Abstract: A system and methods of characterizing a population of a virus, bacteriophage, or other microbes in a microbiome that includes the steps of separating a sample of microbiota into more than one fraction by continuous capillary zone electrophoresis based on the physiochemical properties of the microbes in the microbiota using a constant voltage applied to the sample during the continuous zone electrophoresis. At least one separated fraction includes an intact virus, bacteriophage, or other microbe that may be visualized and/or directly sequenced to characterize the population of the virus, bacteriophage, or other microbe in the microbiome.

Abstract: A system and methods of characterizing a population of a virus, bacteriophage, or other microbes in a microbiome that includes the steps of separating a sample of microbiota into more than one fraction by continuous capillary zone electrophoresis based on the physiochemical properties of the microbes in the microbiota using a constant voltage applied to the sample during the continuous zone electrophoresis. At least one separated fraction includes an intact virus, bacteriophage, or other microbe that may be visualized and/or directly sequenced to characterize the population of the virus, bacteriophage, or other microbe in the microbiome.

Abstract: Capillary electrophoresis fractionation of an environmental microbiota segregates high abundance microbes from lower abundance species, and results in a three-fold increase in the number of 16s rRNA OTUs that map to known species. However, most of the bacteria are found in a few wells, although reasonably large numbers of OTUs were found in over half the wells.

Abstract: Provided is a game with moveable play space that allows a player to modify the play space. Spaces within the play space may be wildcard spaces that initiate modification. Game play space modifications include manipulations such as column shuffling, rotating, and flip-flopping. The selection of which portion of the game play space to modify may be made by the players of the game, by the space on which a game piece resides, or by the particular type of game piece that is moved or used. In some embodiments, different types of players govern modification to the game play space. In volumetric games, certain volumetric portions are exchanged with one another during the modification. Modification of the game play space is performed along various surfaces within the volumetric game play space, or within certain individual game sub-volumes that together integrate to form the entire volumetric game play space.

Abstract: A game with moveable play space. The invention allows any user playing the game to modify the play space at any number of given times and in response to any number of different conditions. Certain spaces (tiles or blocks) within the play space are wildcard spaces that independently initiates modification of the game play space. Any game play space can benefit from the invention, including conventional game boards including chess and checker boards. The modifications of the game play space include any number of manipulations including column shuffling, rotating, flip-flopping, and other types of manipulation. The selection of which portion of the game play space is made by the players of the game. If desired, the selection of which portion of the game play space is governed by the space on which a game piece resides, or the particular type of game piece that is being moved or used in that game.