic artery of the liver; the first branch line being coupled with a first gas exchanger, the second branch line being configured to provide a second portion of the perfusion fluid to the portal vein of the liver; the second branch line further including a first valve for controlling the flow of the perfusion fluid into the portal vein of the liver, a liver chamber assembly configured to hold the liver ex vivo, a liver outlet line attached to the vena cava of the ex vivo liver, at least one reservoir connected to the liver outlet and upstream from the pump.

Abstract: It is provided a method for evaluating damage of an organ tissue, in particular ischemic damage/injury of an organ tissue. The method includes the steps of measuring the concentration of at least one marker molecule in the perfusate of the organ tissue, where the measured concentration of the at least one marker molecule in the perfusate is used in at least one computer based prediction algorithm for generating at least one success score. The success score has been previously defined based on at least one parameter value of at least one pre-defined parameter. The at least one parameter value is determined after a transplantation of the organ tissue; and wherein based on the at least one success score at least one signal and/or at least one set of data is generated for facilitating the decision, if the organ tissue is suitable for transplantation or not.

Abstract: The present invention relates to a perfusion loop assembly for an ex vivo liver perfusion including: a pump for providing a fluid flow of a perfusion fluid through a first branch line and a second branch line; the first branch line being configured to provide a first portion of the perfusion fluid to the hepatic artery of the liver; the first branch line being coupled with a first gas exchanger, the second branch line being configured to provide a second portion of the perfusion fluid to the portal vein of the liver; the second branch line further including a first valve for controlling the flow of the perfusion fluid into the portal vein of the liver, a liver chamber assembly configured to hold the liver ex vivo, a liver outlet line attached to the vena cava of the ex vivo liver, at least one reservoir connected to the liver outlet and upstream from the pump.

Abstract: A method of detecting at least one gene modification such as a mutation in a gene includes carrying out an asymmetric polymerase chain reaction (PCR) with a combined use of at least one detectable mutation-specific hybridization probe (sensor probe) and at least one wild-type specific blocking agent which inhibits a binding of the at least one detectable mutation-specific hybridization probe (sensor probe) to a wild-type gene so as to provide at least one of a selective intensification and an amplification of a detection of a gene segment of a mutation gene having a gene modification.

Abstract: A method and a device to thermally fragment rock for excavation of vertical and directional boreholes in rock formations, preferentially hard rock, using highly exothermic reactions. Exothermic reactions are initiated directly in the pressurized, aqueous environment of a water-based drilling fluid preferably above the critical pressure of water (221 bar). After reaction onset temperatures within the reaction zone exceed the critical temperature for water (374° C.) providing supercritical conditions, which favor the stabilization of the reaction, e.g. a supercritical hydrothermal flame. Since reactions can be run directly in a water-based drilling fluid, the method proposed here allows high density drilling action as in conventional rotary drilling. A part from the hot reaction zone of the proposed reaction can be brought directly to the rock surface in case of hard polycrystalline rock, where high temperatures are required.

Abstract: A method for detecting at least one gene modification, such as a mutation in a gene, such as a gene that codes for a protein associated with at least one of a tumor and a cancer. The method includes providing a detectable hybridization probe (sensor probe) which interacts with/binds to a gene not having a gene modification (wild type gene) and with a gene having a gene modification (mutation gene). The detectable hybridization probe (sensor probe) has at least one of a higher specificity, a higher binding affinity and a higher selectivity for the gene not having a gene modification (wild type gene) compared to the gene having a gene modification (mutation gene). At least one gene modification is detected with the detectable hybridization probe (sensor probe).

Abstract: A method of detecting at least one gene modification such as a mutation in a gene includes carrying out an asymmetric polymerase chain reaction (PCR) with a combined use of at least one detectable mutation-specific hybridization probe (sensor probe) and at least one wild-type specific blocking agent which inhibits a binding of the at least one detectable mutation-specific hybridization probe (sensor probe) to a wild-type gene so as to provide at least one of a selective intensification and an amplification of a detection of a gene segment of a mutation gene having a gene modification.

Abstract: A method and a device to thermally fragment rock for excavation of vertical and directional boreholes in rock formations, preferentially hard rock, using highly exothermic reactions. Exothermic reactions are initiated directly in the pressurized, aqueous environment of a water-based drilling fluid preferably above the critical pressure of water (221 bar). After reaction onset temperatures within the reaction zone exceed the critical temperature for water (374° C.) providing supercritical conditions, which favor the stabilization of the reaction, e.g. a supercritical hydrothermal flame. Since reactions can be run directly in a water-based drilling fluid, the method proposed here allows high density drilling action as in conventional rotary drilling. A part from the hot reaction zone of the proposed reaction can be brought directly to the rock surface in case of hard polycrystalline rock, where high temperatures are required.

Abstract: The present invention relates to a method of treating non-small cell lung cancer with FLT-3 kinase inhibitor such as PKC412. The invention also relates to a pharmaceutical combination of a FLT-3 kinase inhibitor and an activator of permeabilization of the mitochondrial outer membrane, such as an activator of BAK. It also relates to the use of a pharmaceutical combination of an activator of permeabilization of the mitochondrial outer membrane and a FLT-3 kinase inhibitor for the treatment of non-small cell lung cancer and the use of such a pharmaceutical composition for the manufacture of a medicament for the treatment of same.

Abstract: The present invention relates to an implant (1) with at least partially a roughened surface (2), which is at least partially covered by an organic or polymeric intermediate layer (3) and attached thereto a top layer (4). The top layer (4) provides for a controlled release of at least one bioactive agent. A kit for preparing such an implant is also described.

Abstract: The present invention relates to an electrosurgical cutting device for the cutting and/or coagulation of tissue with RF current. A cutting electrode and a patient plate are connectable to the terminals of a RF generator. A control system, using a condition change arising at the cutting area during cutting as a controlled value, serves to control the RF current produced by the RF generator as a manipulated variable. The invention is characterized in that, for control of the RF-voltage of the RF generator, the control system responds to d-c voltage that arises at the cutting area during cutting with the RF current.