Abstract: An isolated polynucleotide encoding a modified luciferase polypeptide and substrates. The OgLuc variant polypeptide has at least 60% amino acid sequence identity to SEQ ID NO: 1 and at least one amino acid substitution at a position corresponding to an amino acid in SEQ ID NO: 1. The OgLuc variant polypeptide has at least one of enhanced luminescence, enhanced signal stability, and enhanced protein stability relative to the corresponding polypeptide of the wild-type Oplophorus luciferase.

Abstract: An ozonization system (100, 200 or 300) for washing and cleaning objects (71) is described. The system includes a container (110, 210 or 310), an ozonization unit (50) and a diffuser assembly. The container can be a standard kitchen sink, a commercial sink, enclosure (30) or an airtight box (410). The ozonization unit contains a pump (8), an ozone generator (6) and a water separator (162, 262 or 362) which are all controlled by a control circuit (5). The diffuser assembly in one embodiment is a diffuser wand assembly which fits within the standard spray opening (110A) of a kitchen sink. The diffuser tip (129) is inserted into the container and the ozone is diffused into the container through the diffuser tip. In another embodiment, the container has a diffuser plate (214 or 314) spaced apart from the bottom. The ozone is inserted into the gap (213 or 313) between the diffuser plate and the bottom of the container. The ozone is diffused from the gap through the diffuser plate into the container.