Abstract: Provided are a method for predicting a cell culture result, a culture result prediction program, and a culture result prediction apparatus that predict a culture result of an entire culture process. The method for predicting a cell culture result includes receiving a culture environment including a culture medium composition and a culture condition for culturing cells; predicting a biological behavior amount on the basis of the culture environment; changing a cell environment on the basis of the predicted biological behavior amount; repeating prediction of the biological behavior amount and change of the cell environment on the basis of the changed cell environment; and outputting the changed cell environment. Further, a culture result prediction program for executing the method for predicting a cell culture result, and a culture result prediction apparatus are provided.

Abstract: Provided are a method for creating a cell mathematical model capable of evaluating characteristics of cells from experimental data, a program, and a creation apparatus. Also provided are a method for determining the created cell mathematical model, a program, and a determination apparatus. The method for creating a mathematical model receives culture data of the cells, extracts feature values of cell activity from the culture data, creates a cell mathematical model from the feature values, and outputs the cell mathematical model. The method for determining the created cell mathematical model determines that estimated culture data calculated using the cell mathematical model reflects the culture data.

Abstract: Provided is an optimal solution determination method for determining optimality of a solution in a combinatorial optimization problem using a computer, including uniformly extracting a plurality of solutions in a solution space of the combinatorial optimization problem as a plurality of first solutions, and estimating a maximum evaluation value in a case where solutions of a number that exceeds the number of the plurality of first solutions are assumed, on the basis of a plurality of first evaluation values respectively corresponding to the plurality of first solutions that are uniformly extracted, as a first maximum evaluation value Z.

Abstract: Provided is a design method for dividing primer pairs into reaction containers, the design method showing an optimum division example. The design method for dividing primer pairs into reaction containers has a design step of, for a plurality of target nucleic acids, designing a plurality of primer pairs each composed of two types of primers, an evaluation step of evaluating non-specific amplification inducibility between the primer pairs, and an assignment step of performing an assignment to the reaction containers, based on the non-specific amplification inducibility, such that primer pairs having the non-specific amplification inducibility are not present in the same reaction container.

Abstract: The present invention is to provide a multi-class classification method, a multi-class classification program, and a multi-class classification device which can robustly and highly accurately classify a sample having a plurality of feature amounts into any of a plurality of classes based on a value of a part of the selected feature amount. In addition, the present invention is to provide a feature amount selection method, a feature amount selection program, a feature amount selection device, and a feature amount set used for such multi-class classification. The present invention handles a multi-class classification problem involving feature amount selection. The feature amount selection is a method of literally selecting in advance a feature amount needed for each subsequent processing (particularly, the multi-class classification in the present invention) from among a large number of feature amounts included in a sample.

Abstract: Provided is an information processing device that performs display control to display a vessel image obtained by imaging a vessel in which a cell is seeded on a display and includes at least one processor. The processor acquires two or more vessel images obtained by imaging the same vessel at different dates and times and displays the acquired two or more vessel images on the display such that different imaging dates and times are distinguishable.

Abstract: An information processing device detects a cell candidate region for determining a unity of a cell from a vessel image obtained by imaging a vessel in which the cell is seeded and includes at least one processor. The processor performs an acquisition process of acquiring the vessel image, performs a detection process of detecting a cell region including the cell and a cell-like region including an object similar to the cell as the cell candidate regions from the acquired vessel image, and an output process of outputting information indicating the detected cell candidate regions.

Abstract: There is provided an operation method for an information processing apparatus, the operation method being performed by a processor, the operation method including: an acquisition process for acquiring pieces of annotation information added to each of a plurality of biomarkers related to biological samples; a deriving process for deriving an evaluation value of each of the plurality of biomarkers on the basis of the pieces of annotation information; and a selection process for selecting on the basis of the evaluation value, measurement target biomarkers from among the plurality of biomarkers.

Abstract: Provided are a biomarker identifying method including (1) to (4) and an application thereof. (1) An evaluation value for each of a plurality of biomarkers is derived based on annotation information imparted to each of biomarkers, and a measurement target biomarker is selected based on the evaluation value. (2) The evaluation data of the measurement target biomarker is acquired from the cell A and/or the culture system, before the start of culture of the cell A and/or during the culture. (3) The evaluation data of the discrimination marker of the B cell is acquired from the cell A and/or the culture system, at the final stage of the culture of the cell A and/or after the end of the culture. (4) At least one biomarker indicating characteristics of the cell A is identified from among the measurement target biomarkers, based on the data obtained from (2) and (3).

Abstract: An object of the present invention is to provide a method of producing a pluripotent stem cell capable of differentiating into a specific cell. According to the present invention, there is provided a method for producing a pluripotent stem cell capable of differentiating into a specific cell, the method including (i) a step of measuring, in a pluripotent stem cell as a sample, a phenotype associated with induction of epithelial-mesenchymal transition before differentiation induction; and (ii) a step of acquiring a pluripotent stem cell capable of differentiating into a specific cell using the measured phenotype as an indicator. According to the present invention, there are further provided a method of selecting a pluripotent stem cell capable of differentiating into a specific cell; a pluripotent stem cell capable of differentiating into a specific cell; a production method of a differentiated cell; a differentiated cell; and a method for quality evaluation of a pluripotent stem cell.

