Abstract: An object of the present invention relates to detecting a target substance with high contrast. The invention relates to analysis of a target substance using a light-transmitting substrate and a metal for inducing plasmon resonance, and further using a low refractive index layer with an opening portion, which forms an interface with the substrate, and which has a lower refractive index than the substrate. Light emitted from a substrate side is totally reflected at the interface to irradiate the metal arranged in the opening portion with evanescent light. Light generated from the target substance by plasmon resonance of the evanescent light is detected. According to the invention, the radiation of evanescent light to a martial other than the target substance can be reduced, and thereby light emission from the martial other than the target substance, e.g., a molecule floating around the target substance, can be reduced.

Abstract: The present invention relates to a nucleic acid analysis device for analysis of nucleic acid in a sample through fluorometry, in which a localized surface plasmon by light irradiation, and in which a nucleic acid probe or a nucleic acid synthase for the analysis of the nucleic acid in the sample is disposed in a region of generation of the surface plasmon. The present invention allows the fluorescence intensifying effect of the surface plasmon to be produced efficiently and also enables the immobilization of a DNA probe or the nucleic acid synthase in a region on which the fluorescence intensifying effect is exerted, thus making it possible to carry out a measurement on the base elongation reaction without having to remove the unreacted substrate with the fluorescent molecule.

Abstract: The present invention relates to a method for detecting fusion gene transcripts resulting from chromosomal translocation. Specifically, the method of the present invention comprises allowing at least two or more probes, each of which contains a partial base sequence of exons which sandwich the breakpoint of a fusion gene or complementary base sequence thereof, and each of which immobilized on a support, to hybridize with a sample containing a nucleic acid derived from a fusion gene, thereby allowing the detection of two or more fusion genes at a time.

Abstract: This invention relates to a method of simply and accurately diagnosing depression by assaying the expression levels of a specific group of genes in a subject's peripheral leukocytes. In this method, the expression level of at least one gene selected from among apoptosis-associated genes, ATPase-associated genes, cell cycle-associated genes, cytokine-associated genes, heat shock protein-associated genes, polymerase-associated genes, GTP-binding protein-associated genes, protein kinase C-associated genes, and mitochondrial cytochrome C oxidase-associated genes is analyzed using mRNA of a subject's peripheral blood, and symptoms of depression in the subject are diagnosed based on the results of such analysis.

Abstract: The present invention provides nucleic acid arrays with improved sensitivity for a nucleic acid. The array comprises various kinds of nucleic acid probes, which are capable of hybridizing to the nucleic acid, immobilized at different positions on a substrate. Single-stranded nucleic acid probes are immobilized on the substrate by covalent bond, and functional groups that can have negative charge by dissociating in an aqueous solution or by hydrolysis are introduced on the surface of regions of the substrate on which no nucleic acid probe is immobilized.

Abstract: The efficacy of an interferon &bgr; treatment is evaluated by using a database on correlation between the efficacy of the interferon &bgr; treatment and the gene expression levels of at least one interferon induced protein gene, at least one interferon regulation factor gene, and at least one chemokine gene, and determining the expression levels of the genes in messenger RNAs derived from leukocytes in the peripheral blood of a subject.

Abstract: Provided are a method and a system for allergy analysis by using RNA derived from leukocytes extracted from peripheral blood as such, correcting the observed value, and comparing the balance of helper T (Th) cells (Th1/Th2) with the number of Th1 cells/the number of Th2 cells in a nucleic acid sample or the Th1/Th2 data of a patient and of a normal volunteer. Also provided are a method and a system for allergy analysis using a highly reproducible and reliable array having a minimal number of DNA fragments (oligonucleotides) thereon, which is realized by identifying essential gene groups for the Th1/Th2 assay. According to the present invention, it is possible to analyze the existence of a specific cell in a sample containing several types of cells without isolating the cell of interest.

Abstract: It is needed to implement probing sequences having no potentiality of cross-hybridization, equal Tms, and less secondary structures for the probing sequences for DNA arrays so that the sensitivity and repeatability of measurement may be ensured. The probing sequences for the DNA arrays can be determined by following at least two steps, one being a step for creating a list of candidate probes for the DNA probe arrays, which are made of partial sequences of genome or cDNA sequences (the list-up step) and the other being a step for discarding the candidate probes, of which melting temperatures deviate from a given range, which have higher stability of secondary structures, or which have the potentiality of cross-hybridization, from the primary candidate probe list (the filtering step). The present invention thus provides a method for designing probing DNA sequences to be fixed on the DNA probe arrays.

Abstract: The present invention provides nucleic acid arrays with improved sensitivity for a nucleic acid. The array comprises various kinds of nucleic acid probes, which are capable of hybridizing to the nucleic acid, immobilized at different positions on a substrate. Single-stranded nucleic acid probes are immobilized on the substrate by covalent bond, and functional groups that can have negative charge by dissociating in an aqueous solution or by hydrolysis are introduced on the surface of regions of the substrate on which no nucleic acid probe is immobilized.

Abstract: The present invention provides a photosensitive resin composition capable of forming an insulating film, which is superior in both a roughening property and an adhesiveness, and a via-hole, which is highly reliable in connection, and a multilayer printed circuit board. The present invention provides a photosensitive resin composition containing a first resin, which is an epoxy resin, and a second resin having a N-substituted carbamic acid ester atomic group and a radical polymeric unsaturated bond in its side chain. The second resin is desirably an oligomer having a repeating unit expressed by the following general formula (chem. 1) or (chem. 3) by 3-10 units. Where, X is H or CH3, Y and Z is H or an alkyl group of carbon number 1-4, n is 0 or 1, a part of R1 is an atomic group expressed by the following general formula (chem. 2), the residual R1 is a hydroxyl group, and R2 is an alkylene group of carbon number 1-4.