Patents by Inventor Masako SUDA
Masako SUDA has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11459574Abstract: Provided is a method for improving productivity in producing an organic compound in a bacterium that originally does not have an inherent ED pathway. In one aspect, provided is a transformant of a coryneform bacterium that is obtained by introducing the Entner-Doudoroff pathway into the coryneform bacterium as a host. In another aspect, provided is a transformant of a coryneform bacterium that is obtained by introducing, into a coryneform bacterium as a host a gene in which an enzyme having glucose-6-phosphate dehydrogenase activity is encoded, a gene in which an enzyme having 6-phosphogluconate dehydratase activity is encoded, and a gene in which an enzyme having 2-keto-3-deoxy-6-phosphogluconate aldolase activity is encoded.Type: GrantFiled: March 27, 2019Date of Patent: October 4, 2022Assignee: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTHInventors: Masayuki Inui, Masako Suda, Naoto Kato, Satoshi Hasegawa, Takahisa Kogure, Toru Jojima
-
Patent number: 11359217Abstract: Provided is a transformant of a microorganism that has improved catechol productivity.Type: GrantFiled: February 18, 2019Date of Patent: June 14, 2022Assignee: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTHInventors: Masayuki Inui, Kazumi Hiraga, Masako Suda, Takeshi Kubota
-
Publication number: 20220025412Abstract: Provided is a microorganism that is able to produce 2-phenylethanol at a high concentration, and a method of efficiently producing 2-phenylethanol by using a saccharide as a raw material. Provided is a coryneform bacterium transformant in which a shikimate pathway is activated, and further, a gene that encodes an enzyme having phenylpyruvate decarboxylase activity is introduced in such a manner that the gene can be expressed. Also provided is a 2-phenylethanol producing method that includes causing the coryneform bacterium transformant according to the present disclosure to react in water containing a saccharide.Type: ApplicationFiled: December 19, 2019Publication date: January 27, 2022Applicants: Research Institute of Innovative Technology for the Earth, Sumitomo Bakelite Co., Ltd.Inventors: Masayuki Inui, Kazumi Hiraga, Masako Suda, Masayoshi Hashizume, Junya Ishida
-
Publication number: 20210222211Abstract: Provided is a transformant of a microorganism that has improved catechol productivity.Type: ApplicationFiled: February 18, 2019Publication date: July 22, 2021Applicant: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTHInventors: Masayuki INUI, Kazumi HIRAGA, Masako SUDA, Takeshi KUBOTA
-
Patent number: 10988743Abstract: Provided is a microorganism that is capable of efficiently producing para-aminobenzoic acid (4-ABA) or a salt thereof, using saccharides as raw materials, and a method for efficiently producing 4-ABA or a salt thereof by using this microorganism. A transformant obtained by introducing, into a coryneform bacterium, a gene that encodes 4-amino-4-deoxychorismate lyase, a gene that encodes a para-aminobenzoate synthase component I, and a gene that encodes a para-aminobenzoate synthase component II, is capable of efficiently producing 4-ABA or a salt thereof from saccharides.Type: GrantFiled: February 24, 2017Date of Patent: April 27, 2021Assignees: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH, SUMITOMO BAKELITE CO., LTD.Inventors: Masayuki Inui, Kazumi Hiraga, Masako Suda, Takeshi Kubota
-
Patent number: 10961526Abstract: Provided is a microorganism that is able to efficiently produce protocatechuic acid or a salt thereof by using a saccharide as a raw material, and a method of efficiently producing protocatechuic acid or a salt thereof by using the microorganism. Provided is a transformant having protocatechuic acid producing ability, subjected to modifications of enhancement of 3-dehydroshikimate dehydratase activity; enhancement of chorismate pyruvate lyase activity; and enhancement of 4-hydroxybenzoate hydroxylase activity. Also provided is a method of producing protocatechuic acid or a salt thereof, including the step of culturing the transformant in a reaction solution containing a saccharide so as to cause the transformant to produce protocatechuic acid or a salt thereof.Type: GrantFiled: February 24, 2017Date of Patent: March 30, 2021Assignees: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH, SUMITOMO BAKELITE CO., LTD.Inventors: Masayuki Inui, Kazumi Hiraga, Masako Suda, Takahisa Kogure
-
