Patents by Inventor Masatoshi Yanagida
Masatoshi Yanagida has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240118272Abstract: Disclosed is a method for measuring an activity of alkaline phosphatase contained in an extracellular vesicle in a sample, including acquiring an activity value of alkaline phosphatase contained in an extracellular vesicle in a sample using a calibrator, in which the calibrator contains a conjugate in which a polypeptide having alkaline phosphatase activity and a polypeptide including an amino acid sequence of a membrane protein present on a surface of the extracellular vesicle are covalently bound.Type: ApplicationFiled: September 18, 2023Publication date: April 11, 2024Applicant: SYSMEX CORPORATIONInventors: Eiya TAMADA, Masatoshi Yanagida, Kazuto Yamashita, Elnaz Nakhael, Mami Onishi, Maria Kiriyama
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Patent number: 11808761Abstract: Disclosed is a method for measuring an immunostimulatory response of an immune cell, including (i) bringing a measurement target immune cell into contact with an immunostimulator, (ii) forming a contact surface with a substance different from the measurement target immune cell on the measurement target immune cell, (iii) bringing the measurement target immune cell into contact with a capturing body that binds to a surface antigen on the contact surface and is capable of generating an optical signal, (iv) detecting the optical signal generated from the capturing body, and (v) determining whether or not the measurement target immune cell from which the contact surface has been eliminated before detecting the optical signal has an immunostimulatory response, based on the detected optical signal.Type: GrantFiled: July 29, 2021Date of Patent: November 7, 2023Assignee: SYSMEX CORPORATIONInventors: Masatoshi Yanagida, Keiko Miwa, Yuma Oka
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Publication number: 20210356454Abstract: Disclosed is a method for measuring an immunostimulatory response of an immune cell, including (i) bringing a measurement target immune cell into contact with an immunostimulator, (ii) forming a contact surface with a substance different from the measurement target immune cell on the measurement target immune cell, (iii) bringing the measurement target immune cell into contact with a capturing body that binds to a surface antigen on the contact surface and is capable of generating an optical signal, (iv) detecting the optical signal generated from the capturing body, and (v) determining whether or not the measurement target immune cell from which the contact surface has been eliminated before detecting the optical signal has an immunostimulatory response, based on the detected optical signal.Type: ApplicationFiled: July 29, 2021Publication date: November 18, 2021Applicant: SYSMEX CORPORATIONInventors: Masatoshi YANAGIDA, Keiko MIWA, Yuma OKA
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Patent number: 11125740Abstract: Disclosed is a method for measuring an immunostimulatory response of an immune cell, including (i) bringing a measurement target immune cell into contact with an immunostimulator, (ii) forming a contact surface with a substance different from the measurement target immune cell on the measurement target immune cell, (iii) bringing the measurement target immune cell into contact with a capturing body that binds to a surface antigen on the contact surface and is capable of generating an optical signal, (iv) detecting the optical signal generated from the capturing body, and (v) determining whether or not the measurement target immune cell from which the contact surface has been eliminated before detecting the optical signal has an immunostimulatory response, based on the detected optical signal.Type: GrantFiled: April 10, 2018Date of Patent: September 21, 2021Assignee: SYSMEX CORPORATIONInventors: Masatoshi Yanagida, Keiko Miwa, Yuma Oka
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Publication number: 20210270812Abstract: Disclosed is a method for analyzing immune cells, comprising the steps of mixing whole blood containing immune cells with an immunostimulatory factor in vitro and labeling target molecules of the immune cells, and a step of acquiring information on localization of the target molecules on the immune cells by detecting the label.Type: ApplicationFiled: May 18, 2021Publication date: September 2, 2021Applicant: SYSMEX CORPORATIONInventors: Yuma OKA, Masatoshi YANAGIDA, Keiko MIWA
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Cell selection method, cell detection method, cell selection apparatus, and cell detection apparatus
