Abstract: The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.

Abstract: The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.

Abstract: This invention provides a fluidic device comprising a diaphragm valve having a fluidics layer, an actuation layer and an elastic layer between the fluidics layer and the actuation layer, the elastic layer having a diaphragm that is mechanically sealed against the fluidics layer and the actuation layer by a sealing ring in the actuation layer.

Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

Abstract: The invention provides for devices and methods for interfacing microchips to cartridges and pneumatic manifolds. The cartridges, microchips, and pneumatic manifolds can be integrated with downstream preparation devices, such as thermal regulating devices and separation and analysis devices.

Abstract: The invention provides for devices and methods for interfacing microchips to cartridges and pneumatic manifolds. The cartridges, microchips, and pneumatic manifolds can be integrated with downstream preparation devices, such as thermal regulating devices and separation and analysis devices.

Abstract: The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.

Abstract: The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.

Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post-reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft3. and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

Abstract: The invention provides for devices and methods for interfacing microchips to cartridges and pneumatic manifolds. The cartridges, microchips, and pneumatic manifolds can be integrated with downstream preparation devices, such as thermal regulating devices and separation and analysis devices.

Abstract: A connector is provided for selectively coupling one or more lines or wires (19?, 19?) to a receiving and/or transporting device (27). The connector comprises at least one line or wire receiving ferrule (7) having a plurality of line and/or wire receiving holes (17?-17?) each capable of receiving one of said one or more lines or wires (19?, 19?), wherein said connector further comprises a body (3) for receiving said ferrule (7) and clamping means (9, 11) for exerting a force on said ferrule (7) in order to simultaneously exert a line or wire clamping force on all said line and/or wire receiving holes (17?-17?). In this way all the fluid lines or wires can be clamped at the same time.

Abstract: A connector is provided for selectively coupling one or more lines or wires (19?, 19?) to a receiving and/or transporting device (27). The connector comprises at least one line or wire receiving ferrule (7) having a plurality of line and/or wire receiving holes (17?-17?) each capable of receiving one of said one or more lines or wires (19?, 19?), wherein said connector further comprises a body (3) for receiving said ferrule (7) and clamping means (9, 11) for exerting a force on said ferrule (7) in order to simultaneously exert a line or wire clamping force on all said line and/or wire receiving holes (17?-17?). In this way all the fluid lines or wires can be clamped at the same time.

Abstract: A connector is provided for selectively coupling one or more lines or wires (19?, 19?) to a receiving and/or transporting device (27). The connector comprises at least one line or wire receiving ferrule (7) having a plurality of line and/or wire receiving holes (17?-17?) each capable of receiving one of said one or more lines or wires (19?, 19?), wherein said connector further comprises a body (3) for receiving said ferrule (7) and clamping means (9, 11) for exerting a force on said ferrule (7) in order to simultaneously exert a line or wire clamping force on all said line and/or wire receiving holes (17?-17?). In this way all the fluid lines or wires can be clamped at the same time.

Abstract: The present invention relates to a method for reducing the solvent content of a sample in a microchannel or other microfluidic structure comprising the steps of 1) freezing said sample and 2) exposing the frozen sample to a low solvent vapour pressure atmosphere.

Abstract: The present invention relates to mass spectrometer target slides (1) which are provided with pits (9a-9n) on the sample receiving surface (5). The sample receiving surface (5) and the rims (11a-11n) of the pit (9a-9n) are coated with a hydrophobic layer 17. The at least one pit (9a-9n) has a width or diameter Ø1 which is less than the width of a drop applied to the pit (9a-9n).

Abstract: A connector is provided for selectively coupling one or more lines or wires (19?, 19?) to a receiving and/or transporting device (27). The connector comprises at least one line or wire receiving ferrule (7) having a plurality of line and/or wire receiving holes (17?-17?) each capable of receiving one of said one or more lines or wires (19?, 19?), wherein said connector further comprises a body (3) for receiving said ferrule (7) and clamping means (9, 11) for exerting a force on said ferrule (7) in order to simultaneously exert a line or wire clamping force on all said line and/or wire receiving holes (17?-17?). In this way all the fluid lines or wires can be clamped at the same time.