Publication number: 20210054386Abstract: Provided is a method for improving productivity in producing an organic compound in a bacterium that originally does not have an inherent ED pathway. In one aspect, provided is a transformant of a coryneform bacterium that is obtained by introducing the Entner-Doudoroff pathway into the coryneform bacterium as a host. In another aspect, provided is a transformant of a coryneform bacterium that is obtained by introducing, into a coryneform bacterium as a host a gene in which an enzyme having glucose-6-phosphate dehydrogenase activity is encoded, a gene in which an enzyme having 6-phosphogluconate dehydratase activity is encoded, and a gene in which an enzyme having 2-keto-3-deoxy-6-phosphogluconate aldolase activity is encoded.Type: ApplicationFiled: March 27, 2019Publication date: February 25, 2021Applicant: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTHInventors: Masayuki INUI, Masako SUDA, Naoto KATO, Satoshi HASEGAWA, Takahisa KOGURE, Toru JOJIMA
-
Patent number: 10738296Abstract: The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency. (A) A mutant chorismate-pyruvate lyase obtained by replacing the valine at position 80 in a chorismate-pyruvate lyase (ubiC) from Pantoea ananatis consisting of the amino acid sequence of SEQ ID NO: 1 with one or more other amino acids. (B) A mutant chorismate-pyruvate lyase obtained by replacing an amino acid in another chorismate-pyruvate lyase, the amino acid being at a position enzymologically homologous with that of the above valine, with one or mere other amino acids.Type: GrantFiled: January 10, 2019Date of Patent: August 11, 2020Assignee: GREEN CHEMICALS CO., LTD.Inventors: Masayuki Inui, Kazumi Hiraga, Masako Suda, Ryoma Hashimoto
-
Publication number: 20190194629Abstract: Provided is a microorganism that is capable of efficiently producing para-aminobenzoic acid (4-ABA) or a salt thereof, using saccharides as raw materials, and a method for efficiently producing 4-ABA or a salt thereof by using this microorganism. A transformant obtained by introducing, into a coryneform bacterium, a gene that encodes 4-amino-4-deoxychorismate lyase, a gene that encodes a para-aminobenzoate synthase component I, and a gene that encodes a para-aminobenzoate synthase component II, is capable of efficiently producing 4-ABA or a salt thereof from saccharides.Type: ApplicationFiled: February 24, 2017Publication date: June 27, 2019Applicants: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH, SUMITOMO BAKELITE CO., LTD.Inventors: Masayuki INUI, Kazumi HIRAGA, Masako SUDA, Takeshi KUBOTA
-
Publication number: 20190136222Abstract: The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency. (A) A mutant chorismate-pyruvate lyase obtained by replacing the valine at position 80 in a chorismate-pyruvate lyase (ubiC) from Pantoea ananatis consisting of the amino acid sequence of SEQ ID NO: 1 with one or more other amino acids. (B) A mutant chorismate-pyruvate lyase obtained by replacing an amino acid in another chorismate-pyruvate lyase, the amino acid being at a position enzymologically homologous with that of the above valine, with one or mere other amino acids.Type: ApplicationFiled: January 10, 2019Publication date: May 9, 2019Applicant: GREEN CHEMICALS CO., LTD.Inventors: Masayuki INUI, Kazumi HIRAGA, Masako SUDA, Ryoma HASHIMOTO
-
Publication number: 20190119664Abstract: Provided is a microorganism that is able to efficiently produce protocatechuic acid or a salt thereof by using a saccharide as a raw material, and a method of efficiently producing protocatechuic acid or a salt thereof by using the microorganism. Provided is a transformant having protocatechuic acid producing ability, subjected to modifications (A), (B), and (C) below: (A) enhancement of 3-dehydroshikimate dehydratase activity; (B) enhancement of chorismate pyruvate lyase activity; and (C) enhancement of 4-hydroxybenzoate hydroxylase activity. Also provided is a method of producing protocatechuic acid or a salt thereof, including the step of culturing the transformant in a reaction solution containing a saccharide so as to cause the transformant to produce protocatechuic acid or a salt thereof.Type: ApplicationFiled: February 24, 2017Publication date: April 25, 2019Applicants: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTH, SUMITOMO BAKELITE CO., LTD.Inventors: Masayuki INUI, Kazumi HIRAGA, Masako SUDA, Takahisa KOGURE
-