Patent number: 11060134Abstract: Disclosed is a cell selection method including a sample preparation step of preparing a sample by performing staining of nucleic acid in each of cells by a first fluorescent dye; and hybridization with respect to an evaluation target region in DNA in each cell by an evaluation probe including a second fluorescent dye; a light receiving step of applying light to the sample and receiving fluorescence from the first fluorescent dye and fluorescence from the second fluorescent dye; and a selection step of selecting an analysis target cell on the basis of intensity of the fluorescence from the first fluorescent dye and intensity of the fluorescence from the second fluorescent dye, wherein the first fluorescent dye is a dye that emits fluorescence having a first wavelength, and the second fluorescent dye is a dye that emits fluorescence having a second wavelength different from the first wavelength.Type: GrantFiled: November 29, 2016Date of Patent: July 13, 2021Assignee: SYSMEX CORPORATIONInventors: Yusuke Takahashi, Masatoshi Yanagida -
Patent number: 10801007Abstract: A cell detection device and a cell detection method is provided with which it is possible to efficiently acquire images of cells to be measured. Cell detection device comprises flow cell through which a measurement specimen that contains particles is caused to flow, particle detector for detecting the particles in the measurement specimen supplied to flow cell, particle sorter for sorting particles that satisfy a detection condition and other particles on the basis of the result of detection performed by particle detector, specimen supply part for supplying, to flow cell, an image-capture specimen that includes detection-condition-satisfying particles that have been sorted by particle sorter, and particle-image-capture part for capturing images of the particles in the sorted image-capture specimen supplied to flow cell.Type: GrantFiled: October 3, 2019Date of Patent: October 13, 2020Assignee: SYSMEX CORPORATIONInventors: Seiichiro Tabata, Masatoshi Yanagida
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Publication number: 20200264181Abstract: The present invention provides a method for determining a success or failure of a measurement of a target particle contained in a measurement sample. The method includes: detecting the target particle and other particle other than the target particle in the measurement sample; and determining the success or failure of the measurement of the target particle based on at least a detection result of the other particle.Type: ApplicationFiled: February 18, 2020Publication date: August 20, 2020Applicant: SYSMEX CORPORATIONInventors: Yusuke TAKAHASHI, Masatoshi YANAGIDA, Kentaro SHIRAI, Yuichi IJIRI, Shigeki IWANAGA
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Publication number: 20200173980Abstract: A method of determining a quality of a cell separation, according to an aspect, includes: separating, by running a specimen containing a first particle and a second particle in a flow path, the specimen to a first specimen containing the first particle and a second specimen containing the second particle, based on a difference in magnitude of a force exerted on each particle contained in the specimen; measuring first information on an amount of the first specimen and second information on an amount of the second specimen; and determining a quality of the separating based on the first information and the second information.Type: ApplicationFiled: November 27, 2019Publication date: June 4, 2020Applicant: SYSMEX CORPORATIONInventors: Yusuke TAKAHASHI, Kentaro SHIRAI, Masatoshi YANAGIDA, Shigeki IWANAGA, Yuichi IJIRI, Keiko YOSHIKAWA
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Publication number: 20200032196Abstract: A cell detection device and a cell detection method is provided with which it is possible to efficiently acquire images of cells to be measured. Cell detection device comprises flow cell through which a measurement specimen that contains particles is caused to flow, particle detector for detecting the particles in the measurement specimen supplied to flow cell, particle sorter for sorting particles that satisfy a detection condition and other particles on the basis of the result of detection performed by particle detector, specimen supply part for supplying, to flow cell, an image-capture specimen that includes detection-condition-satisfying particles that have been sorted by particle sorter, and particle-image-capture part for capturing images of the particles in the sorted image-capture specimen supplied to flow cell.Type: ApplicationFiled: October 3, 2019Publication date: January 30, 2020Applicant: SYSMEX CORPORATIONInventors: Seiichiro TABATA, Masatoshi YANAGIDA
