Abstract: A method for the extraction and purification of a plasmid DNA which comprises (1) collecting a culture medium containing a transformant into a first cartridge for the extraction and purification of the DNA, (2) subjecting said transformant to bacteriolysis and RNA degradation conditions, (3) recovering an extract containing solubilized DNA from said first cartridge, (4) transferring said recovered extract to a second cartridge for the extraction and purification of the DNA, and (5) absorbing, washing and eluting purified plasmid DNA from said second cartridge; and a device for the extraction and purification of a DNA which comprises a first cartridge for the extraction and purification of the DNA having at least a trap filter and a membrane filter, and a second cartridge for the extraction and purification of the DNA having at least a glass fiber filter, a glass powder layer and a membrane filter.

Abstract: An object of the present invention is to carry out the extraction and purification of DNA in a short period of time. This object is accomplished by DNA extraction and purification apparatus illustrated in the drawing. This apparatus includes a pipetting unit which can be horizontally moved along a rail by means of a transfer device. This apparatus also includes tube racks which can be moved horizontally and vertically. In these tube racks are disposed filter tubes (not shown) for the extraction and purification of DNA. Moreover, a waste liquid vat and a recovery vat are disposed below these tube racks. The waste liquid vat and the recovery vat are each provided with a suction port (not shown) to be connected to a vacuum pump.

Abstract: An object of the present invention is to carry out the extraction and purification of DNA in a short period of time. This object is accomplished by DNA extraction and purification apparatus illustrated in the drawing. This apparatus includes a pipetting unit which can be horizontally moved along a rail by means of a transfer device. This apparatus also includes tube racks which can be moved horizontally and vertically. In these tube racks are disposed filter tubes (not shown) for the extraction and purification of DNA. Moreover, a waste liquid vat and a recovery vat are disposed below these tube racks. The waste liquid vat and the recovery vat are each provided with a suction port (not shown) to be connected to a vacuum pump.