Abstract: It is an object of the present invention to evaluate the induction of a drug-metabolizing enzyme by a test substance more accurately than ever before, by simple operations, using a vector in which a reporter gene is connected downstream of the expression control region of a drug-metabolizing enzyme gene and a cell into which the aforementioned vector is introduced. The present invention provides a vector for producing a cell that is used for evaluation of the induction of a drug-metabolizing enzyme by a test substance and the cytotoxicity of the test substance, the vector comprising an expression control region of a fetus-specific gene, a first reporter gene, an expression control region of a drug-metabolizing enzyme gene, and a second reporter gene, wherein the first reporter gene is located downstream of the expression control region of the fetus-specific gene and the second reporter gene is located downstream of the expression control region of the drug-metabolizing enzyme gene.

Abstract: It is intended to provide a regenerable cell in which a desired chromosome has been deleted, a method for producing the cell, and a gene cassette and a kit to be used for the method. More particularly, it is intended to obtain an individual corresponding pluripotent stem cell easily and simply. More specifically, it is intended to efficiently establish a cell, tissue or organ that can be a donor for treating a disease without causing immune rejection response, without newly obtaining and establishing a stem cell such as ES cell from the individual, and without using an ovum as a material. It was achieved by a gene cassette in which not two recombinase recognition sites in a cis orientation are inserted, but one recombinase recognition site or two recombinase recognition sites in an opposite orientation is/are inserted and a marker gene is connected, and by application of the gene cassette to a cell fusion technique.

Abstract: An object of the present invention is to efficiently establish cells, tissues, and organs capable of serving as donors for treating diseases, without eliciting immune rejection reactions, without starting with an egg cell. This object was achieved by providing a pluripotent stem cell having a desired genome. The cell was produced by treating with a reprogramming agent, producing a fusion cell of an MHC deficient stem cell with a somatic cell, or after producing a fusion cell of a stem cell with a somatic cell, removing a gene derived from the stem cell by performing genetic manipulation with a retrovirus.

Abstract: A gene is provided, which can be used as a marker for determining whether a certain cell, particularly an undifferentiated cell including a tissue stem cell, has pluripotency or an undifferentiated state. The gene is called Stm and includes a Stm1 gene, which is expressed specifically in a cell under an undifferentiated state if the cell has pluripotency. A kit for determining a differentiated state of a cell is also provided. The kit comprises (a) an agent capable of reacting specifically with a Stm gene or a Stm gene product; and (b) means for determining whether or not the Stm gene is expressed in the cell.

Abstract: By exposing somatic cells to a component derived from ES cells to act on somatic cells and detecting the activity thereof, the reprogramming agent can be screened. Further, by exposing somatic cells to a component including a reprogramming agent or an isolated reprogramming agent, the somatic cells can be reprogrammed. Furthermore, according to the present invention, since the tetraploid cells have proliferating capability and pluripotency, such cells can be differentiated and the cells, tissues or organs, which can be used for transplantation, can be produced.

Abstract: An object of the present invention is to efficiently establish cells, tissues, and organs capable of serving as donors for treating diseases, without eliciting immune rejection reactions, without starting with an egg cell. This object was achieved by providing a pluripotent stem cell having a desired genome. The cell was produced by treating with a reprogramming agent, producing a fusion cell of an MHC deficient stem cell with a somatic cell, or after producing a fusion cell of a stem cell with a somatic cell, removing a gene derived from the stem cell by performing genetic manipulation with a retrovirus.

Abstract: A water and oil repellent composition comprising a compound of the following formula (1) and a thermoplastic polymer, as essential components: where the symbols in the formula (1) have the following meanings: Rf: a C1-20 polyfluoroalkyl group, X: (CH2)m, wherein m is an integer of from 1 to 10, A, A1, A2: each independently, O, S or N(Z3), wherein Z3 is a hydrogen atom or an alkyl group, Z1: an octadecyl group, Z2: an alkyl group, or an alkyl group having at least one hydrogen atom substituted, W: a trivalent organic group, and a, b: each independently, 1 or 2, provided that a+b is 2 or 3.

Abstract: A water and oil repellent composition comprising a compound of the following formula (1) and a thermoplastic polymer, as essential components: 1

Abstract: A reactive hot melt adhesive containing, as the main component, a blocked prepolymer made by reacting a linear prepolymer having isocyanate groups with a blocking agent, said blocked prepolymer having a number average molecular weight of at least 11,000 and a melt viscosity of at least 1,000 poise at a temperature of 110.degree. C.