Patents by Inventor Martine Nguyen

Martine Nguyen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Yeast strains for the production of mature heterologous proteins, especially hirudin
    Patent number: 5879926
    Abstract: The present invention relates to an improved system for producing mature heterologous proteins, and especially hirudin, by means of yeasts comprising an expression vector containing a sequence coding for the heterologous protein. This improvement is characterized by amplification of the KEX2 gene of yeast, coding for the endoprotease yscF. The amplification is carried out either by integration of one or more copies of all or part of the KEX2 gene in the yeast genome, or by insertion of one or more copies of all or part of the KEX2 gene into the vector for expression of the heterologous protein.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: March 9, 1999
    Assignee: Transgene S.A.
    Inventors: Yves Lemoine, Martine Nguyen, Tilman Achstetter
  • Application of novel DNA fragments as a coding sequence for a signal peptide for the secretion of mature proteins by recombinant yeast, expression cassettes, transformed yeast and corresponding process for the preparation of proteins
    Patent number: 5631144
    Abstract: The invention relates to new DNA fragments and to their application as a DNA coding fragment for a signal peptide which can be used for the secretion of proteins, said peptide including a sequence of amino-acids which show a degree of correspondence of at least 60% with the sequence of amino-acids (I) or (II), preferably with the sequence (II). The sequences (I) and (II) are as follows: (I) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gl n. (II) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gl n-Val-Ser-Ala.
    Type: Grant
    Filed: February 7, 1995
    Date of Patent: May 20, 1997
    Assignee: Transgene S.A.
    Inventors: Yves Lemoine, Martine Nguyen, Tilman Achstetter, Jean-Marc Reichhart
  • Yeast vector coding for heterologous gene fusions linked via KEX2 cleavage site and coding for truncated KEX2 genes
    Patent number: 5521093
    Abstract: The present invention relates to an improved system for producing mature heterologous proteins, and especially hirudin, by means of yeasts comprising an expression vector containing a sequence coding for the heterologous protein. This improvement is characterized by amplification of the KEX2 gene of yeast, coding for the endoprotease yscF. The amplification is carried out either by integration of one or more copies of all or part of the KEX2 gene in the yeast genome, or by insertion of one or more copies of all or part of the KEX2 gene into the vector for expression of the heterologous protein.
    Type: Grant
    Filed: February 23, 1995
    Date of Patent: May 28, 1996
    Assignee: Transgene S.A.
    Inventors: Yves Lemoine, Martine Nguyen, Tilman Achstetter
  • Saccharomyces cerevisiae strain productive of a heterologous protein and process for preparing the said heterologous protein by fermentation of the said strain
    Patent number: 5162208
    Abstract: The invention relates to a new strain of Saccharomyces cerevisiae productive of a heterologous protein, and more especially of hirudin, characterized by a suppression of the proteolytic function encoded by the PRC1 gene. More specifically, this deficiency is due to a mutation or more especially to a deletion of the PRC1 structural gene.
    Type: Grant
    Filed: March 29, 1990
    Date of Patent: November 10, 1992
    Assignee: Transgene S.A.
    Inventors: Yves Lemoine, Martine Nguyen, Tilman Achstetter