Abstract: In one example, this disclosure describes a method including receiving, at a central controller of an air mattress system, a plurality of air pressure value, determining a plurality of average values using the plurality of the received air pressure values, calculating a difference value between a first one of the plurality of average values and a second one of the plurality of average values, comparing the difference value to a threshold value, determining, based on the comparison, whether a user of the air mattress system moved.

Abstract: A method (400) for coating a tip (106) of an aerofoil (100) is provided. The method (400) includes depositing a layer of nickel-based gamma/gamma prime chemistry (112) on the tip (106) of the aerofoil (100). The method (400) further includes depositing plurality of abrasive particles (114) on the layer of nickel-based gamma/gamma prime chemistry (112) to form a coating matrix (116). The method (400) further includes heating the tip (106) of the aerofoil (100) at a predetermined temperature in order to perform heat treatment of the coating matrix (116) and increase the strength of the coating (110) on the tip (106) of the aerofoil (100).

Abstract: The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.

Abstract: Devices and methods for sterile sampling from automated cell engineering systems are provided. Sterile sampling devices are configured to maintain sterility of a sample reservoir during intake and expulsion of fluids or other material to the sterile sampling devices. Methods provided herein employ sterile sampling devices to achieve the sterile withdrawal and sterile injection of materials and fluids to and from an automated cell engineering system.

Abstract: The present disclosure provides devices and associated methods for temperature monitoring and control in automated biological material engineering systems, including cell engineering systems. The devices and methods utilize measurement of internal temperatures in an automated system to map temperatures during the various processes carried out in the systems.

Abstract: The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.

Abstract: The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines, or packaging cells, within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.

Abstract: The present disclosure provides methods for assessing and optimizing cellular quality of a cell-based therapy that is being produced in an automated cell engineering system. The methods suitably include monitoring molecular characteristics of the cells before, during, and after the automated process to provide feedback to the process parameters. In embodiments, the cells being produced are Chimeric Antigen Receptor (CAR) T-cells.

Abstract: The present disclosure provides cell culture chambers for use in automated cell engineering systems, and in particular, cell culture chambers that include improved cell-contacting surfaces. Improved cell-contacting surfaces can include a surface coating that promotes cell growth, adherence, differentiation, maintenance of phenotype, and/or improves transduction; a cell-contacting surface comprising a non-porous, gas-permeable material; as well as other modifications to the cell-contacting surfaces. Cassettes comprising the cell culture chambers are also provided.

Abstract: The present disclosure provides an automated method of producing viral vectors, utilizing engineered viral vector-producing cell lines within a fully-enclosed cell engineering system. Exemplary viral vectors that can be produced include lentivirus vectors, adeno-associated virus vectors, baculovirus vectors and retrovirus vectors.