Abstract: The present invention is to provide a multi-class classification method, a multi-class classification program, and a multi-class classification device which select a feature amount and classify a sample into any of a plurality of classes based on a value of the selected feature amount, and a feature amount selection method, a feature amount selection device, and a feature amount set which are used for such multi-class classification. The present invention handles a multi-class classification problem involving feature amount selection. The feature amount selection is a method of literally selecting in advance a feature amount needed for each subsequent processing (particularly, the multi-class classification in the present invention) from among a large number of feature amounts included in a sample. The multi-class classification is a discrimination problem that decides which of a plurality of classes a given unknown sample belongs to.

Abstract: Provided is a method for determining a genetic condition of a fetus, the method including: an objective region selection step of selecting an objective region for determining the genetic condition, from regions on a human genome; a step of isolating a single cell from a maternal blood sample; a step of extracting genomic DNA from the single cell; a step of performing PCR amplification on the objective region using a primer set designed so as to perform the PCR amplification on the objective region using genomic DNA extracted from the single cell as a template; and a DNA sequencing step of decoding a DNA base sequence of a PCR amplification product of the objective region, in which the primer set designed so as to perform the PCR amplification on the objective region is designed through a method for designing a primer set used for the polymerase chain reaction, the method for designing a primer set including a first stage selection step based on a local alignment score and a second stage selection step based o

Abstract: An optimal solution search method for searching for an optimal solution in a combinatorial optimization problem using a computer, includes: acquiring a first solution candidate; and enumerating and indexing solution candidate groups of which the degree of divergence from the first solution candidate is equal to or smaller than a predetermined range; equally extracting second solution candidates from the enumerated and indexed solution candidate groups assigning evaluation values to the second solution candidates; and determining whether the search for the optimal solution is terminated on the basis of the evaluation value. In a case where the search for the optimal solution is not terminated, the current first solution candidate is updated as the best second solution candidate selected from the second solution candidates, and the processes are repeated until the search for the first optimal solution is terminated.

Abstract: An object of the present invention is to provide a producing method for a pluripotent stem cell capable of differentiating into a specific cell. According to the present invention, there is provided a producing method for a pluripotent stem cell capable of differentiating into a specific cell. According to the present invention, there is provided a pluripotent stem cell capable of differentiating into a specific cell. According to the present invention, there is provided a producing method for a differentiated cell, and a differentiated cell. According to the present invention, there is provided a method for quality evaluation of a pluripotent stem cell. Further, according to the present invention, there is provided a method of screening a pluripotent stem cell capable of differentiating into a specific cell.

Abstract: Provided is an information processing apparatus including an acquiring unit that acquires cell information indicating a state of a cell from production of a pluripotent stem cell to differentiation of the pluripotent stem cell into a specific differentiated cell by differentiation induction, and process history information indicating a history of a processing process for obtaining the differentiated cell, and a deriving unit that derives differentiation potency information indicating differentiation potency of the pluripotent stem cell based on the cell information and the process history information which are acquired by the acquiring unit.

Abstract: Provided is an optimal solution determination method for determining optimality of a solution in a combinatorial optimization problem using a computer, including uniformly extracting a plurality of solutions in a solution space of the combinatorial optimization problem as a plurality of first solutions, and estimating a maximum evaluation value in a case where solutions of a number that exceeds the number of the plurality of first solutions are assumed, on the basis of a plurality of first evaluation values respectively corresponding to the plurality of first solutions that are uniformly extracted, as a first maximum evaluation value Z.

Abstract: An optimal solution search method for searching for an optimal solution in a combinatorial optimization problem using a computer, includes: acquiring a first solution candidate; and enumerating and indexing solution candidate groups of which the degree of divergence from the first solution candidate is equal to or smaller than a predetermined range; equally extracting second solution candidates from the enumerated and indexed solution candidate groups assigning evaluation values to the second solution candidates; and determining whether the search for the optimal solution is terminated on the basis of the evaluation value. In a case where the search for the optimal solution is not terminated, the current first solution candidate is updated as the best second solution candidate selected from the second solution candidates, and the processes are repeated until the search for the first optimal solution is terminated.

Abstract: Provided is a method for determining a genetic condition of a fetus, the method including: an objective region selection step of selecting an objective region for determining the genetic condition, from regions on a human genome; a step of isolating a single cell from a maternal blood sample; a step of extracting genomic DNA from the single cell; a step of performing PCR amplification on the objective region using a primer set designed so as to perform the PCR amplification on the objective region using genomic DNA extracted from the single cell as a template; and a DNA sequencing step of decoding a DNA base sequence of a PCR amplification product of the objective region, in which the primer set designed so as to perform the PCR amplification on the objective region is designed through a method for designing a primer set used for the polymerase chain reaction, the method for designing a primer set including a first stage selection step based on a local alignment score and a second stage selection step based o

Abstract: The security of source code is maintained when computer software is analyzed. To achieve this, computer software to undergo analysis is obfuscated in a client computer. The obfuscated computer software is transmitted to a server computer. Software analysis is performed in the server computer and data representing the result of this analysis is transmitted to the client computer. Since the data representing the result of analysis has thus been obfuscated, the contents of the computer software cannot be ascertained in the server computer. The obfuscated data representing the result of analysis is restored to the original in the client computer.