Patents by Inventor Masae Iiboshi

Masae Iiboshi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for improving the thermostability of sucrose phosphorylase (SP)
    Patent number: 7968309
    Abstract: A sucrose phosphorylase (SP) having improved thermostability obtained by modifying a natural SP and a method for producing the SP having improved thermostability is provided. This SP having improved thermostability has an amino acid residue which is different from that of the natural sucrose phosphorylase, in at least one position selected from the group consisting of a position corresponding to position 14, a position corresponding to position 29 and a position corresponding to position 44 in motif sequence 1; a position corresponding to position 7, a position corresponding to position 19, a position corresponding to position 23 and a position corresponding to position 34 in motif sequence 2; and a position corresponding to position 19 in motif sequence 3, and wherein the enzyme activity of the SP having improved thermostability at 37° C., after heating the SP having improved thermostability in 20 mM Tris buffer (pH 7.0) at 55° C.
    Type: Grant
    Filed: August 31, 2004
    Date of Patent: June 28, 2011
    Assignee: Ezaki Glico Co., Ltd.
    Inventors: Kazutoshi Fujii, Masae Iiboshi, Michiyo Yanase, Hiroki Takata, Takeshi Takaha, Takashi Kuriki
  • Method for Improving the Thermostability of Sucrose Phosphorylase (Sp)
    Publication number: 20080206822
    Abstract: A sucrose phosphorylase (SP) having improved thermostability obtained by modifying a natural SP and a method for producing the SP having improved thermostability is provided. This SP having improved thermostability has an amino acid residue which is different from that of the natural sucrose phosphorylase, in at least one position selected from the group consisting of a position corresponding to position 14, a position corresponding to position 29 and a position corresponding to position 44 in motif sequence 1; a position corresponding to position 7, a position corresponding to position 19, a position corresponding to position 23 and a position corresponding to position 34 in motif sequence 2; and a position corresponding to position 19 in motif sequence 3, and wherein the enzyme activity of the SP having improved thermostability at 37° C., after heating the SP having improved thermostability in 20 mM Tris buffer (pH 7.0) at 55° C.
    Type: Application
    Filed: August 31, 2004
    Publication date: August 28, 2008
    Inventors: Kazutoshi Fujii, Masae Iiboshi, Michiyo Yanase, Hiroki Takata, Takeshi Takaha, Takashi Kuriki