Abstract: Provided herein are plants and plant parts comprising reduced saponin content. The plants and plant parts can have a genetic mutation that decreases the beta-amyrin synthase (BAS) activity, one or more mutations in at least one BAS gene or homolog or in its regulatory region, decreased level or activity of the BAS gene or BAS protein, and/or improved flavor characteristics. Also provided herein are compositions and methods of producing such plants and plant parts, and plant products including plant protein compositions comprising reduced saponin content and/or improved flavor characteristics.

Abstract: Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

Abstract: Organic acid-producing microorganisms and methods of using same. The organic acid-producing microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid, acrylic acid, propionic acid, lactic acid, and others. Further modifications to the microorganisms increase production of such organic acids as 3-hydroxypropionic acid, lactate, and others. Methods of producing such organic acids as 3-hydroxypropionic acid, lactate, and others with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers are also provided.

Abstract: Organic acid-tolerant microorganisms and methods of using same. The organic acid-tolerant microorganisms comprise modifications that reduce or ablate AcsA activity or AcsA homolog activity. The modifications increase tolerance of the microorganisms to such organic acids as 3-hydroxypropionic acid (3HP), acrylic acid, and propionic acid. Further modifications to the microorganisms such as increasing expression of malonyl-CoA reductase and/or acetyl-CoA carboxylase provide or increase the ability of the microorganisms to produce 3HP. Methods of generating an organic acid with the modified microorganisms are provided. Methods of using acsA or homologs thereof as counter-selectable markers include replacing acsA or homologs thereof in cells with genes of interest and selecting for the cells comprising the genes of interest with amounts of organic acids effective to inhibit growth of cells harboring acsA or the homologs.

Abstract: The invention provides an isolated haloalkaliphilic microorganism designated as strain sapolanicus belonging to the genus Halanaerobium, which is capable of producing hydrogen from biomass. Methods of producing biohydrogen comprising the fermentation of the microorganism with alkaline pretreated biomass are also provided. Fermentation is preferably carried out without neutralization of the pretreated biomass and at a pH of greater than or equal to about 10.

Abstract: The invention provides an isolated haloalkaliphilic microorganism designated as strain sapolanicus belonging to the genus Halanaerobium, which is capable of producing hydrogen from biomass. Methods of producing biohydrogen comprising the fermentation of the microorganism with alkaline pretreated biomass are also provided. Fermentation is preferably carried out without neutralization of the pretreated biomass and at a pH of greater than or equal to about 10.

Abstract: The invention provides an isolated haloalkaliphilic microorganism designated as strain sapolanicus belonging to the genus Halanaerobium, which is capable of producing hydrogen from biomass. Methods of producing biohydrogen comprising the fermentation of the microorganism with alkaline pretreated biomass are also provided. Fermentation is preferably carried out without neutralization of the pretreated biomass and at a pH of greater than or equal to about 10.

Abstract: The invention provides an isolated haloalkaliphilic microorganism designated as strain sapolanicus belonging to the genus Halanaerobium, which is capable of producing hydrogen from biomass. Methods of producing biohydrogen comprising the fermentation of the microorganism with alkaline pretreated biomass are also provided. Fermentation is preferably carried out without neutralization of the pretreated biomass and at a pH of greater than or equal to about 10.