Patent number: 10221409Abstract: The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency. (A) A mutant chorismate-pyruvate lyase obtained by replacing the valine at position 80 in a chorismate-pyruvate lyase (ubiC) from Pantoea ananatis consisting of the amino acid sequence of SEQ ID NO: 1 with one or more other amino acids. (B) A mutant chorismate-pyruvate lyase obtained by replacing an amino acid in another chorismate-pyruvate lyase, the amino acid being at a position enzymologically homologous with that of the above valine, with one or more other amino acids.Type: GrantFiled: May 13, 2015Date of Patent: March 5, 2019Assignee: GREEN CHEMICALS CO., LTD.Inventors: Hideaki Yukawa, Masayuki Inui, Kazumi Hiraga, Masako Suda, Ryoma Hashimoto
-
Patent number: 10208313Abstract: A coryneform bacterium transformant engineered by the following (A) to (D): (A) enhancement of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase activity; (B) prevention, inhibition, or reduction of intracellular sugar uptake mediated by phosphotransferase system (PTS); (C) enhancement of intracellular sugar uptake activity mediated by a sugar transporter different from phosphotransferase system and enhancement of glucokinase activity; and (D) enhancement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity is capable of efficiently producing shikimic acid or the like from a sugar.Type: GrantFiled: August 20, 2015Date of Patent: February 19, 2019Assignee: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTHInventors: Masayuki Inui, Masako Suda, Kazumi Hiraga, Takahisa Kogure
-
Patent number: 10017791Abstract: A transformant constructed by introducing a gene which encodes an enzyme having chorismate-pyruvate lyase activity into a coryneform bacterium as a host is capable of efficiently producing 4-hydroxybenzoic acid or a salt thereof from a sugar. When the transformant is cultured under aerobic conditions where the transformant does not grow, 4-hydroxybenzoic acid or a salt thereof can be produced in a particularly efficient manner.Type: GrantFiled: April 7, 2015Date of Patent: July 10, 2018Assignee: GREEN CHEMICALS CO., LTD.Inventors: Hideaki Yukawa, Masayuki Inui, Kazumi Hiraga, Masako Suda, Ryoma Hashimoto
-
Publication number: 20180044688Abstract: A coryneform bacterium transformant engineered by the following (A) to (D): (A) enhancement of 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase activity; (B) prevention, inhibition, or reduction of intracellular sugar uptake mediated by phosphotransferase system (PTS); (C) enhancement of intracellular sugar uptake activity mediated by a sugar transporter different from phosphotransferase system and enhancement of glucokinase activity; and (D) enhancement of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity is capable of efficiently producing shikimic acid or the like from a sugar.Type: ApplicationFiled: August 20, 2015Publication date: February 15, 2018Applicant: RESEARCH INSTITUTE OF INNOVATIVE TECHNOLOGY FOR THE EARTHInventors: Masayuki INUI, Masako SUDA, Kazumi HIRAGA, Takahisa KOGURE
-
Publication number: 20170114374Abstract: A transformant constructed by introducing a gene which encodes an enzyme having chorismate-pyruvate lyase activity into a coryneform bacterium as a host is capable of efficiently producing 4-hydroxybenzoic acid or a salt thereof from a sugar. When the transformant is cultured under aerobic conditions where the transformant does not grow, 4-hydroxybenzoic acid or a salt thereof can be produced in a particularly efficient manner.Type: ApplicationFiled: April 7, 2015Publication date: April 27, 2017Applicant: GREEN PHENOL DEVELOPMENT CO., LTD.Inventors: Hideaki YUKAWA, Masayuki INUI, Kazumi HIRAGA, Masako SUDA, Ryoma HASHIMOTO
-
Publication number: 20170073658Abstract: The mutant chorismate-pyruvate lyase (A) or (B) as described below is capable of producing 4-hydroxybenzoic acid or a salt thereof with sufficient practical efficiency. (A) A mutant chorismate-pyruvate lyase obtained by replacing the valine at position 80 in a chorismate-pyruvate lyase (ubiC) from Pantoea ananatis consisting of the amino acid sequence of SEQ ID NO: 1 with one or more other amino acids. (B) A mutant chorismate-pyruvate lyase obtained by replacing an amino acid in another chorismate-pyruvate lyase, the amino acid being at a position enzymologically homologous with that of the above valine, with one or more other amino acids.Type: ApplicationFiled: May 13, 2015Publication date: March 16, 2017Applicant: GREEN PHENOL DEVELOPMENT CO., LTD.Inventors: Hideaki YUKAWA, Masayuki INUI, Kazumi HIRAGA, Masako SUDA, Ryoma HASHIMOTO