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Patent number: 10513679Abstract: A cell detection device and a cell detection method is provided with which it is possible to efficiently acquire images of cells to be measured. Cell detection device comprises flow cell through which a measurement specimen that contains particles is caused to flow, particle detector for detecting the particles in the measurement specimen supplied to flow cell, particle sorter for sorting particles that satisfy a detection condition and other particles on the basis of the result of detection performed by particle detector, specimen supply part for supplying, to flow cell, an image-capture specimen that includes detection-condition-satisfying particles that have been sorted by particle sorter, and particle-image-capture part for capturing images of the particles in the sorted image-capture specimen supplied to flow cell.Type: GrantFiled: May 19, 2016Date of Patent: December 24, 2019Assignee: SYSMEX CORPORATIONInventors: Seiichiro Tabata, Masatoshi Yanagida
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Publication number: 20190128793Abstract: A cell detection method and a cell detection system capable of performing target cell detection satisfactorily without interference and reducing the time required for detection of target cells are provided. The cell detection method includes a separation step of step S1 to obtain a sample containing target cells by removing at least a part of the non-target cells from a biological sample including target cells and non-target cells based on a difference in forces acting on target cells and non-target cells; a concentration step of step S3 of obtaining a measurement sample having a target cell concentration increased from the sample containing target cells; a detection of step S4 of detecting target cells by subjecting the measurement sample to an imaging flow cytometer.Type: ApplicationFiled: November 1, 2018Publication date: May 2, 2019Inventors: Kentaro SHIRAI, Yusuke TAKAHASHI, Masatoshi YANAGIDA, Shigeki IWANAGA, Tomokazu YOSHIDA, Yuichi IJIRI
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Patent number: 10156510Abstract: A particle imaging apparatus comprises a flow path comprising a first flow path section, a second flow path section connected downstream of the first flow path section, and a third flow path section that is branched from the first flow path section, a particle detection unit comprising a light source and a light detector, a particle sorting unit configured to adjust a flow direction of the particle, and a particle imaging unit configured to take an image of a particle that flows in the second flow path section. The flow path is structured such that a cross-sectional area of the second flow path section is greater than a cross-sectional area of the first flow path section. The first flow path section and the second flow path section are disposed so as to be linearly aligned.Type: GrantFiled: February 27, 2017Date of Patent: December 18, 2018Assignee: SYSMEX CORPORATIONInventors: Seiichiro Tabata, Masaki Ishisaka, Kenji Akama, Yoshinobu Miura, Masatoshi Yanagida
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Publication number: 20180292385Abstract: Disclosed is a method for measuring an immunostimulatory response of an immune cell, including (i) bringing a measurement target immune cell into contact with an immunostimulator, (ii) forming a contact surface with a substance different from the measurement target immune cell on the measurement target immune cell, (iii) bringing the measurement target immune cell into contact with a capturing body that binds to a surface antigen on the contact surface and is capable of generating an optical signal, (iv) detecting the optical signal generated from the capturing body, and (v) determining whether or not the measurement target immune cell from which the contact surface has been eliminated before detecting the optical signal has an immunostimulatory response, based on the detected optical signal.Type: ApplicationFiled: April 10, 2018Publication date: October 11, 2018Applicant: SYSMEX CORPORATIONInventors: Masatoshi Yanagida, Keiko Miwa, Yuma Oka
