Abstract: A system that easily and stably separates various living cells of interest from a living body-derived tissue, the system including: a suspension unit that prepares a suspension by adding a proteolytic enzyme solution to a living body-derived tissue based on a parameter and shaking the tissue to which the solution has been added; a measurement unit that acquires information regarding the suspension; and an analysis unit that specifies a position of living cells from the information acquired by the measurement unit. Methods for separating various living cells of interest from a living body-derived tissue are also disclosed.

Abstract: A system that non-destructively and quickly determines a degree of senescence of cells to be cultured by utilizing the discovery that when cells are adhered to a surface of a culture substrate in a culture medium, the degree of cytoplasm spreading on the culture substrate is indicative of the degree of senescence. The degree of the spreading can be detected based on a result of brightness, reflectance, or image processing, and the degree of senescence is determined. It is also possible to determine the degree of senescence from a rate of the spread.

Abstract: A system that easily and stably separates various living cells from a living body-derived tissue, the system including: a mincing unit that minces the living body-derived tissue based on a parameter; a measurement unit that acquires information regarding the living body-derived tissue being minced; and an analysis unit that calculates a ratio of impurities to the living body-derived tissue being minced from the information acquired by the measurement unit. Methods for separating various living cells from a living body-derived tissue are also disclosed.

Abstract: A bioadhesive sheet-shaped material configured to be attached onto a surface of an organ, a method for producing the bioadhesive sheet-shaped material, and a method for treating a disease by using the bioadhesive sheet-shaped material. The bioadhesive sheet-shaped material includes an extracellular matrix layer, a sheet-shaped cell culture, and a biodegradable gel layer, where the sheet-shaped cell culture is interposed between the extracellular matrix layer and the biodegradable gel layer, and the bioadhesive sheet-shaped material is by attaching the extracellular matrix layer onto a surface of an organ.

Abstract: Systems and methods of the present disclosure include a cell culture, a method for producing the cell culture, a kit for producing the cell culture, a method for treating inflammatory disease using the cell culture, and the like, for treating inflammatory disease. The cell culture containing cells can be derived from skeletal muscle.

Abstract: Disclosed are embodiments of a sheet-shaped cell culture for promoting healing of a lumen organ having a damaged part, particularly tissue of a duodenum, a method for producing a sheet-shaped cell culture. Also disclosed are embodiments of a method for regenerating a damaged part in a lumen organ using a sheet-shaped cell culture described herein.

Abstract: A container for containing a freezing bag filled with biological tissue and for cooling and warming the freezing bag. The container includes a main body possessing an inner surface, a first side surface and a second side surface positioned opposite the first side surface. The main body is substantially rectangular parallelepiped shaped. The container includes at least one opening in at least one of the first side surface and the second side surface of the container, and at least two ridges spaced apart from one another on the inner surface of the container to create an air gap between the spaced apart ridges, the inner surface of the container and the outer surface of the freezing bag.

Abstract: A container for containing a freezing bag filled with biological tissue and for cooling and warming the freezing bag. The container includes a main body possessing an inner surface, a first side surface and a second side surface positioned opposite the first side surface. The main body is substantially rectangular parallelepiped shaped. The container includes at least one opening in at least one of the first side surface and the second side surface of the container, and at least two ridges spaced apart from one another on the inner surface of the container to create an air gap between the spaced apart ridges, the inner surface of the container and the outer surface of the freezing bag.