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Publication number: 20170227446Abstract: A particle imaging apparatus comprises a flow path comprising a first flow path section, a second flow path section connected downstream of the first flow path section, and a third flow path section that is branched from the first flow path section, a particle detection unit comprising a light source and a light detector, a particle sorting unit configured to adjust a flow direction of the particle, and a particle imaging unit configured to take an image of a particle that flows in the second flow path section. The flow path is structured such that a cross-sectional area of the second flow path section is greater than a cross-sectional area of the first flow path section. The first flow path section and the second flow path section are disposed so as to be linearly aligned.Type: ApplicationFiled: February 27, 2017Publication date: August 10, 2017Inventors: Seiichiro TABATA, Masaki ISHISAKA, Kenji AKAMA, Yoshinobu MIURA, Masatoshi YANAGIDA
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CELL SELECTION METHOD, CELL DETECTION METHOD, CELL SELECTION APPARATUS, AND CELL DETECTION APPARATUS
Publication number: 20170152552Abstract: Disclosed is a cell selection method including a sample preparation step of preparing a sample by performing staining of nucleic acid in each of cells by a first fluorescent dye; and hybridization with respect to an evaluation target region in DNA in each cell by an evaluation probe including a second fluorescent dye; a light receiving step of applying light to the sample and receiving fluorescence from the first fluorescent dye and fluorescence from the second fluorescent dye; and a selection step of selecting an analysis target cell on the basis of intensity of the fluorescence from the first fluorescent dye and intensity of the fluorescence from the second fluorescent dye, wherein the first fluorescent dye is a dye that emits fluorescence having a first wavelength, and the second fluorescent dye is a dye that emits fluorescence having a second wavelength different from the first wavelength.Type: ApplicationFiled: November 29, 2016Publication date: June 1, 2017Inventors: Yusuke TAKAHASHI, Masatoshi YANAGIDA -
Publication number: 20160340636Abstract: A cell detection device and a cell detection method is provided with which it is possible to efficiently acquire images of cells to be measured. Cell detection device comprises flow cell through which a measurement specimen that contains particles is caused to flow, particle detector for detecting the particles in the measurement specimen supplied to flow cell, particle sorter for sorting particles that satisfy a detection condition and other particles on the basis of the result of detection performed by particle detector, specimen supply part for supplying, to flow cell, an image-capture specimen that includes detection-condition-satisfying particles that have been sorted by particle sorter, and particle-image-capture part for capturing images of the particles in the sorted image-capture specimen supplied to flow cell.Type: ApplicationFiled: May 19, 2016Publication date: November 24, 2016Applicant: SYSMEX CORPORATIONInventors: Seiichiro TABATA, Masatoshi YANAGIDA
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Patent number: 9410208Abstract: The present invention relates to a marker allowing specific detection of human IL-17-producing helper T-cells (human Th17 cells), a method for specifically detecting human Th17 cells and a reagent for detecting human Th17 cells.Type: GrantFiled: April 23, 2013Date of Patent: August 9, 2016Assignee: SYSMEX CORPORATIONInventors: Satoshi Tanaka, Hitoshi Uga, Masafumi Ikeda, Yoshiaki Miyamoto, Masatoshi Yanagida, Masakazu Kadowaki, Takahiro Okazawa, Hirokazu Kurata
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Publication number: 20130309692Abstract: The present invention relates to polynucleotide markers and protein markers for detecting human follicular helper T cells. The present invention also relates to methods for detecting human follicular helper T cells using the markers.Type: ApplicationFiled: May 10, 2013Publication date: November 21, 2013Applicant: SYSMEX CORPORATIONInventors: Masatoshi YANAGIDA, Hitoshi UGA, Takahiro OKAZAWA, Yoshiaki MIYAMOTO, Masafumi IKEDA, Hirokazu KURATA
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Publication number: 20130267435Abstract: The present invention relates to a marker allowing specific detection of human IL-17-producing helper T-cells (human Th17 cells), a method for specifically detecting human Th17 cells and a reagent for detecting human Th17 cells.Type: ApplicationFiled: April 23, 2013Publication date: October 10, 2013Applicant: SYSMEX CORPORATIONInventors: Satoshi TANAKA, Hitoshi UGA, Masafumi IKEDA, Yoshiaki MIYAMOTO, Masatoshi YANAGIDA, Masakazu KADOWAKI, Takahiro OKAZAWA, Hirokazu KURATA